EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.24 | gene CERS5, recombinant expression in HEK-293T cells, quantitative real-time quantitative PCR enzyme expression analysis | Homo sapiens |
2.3.1.24 | gene CERS6, recombinant expression in HEK-293T cells, quantitative real-time quantitative PCR enzyme expression analysis | Homo sapiens |
2.3.1.297 | gene CERS2, recombinant expression in HEK-293T cells, quantitative real-time quantitative PCR enzyme expression analysis | Homo sapiens |
2.3.1.297 | gene CERS4, recombinant expression in HEK-293T cells, quantitative real-time quantitative PCR enzyme expression analysis | Homo sapiens |
2.3.1.298 | expressed in HEK-293T cells | Homo sapiens |
2.3.1.298 | gene CERS3, recombinant expression in HEK-293T cells, quantitative real-time quantitative PCR enzyme expression analysis | Homo sapiens |
2.3.1.299 | gene CERS1, recombinant expression in HEK-293T cells, quantitative real-time quantitative PCR enzyme expression analysis | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.1.24 | additional information | knockdown of CerS6 by siRNA. Profiles of non-hydroxylated and 2-hydroxy-ceramides in transgenic HeLa cells expressing different CerS isozymes and or different specific interfence RNAs, overview | Homo sapiens |
2.3.1.24 | additional information | profiles of non-hydroxylated and 2-hydroxy-ceramides in transgenic HeLa cells expressing different CerS isozymes and or different specific interfence RNAs, overview | Homo sapiens |
2.3.1.297 | additional information | in HeLa cells overproducing the fatty acid 2-hydroxylase FA2H, knockdown of CerS2 by siRNA results in a reduction in total long-chain 2-hydroxy-ceramides, confirming enzyme substrate specificity for chain length. Profiles of non-hydroxylated and 2-hydroxy-ceramides in transgenic HeLa cells expressing different CerS isozymes and or different specific interfence RNAs, overview | Homo sapiens |
2.3.1.297 | additional information | profiles of non-hydroxylated and 2-hydroxy-ceramides in transgenic HeLa cells expressing different CerS isozymes and or different specific interfence RNAs, overview | Homo sapiens |
2.3.1.298 | additional information | profiles of non-hydroxylated and 2-hydroxy-ceramides in transgenic HeLa cells expressing different CerS isozymes and or different specific interfence RNAs, overview | Homo sapiens |
2.3.1.299 | additional information | profiles of non-hydroxylated and 2-hydroxy-ceramides in transgenic HeLa cells expressing different CerS isozymes and or different specific interfence RNAs, overview | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.298 | fumonisin B1 | - |
Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.24 | additional information | Homo sapiens | CerS6 lysate produces nonhydroxy-dihydro-ceramides that included strong bands for C14:0- and C16:0-dihydro-ceramide, but a weak band for C18:0-dihydro-ceramide | ? | - |
? | |
2.3.1.24 | sphingosine + palmitoyl-CoA | Homo sapiens | - |
N-palmitoylsphingosine + CoA | - |
? | |
2.3.1.297 | sphingosine + behenoyl-CoA | Homo sapiens | - |
N-behenoylsphingosine + CoA | - |
? | |
2.3.1.297 | sphingosine + eicosanoyl-CoA | Homo sapiens | - |
N-eicosanoylsphingosine + CoA | - |
? | |
2.3.1.297 | sphingosine + lignoceroyl-CoA | Homo sapiens | - |
N-lignoceroylsphingosine + CoA | - |
? | |
2.3.1.298 | an ultra-long-chain fatty acyl-CoA + a sphingoid base | Homo sapiens | - |
an ultra-long-chain ceramide + CoA | - |
? | |
2.3.1.298 | sphingosine + behenoyl-CoA | Homo sapiens | - |
N-behenoylsphingosine + CoA | - |
? | |
2.3.1.298 | sphingosine + eicosanoyl-CoA | Homo sapiens | - |
N-eicosanoylsphingosine + CoA | - |
? | |
