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Literature summary extracted from

  • Wang, G.; Wang, X.; Sun, L.; Gao, Y.; Niu, X.; Wang, H.
    Novel Inhibitor discovery of Staphylococcus aureus sortase B and the mechanism confirmation via molecular modeling (2018), Molecules, 23, 977 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.71 expression in Escherichia coli Staphylococcus aureus

Protein Variants

EC Number Protein Variants Comment Organism
3.4.22.71 I182A the inhibitory effect of coptisine on mutants decreases in the order wild-type > R115A > I182A > N116A Staphylococcus aureus
3.4.22.71 N116A the inhibitory effect of coptisine on mutants decreases in the order wild-type > R115A > I182A > N116A Staphylococcus aureus
3.4.22.71 R115A the inhibitory effect of coptisine on mutants decreases in the order wild-type > R115A > I182A > N116A Staphylococcus aureus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.71 coptisine coptisine can bind to the active pocket of SrtB, residues Arg115, Asn116, and Ile182 play a vital role in the interaction of SrtB with coptisine. Coptisine can reduce the adhesion of Staphylococcus aureus to human lung epithelial cells Staphylococcus aureus

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.71 Staphylococcus aureus
-
-
-
3.4.22.71 Staphylococcus aureus ATCC 29213
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.71 Dabcyl-QANPQTNEE-Edans + H2O
-
 Staphylococcus aureus Dabcyl-QANPQT + NEE-Edans
-
 ?
3.4.22.71 Dabcyl-QANPQTNEE-Edans + H2O
-
 Staphylococcus aureus ATCC 29213 Dabcyl-QANPQT + NEE-Edans
-
 ?

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.4.22.71 additional information
-
 IC50 value is 0.0087 mg/ml Staphylococcus aureus coptisine