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Literature summary extracted from

  • Ellens, K.; Richardson, L.; Frelin, O.; Collins, J.; Ribeiro, C.; Hsieh, Y.; Mullen, R.; Hanson, A.
    Evidence that glutamine transaminase and omega-amidase potentially act in tandem to close the methionine salvage cycle in bacteria and plants (2015), Phytochemistry, 113, 160-169 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.117 expression in Escherichia coli Solanum lycopersicum
2.6.1.117 expression in Escherichia coli Bacillus subtilis
2.6.1.117 expression in Escherichia coli Zea mays
3.5.1.3
-
 Solanum lycopersicum
3.5.1.3
-
 Zea mays

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.117 4-(methylsulfanyl)-2-oxobutanoate
-
 Bacillus subtilis
2.6.1.117 4-(methylsulfanyl)-2-oxobutanoate
-
 Solanum lycopersicum
2.6.1.117 4-(methylsulfanyl)-2-oxobutanoate
-
 Zea mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.117 0.07
-
 4-(methylsulfanyl)-2-oxobutanoate pH 7.5, 25°C Zea mays
2.6.1.117 0.22
-
 4-(methylsulfanyl)-2-oxobutanoate pH 7.5, 25°C Solanum lycopersicum
2.6.1.117 1.91
-
 4-(methylsulfanyl)-2-oxobutanoate pH 7.5, 37°C Bacillus subtilis
3.5.1.3 5.31
-
 2-Oxoglutaramate pH 8.5, 37°C Zea mays
3.5.1.3 6.42
-
 2-Oxoglutaramate pH 8.5, 37°C Zea mays
3.5.1.3 6.49
-
 2-Oxoglutaramate pH 8.5, 37°C Solanum lycopersicum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.6.1.117 mitochondrion
-
 Zea mays 5739
-
2.6.1.117 plastid
-
 Zea mays 9536
-
3.5.1.3 chloroplast
-
 Zea mays 9507
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.3 2-oxoglutaramate + H2O Solanum lycopersicum glutamine transaminase and omega-amidase potentially act in tandem to close the methionine salvage cycle in bacteria and plants 2-oxoglutarate + NH3
-
 ?
3.5.1.3 2-oxoglutaramate + H2O Zea mays glutamine transaminase and omega-amidase potentially act in tandem to close the methionine salvage cycle in bacteria and plants 2-oxoglutarate + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.117 Bacillus subtilis O31665
-
-
2.6.1.117 Bacillus subtilis 168 O31665
-
-
2.6.1.117 Solanum lycopersicum
-
-
-
2.6.1.117 Zea mays A0A1D6I5Q5 cf. EC 2.6.1.1
-
3.5.1.3 Solanum lycopersicum
-
-
-
3.5.1.3 Zea mays B8A0E5
-
-
3.5.1.3 Zea mays B8A2V8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.3
-
 Solanum lycopersicum
3.5.1.3
-
 Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.117 L-glutamine + 4-(methylsulfanyl)-2-oxobutanoate
-
 Solanum lycopersicum 2-oxoglutaramate + L-methionine
-
 ?
2.6.1.117 L-glutamine + 4-(methylsulfanyl)-2-oxobutanoate
-
 Bacillus subtilis 2-oxoglutaramate + L-methionine
-
 ?
2.6.1.117 L-glutamine + 4-(methylsulfanyl)-2-oxobutanoate
-
 Zea mays 2-oxoglutaramate + L-methionine
-
 ?
2.6.1.117 L-glutamine + 4-(methylsulfanyl)-2-oxobutanoate
-
 Bacillus subtilis 168 2-oxoglutaramate + L-methionine
-
 ?
2.6.1.117 L-histidine + 4-(methylsulfanyl)-2-oxobutanoate
-
 Solanum lycopersicum 3-((1H-imidazo)-4-yl)-2-oxopropanoate + L-methionine
-
 ?
2.6.1.117 additional information enzyme is almost completely specific for glutamine as amino donor, other amino acids giving no more than 1% of the activity measured with glutamine Solanum lycopersicum ?
-
 ?
3.5.1.3 2-oxoglutaramate + H2O
-
 Solanum lycopersicum 2-oxoglutarate + NH3
-
 ?
3.5.1.3 2-oxoglutaramate + H2O
-
 Zea mays 2-oxoglutarate + NH3
-
 ?
3.5.1.3 2-oxoglutaramate + H2O glutamine transaminase and omega-amidase potentially act in tandem to close the methionine salvage cycle in bacteria and plants Solanum lycopersicum 2-oxoglutarate + NH3
-
 ?
3.5.1.3 2-oxoglutaramate + H2O glutamine transaminase and omega-amidase potentially act in tandem to close the methionine salvage cycle in bacteria and plants Zea mays 2-oxoglutarate + NH3
-
 ?
3.5.1.3 2-oxosuccinamate + H2O
-
 Solanum lycopersicum oxaloacetate + NH3
-
 ?
3.5.1.3 2-oxosuccinamate + H2O
-
 Zea mays oxaloacetate + NH3
-
 ?
3.5.1.3 succinamate + H2O
-
 Solanum lycopersicum succinate + NH3
-
 ?
3.5.1.3 succinamate + H2O
-
 Zea mays succinate + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.6.1.117 101251438
-
 Solanum lycopersicum
2.6.1.117 Solyc11g013170 locus name Solanum lycopersicum
2.6.1.117 ZEAMMB73_Zm00001d020696
-
 Zea mays
3.5.1.3 omega-amidase
-
 Solanum lycopersicum
3.5.1.3 omega-amidase
-
 Zea mays