2.3.1.298 | sphingosine + lignoceroyl-CoA | Homo sapiens | - |
N-lignoceroylsphingosine + CoA | - |
? | |
2.3.1.298 | sphingosine + palmitoyl-CoA | Homo sapiens | - |
N-palmitoylsphingosine + CoA | - |
? | |
2.3.1.298 | sphingosine + stearoyl-CoA | Homo sapiens | - |
N-stearoylsphingosine + CoA | - |
? | |
2.3.1.299 | dihydrosphingosine + 2-hydroxystearoyl-CoA | Homo sapiens | - |
N-2-hydroxystearoylsphingosine + CoA | - |
? | |
2.3.1.299 | dihydrosphingosine + stearoyl-CoA | Homo sapiens | - |
N-stearoyldihydrosphingosine + CoA | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.24 | Homo sapiens | Q6ZMG9 | - |
- |
2.3.1.24 | Homo sapiens | Q8N5B7 | - |
- |
2.3.1.297 | Homo sapiens | Q96G23 | - |
- |
2.3.1.297 | Homo sapiens | Q9HA82 | - |
- |
2.3.1.298 | Homo sapiens | - |
- |
- |
2.3.1.298 | Homo sapiens | Q8IU89 | - |
- |
2.3.1.299 | Homo sapiens | P27544 | - |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.3.1.24 | keratinocyte | epidermal, expression analysis of the CerS family members during keratinocyte differentiation, overview | Homo sapiens | - |
2.3.1.297 | keratinocyte | epidermal, expression analysis of the CerS family members during keratinocyte differentiation, overview. A slight increase in CerS4 mRNA is observed at the late stage of differentiation | Homo sapiens | - |
2.3.1.297 | keratinocyte | epidermal, expression analysis of the CerS family members during keratinocyte differentiation, overview. Expression of CerS2 mRNA remains nearly unchanged during differentiation | Homo sapiens | - |
2.3.1.298 | epidermis | - |
Homo sapiens | - |
2.3.1.298 | keratinocyte | - |
Homo sapiens | - |
2.3.1.298 | keratinocyte | epidermal, expression analysis of the CerS family members during keratinocyte differentiation, overview. Upon differentiation, CerS3 mRNA expression significantly increases and remains high throughout differentiation | Homo sapiens | - |
2.3.1.299 | keratinocyte | - |
Homo sapiens | - |
2.3.1.299 | additional information | expression analysis of the CerS family members during epidermal keratinocyte differentiation, overview. For all CerS family members except CerS1, mRNA expression is detected in undifferentiated keratinocytes | Homo sapiens | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.24 | additional information | substrate specificity, overview | Homo sapiens | ? | - |
? | |
2.3.1.24 | additional information | CerS6 lysate produces nonhydroxy-dihydro-ceramides that included strong bands for C14:0- and C16:0-dihydro-ceramide, but a weak band for C18:0-dihydro-ceramide | Homo sapiens | ? | - |
? | |
2.3.1.24 | sphingosine + 2-hydroxy-palmitoyl-CoA | - |
Homo sapiens | N-2-hydroxy-palmitoyl-sphingosine + CoA | - |
? | |
2.3.1.24 | sphingosine + palmitoyl-CoA | - |
Homo sapiens | N-palmitoylsphingosine + CoA | - |
? | |
2.3.1.297 | additional information | substrate specificity, overview | Homo sapiens | ? | - |
? | |
2.3.1.297 | additional information | substrate specificity, overview. Long-chain preference of CerS2 | Homo sapiens | ? | - |
? | |
2.3.1.297 | sphingosine + 2-hydroxybehenoyl-CoA | - |
Homo sapiens | N-2-hydroxybehenoylsphingosine + CoA | - |
? | |
2.3.1.297 | sphingosine + 2-hydroxyeicosanoyl-CoA | - |
Homo sapiens | N-2-hydroxyeicosanoylsphingosine + CoA | - |
? | |
2.3.1.297 | sphingosine + 2-hydroxylignoceroyl-CoA | - |
Homo sapiens | N-2-hydroxylignoceroylsphingosine + CoA | - |
? | |
2.3.1.297 | sphingosine + behenoyl-CoA | - |
Homo sapiens | N-behenoylsphingosine + CoA | - |
? | |
2.3.1.297 | sphingosine + eicosanoyl-CoA | - |
Homo sapiens | N-eicosanoylsphingosine + CoA | - |
? | |
2.3.1.297 | sphingosine + lignoceroyl-CoA | - |
Homo sapiens | N-lignoceroylsphingosine + CoA | - |
