EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.61 | expressed in Escherichia coli JM109 cells | Yarrowia lipolytica |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.1.61 | D423A | the mutant shows reduced activity compared to the wild type enzyme | Yarrowia lipolytica |
2.3.1.61 | D423DEL | the mutant shows reduced activity compared to the wild type enzyme | Yarrowia lipolytica |
2.3.1.61 | D423E | the mutant shows reduced activity compared to the wild type enzyme | Yarrowia lipolytica |
2.3.1.61 | D423G | the mutant shows reduced activity compared to the wild type enzyme | Yarrowia lipolytica |
2.3.1.61 | D423S | the mutant shows reduced activity compared to the wild type enzyme | Yarrowia lipolytica |
2.3.1.61 | H419A | the mutant shows reduced activity compared to the wild type enzyme | Yarrowia lipolytica |
2.3.1.61 | H419D | the mutant shows reduced activity compared to the wild type enzyme | Yarrowia lipolytica |
2.3.1.61 | H419del | inactive | Yarrowia lipolytica |
2.3.1.61 | H419G | the mutant shows reduced activity compared to the wild type enzyme | Yarrowia lipolytica |
2.3.1.61 | H419S | the mutant shows reduced activity compared to the wild type enzyme | Yarrowia lipolytica |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.61 | 0.024 | - |
DL-dihydrolipoamide | mutant enzyme H419S, at pH 8.0 and 30°C | Yarrowia lipolytica | |
2.3.1.61 | 0.025 | - |
DL-dihydrolipoamide | mutant enzyme H419DEL, at pH 8.0 and 30°C | Yarrowia lipolytica | |
2.3.1.61 | 0.026 | - |
DL-dihydrolipoamide | mutant enzyme H419A, at pH 8.0 and 30°C | Yarrowia lipolytica | |
2.3.1.61 | 0.026 | - |
DL-dihydrolipoamide | mutant enzyme H419G, at pH 8.0 and 30°C | Yarrowia lipolytica | |
2.3.1.61 | 0.0274 | - |
DL-dihydrolipoamide | wild type enzyme, at pH 8.0 and 30°C | Yarrowia lipolytica | |
2.3.1.61 | 0.03 | - |
DL-dihydrolipoamide | mutant enzyme H419D, at pH 8.0 and 30°C | Yarrowia lipolytica | |
2.3.1.61 | 0.043 | - |
DL-dihydrolipoamide | mutant enzyme D423S, at pH 8.0 and 30°C | Yarrowia lipolytica | |
2.3.1.61 | 0.052 | - |
DL-dihydrolipoamide | mutant enzyme D423G, at pH 8.0 and 30°C | Yarrowia lipolytica | |
2.3.1.61 | 0.062 | - |
DL-dihydrolipoamide | mutant enzyme D423A, at pH 8.0 and 30°C | Yarrowia lipolytica | |
2.3.1.61 | 0.062 | - |
DL-dihydrolipoamide | mutant enzyme D423DEL, at pH 8.0 and 30°C | Yarrowia lipolytica | |
2.3.1.61 | 0.09 | - |
DL-dihydrolipoamide | mutant enzyme D423E, at pH 8.0 and 30°C | Yarrowia lipolytica |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.61 | Yarrowia lipolytica | - |
- |
- |
2.3.1.61 | Yarrowia lipolytica WSH-Z06 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.61 | Ni-NTA agarose column chromatography | Yarrowia lipolytica |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.61 | succinyl-CoA + DL-dihydrolipoamide | - |
Yarrowia lipolytica | CoA + S-succinyldihydrolipoamide | - |
? | |
2.3.1.61 | succinyl-CoA + DL-dihydrolipoamide | - |
Yarrowia lipolytica WSH-Z06 | CoA + S-succinyldihydrolipoamide | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.61 | dihydrolipoamide succinyltransferase | - |
Yarrowia lipolytica |
2.3.1.61 | DLST | - |
Yarrowia lipolytica |
2.3.1.61 | KGD2 | - |
Yarrowia lipolytica |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.61 | 3 | - |
DL-dihydrolipoamide | mutant enzyme D423G, at pH 8.0 and 30°C | Yarrowia lipolytica | |
2.3.1.61 | 3 | - |
DL-dihydrolipoamide | mutant enzyme H419G, at pH 8.0 and 30°C | Yarrowia lipolytica | |
2.3.1.61 | 6 | - |
DL-dihydrolipoamide | mutant enzyme H419D, at pH 8.0 and 30°C | Yarrowia lipolytica | |
2.3.1.61 | 7 | - |
DL-dihydrolipoamide | mutant enzyme D423A, at pH 8.0 and 30°C | Yarrowia lipolytica | |
2.3.1.61 | 7 | - |
DL-dihydrolipoamide | mutant enzyme D423DEL, at pH 8.0 and 30°C | Yarrowia lipolytica | |
2.3.1.61 | 7 | - |
DL-dihydrolipoamide | mutant enzyme H419A, at pH 8.0 and 30°C | Yarrowia lipolytica | |
2.3.1.61 | 11 | - |
DL-dihydrolipoamide | mutant enzyme H419S, at pH 8.0 and 30°C | Yarrowia lipolytica | |
2.3.1.61 | 18 | - |
DL-dihydrolipoamide | mutant enzyme D423S, at pH 8.0 and 30°C | Yarrowia lipolytica | |
2.3.1.61 | 165 | - |
DL-dihydrolipoamide | mutant enzyme D423E, at pH 8.0 and 30°C | Yarrowia lipolytica | |
2.3.1.61 | 186 | - |
DL-dihydrolipoamide | wild type enzyme, at pH 8.0 and 30°C | Yarrowia lipolytica |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.61 | 7000 | - |
DL-dihydrolipoamide | wild type enzyme, at pH 8.0 and 30°C | Yarrowia lipolytica |