Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Van Kersavond, T.; Konopatzki, R.; Chakrabarty, S.; Blank-Landeshammer, B.; Sickmann, A.; Verhelst, S.H.L.
    Short peptides with uncleavable peptide bond mimetics as photoactivatable caspase-3 inhibitors (2019), Molecules, 24, E206.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.56 expression in Escherichia coli Mammalia

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.56 acetyl-Asp-Glu-Val-Asp(psi)(CH2NH)-Ala-NH2 no ihibition without irradiation, complete inhibition when the sample is irradiated Mammalia
3.4.22.56 hexynoyl-Asp-Glu-Val-Asp(psi)(CH2NH)-Ala-Bpa-NH2 no ihibition without irradiation, complete inhibition when the sample is irradiated Mammalia
3.4.22.56 hexynoyl-Asp-Glu-Val-Asp(psi)(CH2NH)-Ala-NH2 no ihibition without irradiation, complete inhibition when the sample is irradiated Mammalia
3.4.22.56 hexynoyl-Asp-Glu-Val-Asp(psi)(CH2NH)-Ala-photoLeu-NH2 no ihibition without irradiation, complete inhibition when the sample is irradiated Mammalia
3.4.22.56 hexynoyl-Asp-Glu-Val-Asp(triazolo)-Ala-Bpa-Gly-NH2 weak inhibition Mammalia
3.4.22.56 hexynoyl-Bpa-Asp-Glu-Val-Asp(psi)(CH2NH)-Ala-NH2 no ihibition without irradiation, complete inhibition when the sample is irradiated Mammalia
3.4.22.56 hexynoyl-photoLeu-Asp-Glu-Val-Asp(psi)(CH2NH)-Ala-NH2 no ihibition without irradiation, complete inhibition when the sample is irradiated Mammalia
3.4.22.56 additional information molecules with psi(CH2NH) or triazole uncleavable peptide bond do not show any inhibition of caspase activity without irradiation. When the samples are irradiated, the psi(CH2NH) peptides with benzophenone or diazirine photocrosslinkers show complete inhibition Mammalia

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.56 Mammalia
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.56 Ac-Asp-Glu-Val-Asp-7-amido-4-methylcoumarin + H2O
-
 Mammalia Ac-Asp-Glu-Val-Asp + 7-amino-4-methylcoumarin
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.56 37
-
 assay at Mammalia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.56 7.4
-
 assay at Mammalia