? | |
2.3.1.298 | an ultra-long-chain fatty acyl-CoA + a sphingoid base | - |
Homo sapiens | an ultra-long-chain ceramide + CoA | - |
? | |
2.3.1.298 | behenoyl-CoA + dihydrosphingosine | - |
Homo sapiens | CoA + N-behenoyldihydrosphingosine | - |
? | |
2.3.1.298 | lignoceroyl-CoA + dihydrosphingosine | highest activity | Homo sapiens | CoA + N-lignoceroyldihydrosphingosine | - |
? | |
2.3.1.298 | additional information | isozyme CerS3 exhibits broad substrate specificity toward medium- to long-chain fatty acyl-CoAs (short-chain having less than C18, medium-chain with C18-C22, and long-chain with more than C22). In HEK-293T cells overproducing both CerS3 and 4-hydroxysphinganine ceramide fatty acyl 2-hydroxylase (FA2H), no 2-hydroxy-C16:0-ceramide is detected, but both medium- and long-chain 2-hydroxy-ceramides. CerS3 may use both medium- and long-chain 2-hydroxy-fatty acyl-CoAs as substrates | Homo sapiens | ? | - |
? | |
2.3.1.298 | additional information | the enzyme can also use both medium- and long-chain 2-hydroxy-fatty acyl-CoAs as substrates | Homo sapiens | ? | - |
? | |
2.3.1.298 | sphingosine + 2-hydroxybehenoyl-CoA | - |
Homo sapiens | N-2-hydroxybehenoylsphingosine + CoA | - |
? | |
2.3.1.298 | sphingosine + 2-hydroxyeicosanoyl-CoA | - |
Homo sapiens | N-2-hydroxyeicosanoylsphingosine + CoA | - |
? | |
2.3.1.298 | sphingosine + 2-hydroxylignoceroyl-CoA | - |
Homo sapiens | N-2-hydroxylignoceroylsphingosine + CoA | - |
? | |
2.3.1.298 | sphingosine + 2-hydroxypalmitoyl-CoA | - |
Homo sapiens | N-2-hydroxypalmitoylsphingosine + CoA | - |
? | |
2.3.1.298 | sphingosine + 2-hydroxystearoyl-CoA | - |
Homo sapiens | N-2-hydroxystearoylsphingosine + CoA | - |
? | |
2.3.1.298 | sphingosine + behenoyl-CoA | - |
Homo sapiens | N-behenoylsphingosine + CoA | - |
? | |
2.3.1.298 | sphingosine + eicosanoyl-CoA | - |
Homo sapiens | N-eicosanoylsphingosine + CoA | - |
? | |
2.3.1.298 | sphingosine + lignoceroyl-CoA | - |
Homo sapiens | N-lignoceroylsphingosine + CoA | - |
? | |
2.3.1.298 | sphingosine + palmitoyl-CoA | - |
Homo sapiens | N-palmitoylsphingosine + CoA | - |
? | |
2.3.1.298 | sphingosine + stearoyl-CoA | - |
Homo sapiens | N-stearoylsphingosine + CoA | - |
? | |
2.3.1.298 | stearoyl-CoA + dihydrosphingosine | - |
Homo sapiens | CoA + N-stearoyldihydrosphingosine | - |
? | |
2.3.1.299 | dihydrosphingosine + 2-hydroxystearoyl-CoA | - |
Homo sapiens | N-2-hydroxystearoylsphingosine + CoA | - |
? | |
2.3.1.299 | dihydrosphingosine + stearoyl-CoA | - |
Homo sapiens | N-stearoyldihydrosphingosine + CoA | - |
? | |
2.3.1.299 | additional information | substrate specificity, overview. CerS1 can use C18:0-CoA but not C18:1-CoA as a substrate | Homo sapiens | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.24 | ceramide synthase 5 | - |
Homo sapiens |
2.3.1.24 | ceramide synthase 6 | - |
Homo sapiens |
2.3.1.24 | CerS5 | - |
Homo sapiens |
2.3.1.24 | CerS6 | - |
Homo sapiens |
2.3.1.24 | LASS5 | - |
Homo sapiens |
2.3.1.24 | Lass6 | - |
Homo sapiens |
2.3.1.24 | longevity assurance homologue 5 | - |
Homo sapiens |
2.3.1.24 | longevity assurance homologue 6 | - |
Homo sapiens |
2.3.1.297 | ceramide synthase 2 | - |
Homo sapiens |
2.3.1.297 | ceramide synthase 4 | - |
Homo sapiens |
2.3.1.297 | CerS2 | - |
Homo sapiens |
2.3.1.297 | CerS4 | - |
Homo sapiens |
2.3.1.297 | Lass2 | - |
Homo sapiens |
2.3.1.297 | Lass4 | - |
Homo sapiens |
2.3.1.297 | longevity assurance homologue 2 | - |
Homo sapiens |
2.3.1.297 | longevity assurance homologue 4 | - |
Homo sapiens |
2.3.1.298 | ceramide synthase 3 | - |
Homo sapiens |
2.3.1.298 | CerS3 | - |
Homo sapiens |
2.3.1.298 | LASS3 | - |
Homo sapiens |
