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Literature summary extracted from

  • Pauwels, F.; Vergauwen, B.; Vanrobaeys, F.; Devreese, B.; Van Beeumen, J.J.
    Purification and characterization of a chimeric enzyme from Haemophilus influenzae Rd that exhibits glutathione-dependent peroxidase activity (2003), J. Biol. Chem., 278, 16658-16666 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.27 expression in Escherichia coli B834(DE3) Haemophilus influenzae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.27 0.00229
-
 H2O2 pH 7.8, 25°C Haemophilus influenzae
1.11.1.27 0.2088
-
 tert-butyl hydroperoxide pH 7.8, 25°C Haemophilus influenzae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.27 26915
-
 nonreduced monomeric enzyme form, electrospray ionization mass spectrometry Haemophilus influenzae
1.11.1.27 53216
-
 oxidized dimeric enzyme form, electrospray ionization mass spectrometry Haemophilus influenzae

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.27 Haemophilus influenzae P44758
-
-
1.11.1.27 Haemophilus influenzae ATCC 51907 P44758
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.27
-
 Haemophilus influenzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.27 2 glutathione + H2O2
-
 Haemophilus influenzae glutathione disulfide + 2 H2O
-
 ?
1.11.1.27 2 glutathione + H2O2
-
 Haemophilus influenzae ATCC 51907 glutathione disulfide + 2 H2O
-
 ?
1.11.1.27 2 glutathione + tert-butyl hydroperoxide
-
 Haemophilus influenzae glutathione disulfide + H2O + tert-butyl alcohol
-
 ?
1.11.1.27 2 glutathione + tert-butyl hydroperoxide
-
 Haemophilus influenzae ATCC 51907 glutathione disulfide + H2O + tert-butyl alcohol
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.27 dimer 2 * 26915, oxidized dimeric enzyme form, electrospray ionization mass spectrometry Haemophilus influenzae
1.11.1.27 monomer 1 * 26915, nonreduced monomeric enzyme form, electrospray ionization mass spectrometry Haemophilus influenzae

Synonyms

EC Number Synonyms Comment Organism
1.11.1.27 HI0572
-
 Haemophilus influenzae
1.11.1.27 PGdx
-
 Haemophilus influenzae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.11.1.27 56.7
-
 tert-butyl hydroperoxide pH 7.8, 25°C Haemophilus influenzae
1.11.1.27 5010
-
 H2O2 pH 7.8, 25°C Haemophilus influenzae