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Literature summary extracted from

  • Sindhu, R.; Manonmani, H.K.
    Expression and characterization of recombinant L-asparaginase from Pseudomonas fluorescens (2018), Protein Expr. Purif., 143, 83-91 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.1 expression in Escherichia coli Pseudomonas fluorescens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.1 2-mercaptoethanol 1 mM, 10% inhibition Pseudomonas fluorescens
3.5.1.1 Co2+ 1 mM, 53 inhibition Pseudomonas fluorescens
3.5.1.1 Cu2+ 1 mM, 47inhibition Pseudomonas fluorescens
3.5.1.1 cysteine 1 mM, 13% inhibition Pseudomonas fluorescens
3.5.1.1 EDTA 1 mM, 19% inhibition Pseudomonas fluorescens
3.5.1.1 iodoacetamide 1 mM, 20% inhibition Pseudomonas fluorescens
3.5.1.1 N-bromosuccinimide 1 mM, 5% inhibition Pseudomonas fluorescens
3.5.1.1 Pb2+ 1 mM, 59inhibition Pseudomonas fluorescens
3.5.1.1 PMSF 1 mM, 63% inhibition Pseudomonas fluorescens
3.5.1.1 SDS 2%, % inhibition Pseudomonas fluorescens
3.5.1.1 Sodium azide 1 mM, 15% inhibition Pseudomonas fluorescens
3.5.1.1 Urea 1 mM, 13% inhibition Pseudomonas fluorescens
3.5.1.1 Zn2+ 1 mM, 54inhibition Pseudomonas fluorescens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.1 Mg2+ 1 mM, 149% of initial activity Pseudomonas fluorescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.1 140000
-
 gel filtration Pseudomonas fluorescens

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.1 Pseudomonas fluorescens
-
-
-
3.5.1.1 Pseudomonas fluorescens MTCC 8127
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.1 recombinant protein Pseudomonas fluorescens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.1 26
-
 pH 8.0, 37°C Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.1 L-asparagine + H2O
-
 Pseudomonas fluorescens L-aspartate + NH3
-
 ?
3.5.1.1 L-asparagine + H2O
-
 Pseudomonas fluorescens MTCC 8127 L-aspartate + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.1 tetramer 4 * 35000, SDS-PAGE Pseudomonas fluorescens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.1 37
-
-
 Pseudomonas fluorescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.1 7.5
-
-
 Pseudomonas fluorescens