2.3.1.298 | longevity assurance homologue 3 | - |
Homo sapiens |
2.3.1.299 | ceramide synthase 1 | - |
Homo sapiens |
2.3.1.299 | CerS1 | - |
Homo sapiens |
2.3.1.299 | LASS1 | - |
Homo sapiens |
2.3.1.299 | longevity assurance homologue 1 | - |
Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.1.24 | 37 | - |
assay at | Homo sapiens |
2.3.1.297 | 37 | - |
assay at | Homo sapiens |
2.3.1.298 | 37 | - |
assay at | Homo sapiens |
2.3.1.299 | 37 | - |
assay at | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.24 | 7.5 | - |
assay at | Homo sapiens |
2.3.1.297 | 7.5 | - |
assay at | Homo sapiens |
2.3.1.298 | 7.5 | - |
assay at | Homo sapiens |
2.3.1.299 | 7.5 | - |
assay at | Homo sapiens |
EC Number | Organism | Comment | Expression |
---|---|---|---|
2.3.1.298 | Homo sapiens | enzyme mRNA expression significantly increases and remains high throughout keratinocyte differentiation | up |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.3.1.24 | malfunction | profiles of non-hydroxylated and 2-hydroxy-ceramides in transgenic HeLa cells expressing different CerS isozymes and or different specific interfence RNAs, overview | Homo sapiens |
2.3.1.24 | physiological function | differences in the expression patterns of CerS family members may play an important role in the production of the CER/2-hydroxy ceramide (CER) compositions of different chain lengths observed in different cell types and even in the altered production that occurring during keratinocyte differentiation | Homo sapiens |
2.3.1.297 | malfunction | in HeLa cells overproducing the fatty acid 2-hydroxylase FA2H, knockdown of CerS2 results in a reduction in total long-chain 2-hydroxy-ceramides, confirming enzyme substrate specificity for chain length. Profiles of non-hydroxylated and 2-hydroxy-ceramides in transgenic HeLa cells expressing different CerS isozymes and or different specific interfence RNAs, overview | Homo sapiens |
2.3.1.297 | malfunction | profiles of non-hydroxylated and 2-hydroxy-ceramides in transgenic HeLa cells expressing different CerS isozymes and or different specific interfence RNAs, overview | Homo sapiens |
2.3.1.297 | physiological function | differences in the expression patterns of CerS family members may play an important role in the production of the CER/2-hydroxy ceramide (CER) compositions of different chain lengths observed in different cell types and even in the altered production that occurring during keratinocyte differentiation | Homo sapiens |
2.3.1.298 | malfunction | profiles of non-hydroxylated and 2-hydroxy-ceramides in transgenic HeLa cells expressing different CerS isozymes and or different specific interfence RNAs, overview | Homo sapiens |
2.3.1.298 | physiological function | isozyme CerS3 may be important in ceramide and 2-hydroxy-ceramide synthesis in epidermis. Differences in the expression patterns of CerS family members may play an important role in the production of the CER/2-hydroxy ceramide (CER) compositions of different chain lengths observed in different cell types and even in the altered production that occurring during keratinocyte differentiation | Homo sapiens |
2.3.1.299 | malfunction | profiles of non-hydroxylated and 2-hydroxy-ceramides in transgenic HeLa cells expressing different CerS isozymes and or different specific interfence RNAs, overview | Homo sapiens |
2.3.1.299 | physiological function | differences in the expression patterns of CerS family members may play an important role in the production of the CER/2-hydroxy ceramide (CER) compositions of different chain lengths observed in different cell types and even in the altered production that occurring during keratinocyte differentiation | Homo sapiens |