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Literature summary extracted from

  • Laviad, E.L.; Albee, L.; Pankova-Kholmyansky, I.; Epstein, S.; Park, H.; Merrill, A.H.; Futerman, A.H.
    Characterization of ceramide synthase 2 tissue distribution, substrate specificity, and inhibition by sphingosine 1-phosphate (2008), J. Biol. Chem., 283, 5677-5684 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.297 gene CERS2, quantitative real-time PCR expression analysis, recombinant expression of C-terminally HA-tagged CerS2 in the endoplasmic reticulum of yeast cells, recombinant expression of FLAG-tagged CerS2 in HEK-293T cells Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.297 additional information CerS2 knockdown by siRNA Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.297 sphingosine 1-phosphate S1P, noncompetitive inhibition. Bioactive sphingolipid sphingosine 1-phosphate has a regulatory function for CerS2. Residues Arg230 and Arg325 are essential for S1P binding to the S1P1 receptor. Mutation of both residues to alanine completely abolishes the inhibitory effect of S1P on CerS2 without affecting the basal activity of CerS2. Thus, S1P can regulate CerS2 activity via a direct interaction with an S1P receptor-like motif found uniquely in CerS2 Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.297 additional information
-
 additional information Michaelis-Menten kinetics Mus musculus
2.3.1.297 0.0025
-
 D-erythro-sphinganine pH 7.5, 37°C, recombinant enzyme Mus musculus
2.3.1.297 0.0032
-
 D-erythro-sphinganine pH 7.5, 37°C, recombinant enzyme, in presence of inhibitor sphingosine 1-phosphate Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.297 endoplasmic reticulum
-
 Mus musculus 5783
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.297 D-erythro-sphinganine + arachidoyl-CoA Mus musculus
-
 N-arachidoyl-D-sphinganine + CoA
-
 ?
2.3.1.297 D-erythro-sphinganine + arachidoyl-CoA Mus musculus C57BL/6
-
 N-arachidoyl-D-sphinganine + CoA
-
 ?
2.3.1.297 D-erythro-sphinganine + behenoyl-CoA Mus musculus
-
 N-behenoyl-D-sphinganine + CoA
-
 ?
2.3.1.297 D-erythro-sphinganine + behenoyl-CoA Mus musculus C57BL/6
-
 N-behenoyl-D-sphinganine + CoA
-
 ?
2.3.1.297 D-erythro-sphinganine + cerotoyl-CoA Mus musculus
-
 N-cerotoyl-D-sphinganine + CoA
-
 ?
2.3.1.297 D-erythro-sphinganine + cerotoyl-CoA Mus musculus C57BL/6
-
 N-cerotoyl-D-sphinganine + CoA
-
 ?
2.3.1.297 D-erythro-sphinganine + lignoceroyl-CoA Mus musculus
-
 N-lignoceroyl-D-sphinganine + CoA
-
 ?
2.3.1.297 D-erythro-sphinganine + nervonoyl-CoA Mus musculus
-
 N-nervonoyl-D-sphinganine + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.297 Mus musculus Q924Z4
-
-
2.3.1.297 Mus musculus C57BL/6 Q924Z4
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.297 brain low expression level of CerS2 Mus musculus
-
2.3.1.297 kidney high expression level of CerS2 Mus musculus
-
2.3.1.297 liver high expression level of CerS2 Mus musculus
-
2.3.1.297 additional information CerS2 tissue expression analysis, comparisons with the other CerS isozymes (CerS1, 3, 4-6). CerS2 mRNA is widely distributed and found at high levels in various tissues Mus musculus
-
2.3.1.297 skeletal muscle low expression level of CerS2 Mus musculus
-
2.3.1.297 skin low expression level of CerS2 Mus musculus
-
2.3.1.297 testis low expression level of CerS2 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.297 D-erythro-sphinganine + arachidoyl-CoA
-
 Mus musculus N-arachidoyl-D-sphinganine + CoA
-
 ?
2.3.1.297 D-erythro-sphinganine + arachidoyl-CoA
-
 Mus musculus C57BL/6 N-arachidoyl-D-sphinganine + CoA
-
 ?
2.3.1.297 D-erythro-sphinganine + behenoyl-CoA
-
 Mus musculus N-behenoyl-D-sphinganine + CoA
-
 ?
2.3.1.297 D-erythro-sphinganine + behenoyl-CoA
-
 Mus musculus C57BL/6 N-behenoyl-D-sphinganine + CoA
-
 ?
2.3.1.297 D-erythro-sphinganine + cerotoyl-CoA
-
 Mus musculus N-cerotoyl-D-sphinganine + CoA
-
 ?
2.3.1.297 D-erythro-sphinganine + cerotoyl-CoA
-
 Mus musculus C57BL/6 N-cerotoyl-D-sphinganine + CoA
-
 ?
2.3.1.297 D-erythro-sphinganine + lignoceroyl-CoA
-
 Mus musculus N-lignoceroyl-D-sphinganine + CoA
-
 ?
2.3.1.297 D-erythro-sphinganine + nervonoyl-CoA
-
 Mus musculus N-nervonoyl-D-sphinganine + CoA
-
 ?
2.3.1.297 additional information CerS2 has a remarkable acyl-CoA specificity, it uses a wider range of acyl-CoAs, synthesizing ceramides containing C20:0, C22:0, C24:1, C24:0, C26:1, and C26:0 fatty acids, but does not synthesize ceramides containing C16:0 fatty acids and synthesizes only low, and statistically insignificant levels of C18:0-ceramide, overview Mus musculus ?
-
 ?
2.3.1.297 additional information CerS2 has a remarkable acyl-CoA specificity, it uses a wider range of acyl-CoAs, synthesizing ceramides containing C20:0, C22:0, C24:1, C24:0, C26:1, and C26:0 fatty acids, but does not synthesize ceramides containing C16:0 fatty acids and synthesizes only low, and statistically insignificant levels of C18:0-ceramide, overview Mus musculus C57BL/6 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.297 ceramide synthase 2
-
 Mus musculus
2.3.1.297 CerS2
-
 Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.297 37
-
 assay at Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.297 7.5
-
 assay at Mus musculus

General Information

EC Number General Information Comment Organism
2.3.1.297 physiological function good correlation between CerS2 mRNA levels and levels of ceramide and sphingomyelin containing long acyl chains in tissues with CerS2 mRNA expression at high levels. The activity of CerS2 can be regulated by another bioactive sphingolipid, sphingosine 1-phosphate (S1P), via interaction of S1P with two residues that are part of an S1P receptor-like motif found only in CerS2 Mus musculus