Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Gontijo, F.A.; de Melo, A.T.; Pascon, R.C.; Fernandes, L.; Paes, H.C.; Alspaugh, J.A.; Vallim, M.A.
    The role of aspartyl aminopeptidase (Ape4) in Cryptococcus neoformans virulence and authophagy (2017), PLoS ONE, 12, e0177461 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.21 gene ape4, located on chromosome 5, sequence comparisons, recombinant expression of GFP-tagged enzyme in Cryptococcus neoformans Cryptococcus neoformans var. grubii

Protein Variants

EC Number Protein Variants Comment Organism
3.4.11.21 additional information random insertional mutagenesis, the ape4 mutants are sensitive to high temperature growth. ape4 Mutation affects multiple virulence factors in Cryptococcus neoformans Cryptococcus neoformans var. grubii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.11.21 cytosol
-
 Cryptococcus neoformans var. grubii 5829
-
3.4.11.21 vesicle Ape4 is localized within the vesicles formed in response to nitrogen starvation and high temperatures. The GFP-Ape4 fusion protein co-localized with intracytoplasmic vesicles during nitrogen depletion Cryptococcus neoformans var. grubii 31982
-

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.21 Cryptococcus neoformans var. grubii J9VNI6 i.e. Filobasidiella neoformans var. grubii, serotype A
-
3.4.11.21 Cryptococcus neoformans var. grubii ATCC 208821 J9VNI6 i.e. Filobasidiella neoformans var. grubii, serotype A
-
3.4.11.21 Cryptococcus neoformans var. grubii CBS 10515 J9VNI6 i.e. Filobasidiella neoformans var. grubii, serotype A
-
3.4.11.21 Cryptococcus neoformans var. grubii FGSC 9487 J9VNI6 i.e. Filobasidiella neoformans var. grubii, serotype A
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.21 additional information the APE4 expression pattern is influenced by nitrogen starvation and high temperature, APE4 expression is induced by the combination of nutrient and thermal stress Cryptococcus neoformans var. grubii
-

Synonyms

EC Number Synonyms Comment Organism
3.4.11.21 Ape4
-
 Cryptococcus neoformans var. grubii
3.4.11.21 Ape4 aspartyl aminopeptidase
-
 Cryptococcus neoformans var. grubii
3.4.11.21 Ape4 metalloprotease
-
 Cryptococcus neoformans var. grubii
3.4.11.21 aspartyl aminopeptidase
-
 Cryptococcus neoformans var. grubii
3.4.11.21 CNAG_01169
-
 Cryptococcus neoformans var. grubii

Expression

EC Number Organism Comment Expression
3.4.11.21 Cryptococcus neoformans var. grubii the APE4 expression pattern is influenced by nitrogen starvation and high temperature additional information
3.4.11.21 Cryptococcus neoformans var. grubii APE4 expression is induced by the combination of nutrient and thermal stress up

General Information

EC Number General Information Comment Organism
3.4.11.21 malfunction a Cryptococcus neoformans ape4 knockout mutant does not grow at 37°C, and also has defects in the expression of important virulence factors such as phospholipase production and capsule formation. The ape4 mutants are sensitive to high temperature growth. ape4 Mutation affects multiple virulence factors in Cryptococcus neoformans Cryptococcus neoformans var. grubii
3.4.11.21 physiological function Cryptococcus neoformans Ape4 activity is required by facultative intracellular pathogen to survive within macrophages, as well as for virulence in an animal model of cryptococcal infection. The enzyme is involved in autophagy. Cryptococcus neoformans autophagy-related genes are modulated during nitrogen starvation and thermal stress, overview The APE4 gene is involved in multi-stress resistance, aspartyl aminopeptidase encoded by APE4 is important during response to osmotic/salt stress Cryptococcus neoformans var. grubii