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Literature summary extracted from

  • Guo, H.; Madzak, C.; Du, G.; Zhou, J.
    Mutagenesis of conserved active site residues of dihydrolipoamide succinyltransferase enhances the accumulation of alpha-ketoglutarate in Yarrowia lipolytica (2016), Appl. Microbiol. Biotechnol., 100, 649-659 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.61 expressed in Escherichia coli JM109 cells Yarrowia lipolytica

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.61 D423A the mutant shows reduced activity compared to the wild type enzyme Yarrowia lipolytica
2.3.1.61 D423DEL the mutant shows reduced activity compared to the wild type enzyme Yarrowia lipolytica
2.3.1.61 D423E the mutant shows reduced activity compared to the wild type enzyme Yarrowia lipolytica
2.3.1.61 D423G the mutant shows reduced activity compared to the wild type enzyme Yarrowia lipolytica
2.3.1.61 D423S the mutant shows reduced activity compared to the wild type enzyme Yarrowia lipolytica
2.3.1.61 H419A the mutant shows reduced activity compared to the wild type enzyme Yarrowia lipolytica
2.3.1.61 H419D the mutant shows reduced activity compared to the wild type enzyme Yarrowia lipolytica
2.3.1.61 H419del inactive Yarrowia lipolytica
2.3.1.61 H419G the mutant shows reduced activity compared to the wild type enzyme Yarrowia lipolytica
2.3.1.61 H419S the mutant shows reduced activity compared to the wild type enzyme Yarrowia lipolytica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.61 0.024
-
DL-dihydrolipoamide mutant enzyme H419S, at pH 8.0 and 30°C Yarrowia lipolytica
2.3.1.61 0.025
-
DL-dihydrolipoamide mutant enzyme H419DEL, at pH 8.0 and 30°C Yarrowia lipolytica
2.3.1.61 0.026
-
DL-dihydrolipoamide mutant enzyme H419A, at pH 8.0 and 30°C Yarrowia lipolytica
2.3.1.61 0.026
-
DL-dihydrolipoamide mutant enzyme H419G, at pH 8.0 and 30°C Yarrowia lipolytica
2.3.1.61 0.0274
-
DL-dihydrolipoamide wild type enzyme, at pH 8.0 and 30°C Yarrowia lipolytica
2.3.1.61 0.03
-
DL-dihydrolipoamide mutant enzyme H419D, at pH 8.0 and 30°C Yarrowia lipolytica
2.3.1.61 0.043
-
DL-dihydrolipoamide mutant enzyme D423S, at pH 8.0 and 30°C Yarrowia lipolytica
2.3.1.61 0.052
-
DL-dihydrolipoamide mutant enzyme D423G, at pH 8.0 and 30°C Yarrowia lipolytica
2.3.1.61 0.062
-
DL-dihydrolipoamide mutant enzyme D423A, at pH 8.0 and 30°C Yarrowia lipolytica
2.3.1.61 0.062
-
DL-dihydrolipoamide mutant enzyme D423DEL, at pH 8.0 and 30°C Yarrowia lipolytica
2.3.1.61 0.09
-
DL-dihydrolipoamide mutant enzyme D423E, at pH 8.0 and 30°C Yarrowia lipolytica

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.61 Yarrowia lipolytica
-
-
-
2.3.1.61 Yarrowia lipolytica WSH-Z06
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.61 Ni-NTA agarose column chromatography Yarrowia lipolytica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.61 succinyl-CoA + DL-dihydrolipoamide
-
Yarrowia lipolytica CoA + S-succinyldihydrolipoamide
-
?
2.3.1.61 succinyl-CoA + DL-dihydrolipoamide
-
Yarrowia lipolytica WSH-Z06 CoA + S-succinyldihydrolipoamide
-
?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.61 dihydrolipoamide succinyltransferase
-
Yarrowia lipolytica
2.3.1.61 DLST
-
Yarrowia lipolytica
2.3.1.61 KGD2
-
Yarrowia lipolytica

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.61 3
-
DL-dihydrolipoamide mutant enzyme D423G, at pH 8.0 and 30°C Yarrowia lipolytica
2.3.1.61 3
-
DL-dihydrolipoamide mutant enzyme H419G, at pH 8.0 and 30°C Yarrowia lipolytica
2.3.1.61 6
-
DL-dihydrolipoamide mutant enzyme H419D, at pH 8.0 and 30°C Yarrowia lipolytica
2.3.1.61 7
-
DL-dihydrolipoamide mutant enzyme D423A, at pH 8.0 and 30°C Yarrowia lipolytica
2.3.1.61 7
-
DL-dihydrolipoamide mutant enzyme D423DEL, at pH 8.0 and 30°C Yarrowia lipolytica
2.3.1.61 7
-
DL-dihydrolipoamide mutant enzyme H419A, at pH 8.0 and 30°C Yarrowia lipolytica
2.3.1.61 11
-
DL-dihydrolipoamide mutant enzyme H419S, at pH 8.0 and 30°C Yarrowia lipolytica
2.3.1.61 18
-
DL-dihydrolipoamide mutant enzyme D423S, at pH 8.0 and 30°C Yarrowia lipolytica
2.3.1.61 165
-
DL-dihydrolipoamide mutant enzyme D423E, at pH 8.0 and 30°C Yarrowia lipolytica
2.3.1.61 186
-
DL-dihydrolipoamide wild type enzyme, at pH 8.0 and 30°C Yarrowia lipolytica

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.1.61 7000
-
DL-dihydrolipoamide wild type enzyme, at pH 8.0 and 30°C Yarrowia lipolytica