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.117 0.12
-
 4-(methylsulfanyl)-2-oxobutanoate pH 7.5, 25°C Zea mays
2.6.1.117 0.15
-
 4-(methylsulfanyl)-2-oxobutanoate pH 7.5, 25°C Solanum lycopersicum
2.6.1.117 2.32
-
 4-(methylsulfanyl)-2-oxobutanoate pH 7.5, 37°C Bacillus subtilis
3.5.1.3 50.8
-
 2-Oxoglutaramate pH 8.5, 37°C Zea mays
3.5.1.3 63.1
-
 2-Oxoglutaramate pH 8.5, 37°C Zea mays
3.5.1.3 72.7
-
 2-Oxoglutaramate pH 8.5, 37°C Solanum lycopersicum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.6.1.117 0.38
-
 4-(methylsulfanyl)-2-oxobutanoate pH 7.5, 25°C Solanum lycopersicum
2.6.1.117 1.61
-
 4-(methylsulfanyl)-2-oxobutanoate pH 7.5, 25°C Zea mays
2.6.1.117 3.73
-
 4-(methylsulfanyl)-2-oxobutanoate pH 7.5, 37°C Bacillus subtilis

General Information

EC Number General Information Comment Organism
3.5.1.3 metabolism glutamine transaminase and omega-amidase potentially act in tandem to close the methionine salvage cycle in bacteria and plants Solanum lycopersicum
3.5.1.3 metabolism glutamine transaminase and omega-amidase potentially act in tandem to close the methionine salvage cycle in bacteria and plants Zea mays

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.6.1.117 0.71
-
 4-(methylsulfanyl)-2-oxobutanoate pH 7.5, 25°C Solanum lycopersicum
2.6.1.117 1.26
-
 4-(methylsulfanyl)-2-oxobutanoate pH 7.5, 37°C Bacillus subtilis
2.6.1.117 1.73
-
 4-(methylsulfanyl)-2-oxobutanoate pH 7.5, 25°C Zea mays
3.5.1.3 9.87
-
 2-Oxoglutaramate pH 8.5, 37°C Zea mays
3.5.1.3 9.98
-
 2-Oxoglutaramate pH 8.5, 37°C Zea mays
3.5.1.3 11.32
-
 2-Oxoglutaramate pH 8.5, 37°C Solanum lycopersicum