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Literature summary extracted from

  • Saragadam, T.; Kumar, S.; Punekar, N.S.
    Characterization of 4-guanidinobutyrase from Aspergillus niger (2019), Microbiology, 165, 396-410 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.3.7 expression in Escherichia coli BL21 (DE3) Aspergillus niger

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.3.7 1,10-phenanthroline
-
 Aspergillus niger
3.5.3.7 EDTA
-
 Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.3.7 2.7
-
 4-Guanidinobutanoate pH 7.5, 37°C Aspergillus niger
3.5.3.7 53.7
-
 3-guanidinopropionate pH 7.5, 37°C Aspergillus niger

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.3.7 Zn2+ associated with Zn2+ ions Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.3.7 336000
-
 gel filtration Aspergillus niger

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.3.7 4-guanidinobutanoate + H2O Aspergillus niger
-
 4-aminobutanoate + urea
-
 ?
3.5.3.7 4-guanidinobutanoate + H2O Aspergillus niger NCIM 565
-
 4-aminobutanoate + urea
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.7 Aspergillus niger
-
-
-
3.5.3.7 Aspergillus niger NCIM 565
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.3.7
-
 Aspergillus niger

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.3.7 68
-
 pH 7.5, 37°C Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.7 3-guanidinopropionate + H2O the enzyme acts 25times more efficiently on 4-guanidinobutyrate than on 3-guanidinopropionic acid Aspergillus niger 3-aminopropionate + urea
-
 ?
3.5.3.7 3-guanidinopropionate + H2O the enzyme acts 25times more efficiently on 4-guanidinobutyrate than on 3-guanidinopropionic acid Aspergillus niger NCIM 565 3-aminopropionate + urea
-
 ?
3.5.3.7 4-guanidinobutanoate + H2O
-
 Aspergillus niger 4-aminobutanoate + urea
-
 ?
3.5.3.7 4-guanidinobutanoate + H2O the enzyme acts 25times more efficiently on 4-guanidinobutyrate than on 3-guanidinopropionic acid Aspergillus niger 4-aminobutanoate + urea
-
 ?
3.5.3.7 4-guanidinobutanoate + H2O
-
 Aspergillus niger NCIM 565 4-aminobutanoate + urea
-
 ?
3.5.3.7 4-guanidinobutanoate + H2O the enzyme acts 25times more efficiently on 4-guanidinobutyrate than on 3-guanidinopropionic acid Aspergillus niger NCIM 565 4-aminobutanoate + urea
-
 ?
3.5.3.7 additional information other guanidinium compounds, namely L-arginine, agmatine, 2-guanidinoacetic acid, D-arginine and L-homoarginine, are not substrates for this enzyme Aspergillus niger ?
-
 ?
3.5.3.7 additional information other guanidinium compounds, namely L-arginine, agmatine, 2-guanidinoacetic acid, D-arginine and L-homoarginine, are not substrates for this enzyme Aspergillus niger NCIM 565 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.3.7 homohexamer 6 * 50400, SDS-PAGE Aspergillus niger

Synonyms

EC Number Synonyms Comment Organism
3.5.3.7 GBase
-
 Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.3.7 7.5
-
-
 Aspergillus niger

Expression

EC Number Organism Comment Expression
3.5.3.7 Aspergillus niger the enzyme is strictly inducible by agmatine or 4-guanidinobutanoate up

General Information

EC Number General Information Comment Organism
3.5.3.7 metabolism the enzyme is involved in agmatine catabolism. While 4-guanidinobutanoate is an efficient nitrogen source, Aspergillus niger grows very poorly on 3-guanidinopropionate Aspergillus niger