Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Merrill, A.H.; van Echten, G.; Wang, E.; Sandhoff, K.
    Fumonisin B1 inhibits sphingosine (sphinganine) N-acyltransferase and de novo sphingolipid biosynthesis in cultured neurons in situ (1993), J. Biol. Chem., 268, 27299-27306 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.24 fumonisin B1 reversible competitive inhibition, inhibits ceramide synthase in mouse brain microsomes with a competitive-like kinetic behavior with respect to both sphinganine and stearoyl-CoA. Fumonisin B1 inhibits ceramide synthase activity when palmitoyl-CoA, stearoyl-CoA, or lignoceroyl-CoA are used as the co-substrate, cf. EC 2.3.1.297 and 2.3.1.24 Mus musculus
2.3.1.24 additional information fumonisins block sphingosine biosynthesis by inhibiting the conversion of sphinganine to dihydroceramides, which precedes introduction of the 4,5-trans-double bond of sphingosine. Fumonisins, mycotoxins produced by Fusarium moniliforme and a number of other fungi, cause neuronal degeneration, liver and renal toxicity, cancer, and other injury to animals. Fumonisin Bl inhibits complex sphirqolipid synthesis from galactose and sphinganine, effects on the amounts of free sphingosine and sphinganine and on total complex sphingolipids, overview Mus musculus
2.3.1.297 fumonisin B1 reversible competitive inhibition, inhibits ceramide synthase in mouse brain microsomes with a competitive-like kinetic behavior with respect to both sphinganine and stearoyl-CoA. Fumonisin B1 inhibits ceramide synthase activity when palmitoyl-CoA, stearoyl-CoA, or lignoceroyl-CoA are used as the co-substrate, cf. EC 2.3.1.297 and 2.3.1.24 Mus musculus
2.3.1.297 additional information fumonisins block sphingosine biosynthesis by inhibiting the conversion of sphinganine to dihydroceramides, which precedes introduction of the 4,5-trans-double bond of sphingosine. Fumonisins, mycotoxins produced by Fusarium moniliforme and a number of other fungi, cause neuronal degeneration, liver and renal toxicity, cancer, and other injury to animals. Fumonisin Bl inhibits complex sphirqolipid synthesis from galactose and sphinganine, effects on the amounts of free sphingosine and sphinganine and on total complex sphingolipids, overview Mus musculus
2.3.1.299 fumonisin B1 reversible competitive inhibition, inhibits ceramide synthase in mouse brain microsomes with a competitive-like kinetic behavior with respect to both sphinganine and stearoyl-CoA. Fumonisin B1 inhibits ceramide synthase activity when palmitoyl-CoA, stearoyl-CoA, or lignoceroyl-CoA are used as the co-substrate, cf. EC 2.3.1.297 and 2.3.1.24 Mus musculus
2.3.1.299 additional information fumonisins block sphingosine biosynthesis by inhibiting the conversion of sphinganine to dihydroceramides, which precedes introduction of the 4,5-trans-double bond of sphingosine. Fumonisins, mycotoxins produced by Fusarium moniliforme and a number of other fungi, cause neuronal degeneration, liver and renal toxicity, cancer, and other injury to animals. Fumonisin Bl inhibits complex sphirqolipid synthesis from galactose and sphinganine, effects on the amounts of free sphingosine and sphinganine and on total complex sphingolipids, overview Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.299 0.003
-
 stearoyl-CoA pH 7.4, 25°C Mus musculus
2.3.1.299 0.035
-
 D-erythro-sphinganine pH 7.4, 25°C Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.24 microsome
-
 Mus musculus
-
-
2.3.1.297 microsome
-
 Mus musculus
-
-
2.3.1.299 microsome
-
 Mus musculus
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.297 D-erythro-sphinganine + lignoceroyl-CoA Mus musculus
-
 N-lignoceroyl-D-sphinganine + CoA
-
 ?
2.3.1.297 D-erythro-sphinganine + lignoceroyl-CoA Mus musculus NMRI
-
 N-lignoceroyl-D-sphinganine + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.24 Mus musculus
-
-
-
2.3.1.24 Mus musculus NMRI
-
-
-
2.3.1.297 Mus musculus
-
-
-
2.3.1.297 Mus musculus NMRI
-
-
-
2.3.1.299 Mus musculus
-
-
-
2.3.1.299 Mus musculus NMRI
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.24 brain
-
 Mus musculus
-
2.3.1.24 cerebellum
-
 Mus musculus
-
2.3.1.24 granule cell
-
 Mus musculus
-
2.3.1.24 neuron cerebellar Mus musculus
-
2.3.1.297 brain
-
 Mus musculus
-
2.3.1.297 cerebellum
-
 Mus musculus
-
2.3.1.297 granule cell
-
 Mus musculus
-
2.3.1.297 neuron cerebellar Mus musculus
-
2.3.1.299 brain
-
 Mus musculus
-
2.3.1.299 cerebellum
-
 Mus musculus
-
2.3.1.299 granule cell
-
 Mus musculus
-
2.3.1.299 neuron cerebellar Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.24 D-erythro-sphinganine + palmitoyl-CoA low activity Mus musculus N-palmitoyl-D-sphinganine
-
 ?
2.3.1.24 D-erythro-sphinganine + palmitoyl-CoA low activity Mus musculus NMRI N-palmitoyl-D-sphinganine
-
 ?
2.3.1.297 D-erythro-sphinganine + lignoceroyl-CoA
-
 Mus musculus N-lignoceroyl-D-sphinganine + CoA
-
 ?
2.3.1.297 D-erythro-sphinganine + lignoceroyl-CoA
-
 Mus musculus NMRI N-lignoceroyl-D-sphinganine + CoA
-
 ?
2.3.1.299 D-erythro-sphinganine + stearoyl-CoA
-
 Mus musculus N-stearoyl-D-sphinganine + CoA
-
 ?
2.3.1.299 D-erythro-sphinganine + stearoyl-CoA
-
 Mus musculus NMRI N-stearoyl-D-sphinganine + CoA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.24 ceramide synthase
-
 Mus musculus
2.3.1.24 More see also EC 2.3.1.299 and EC 2.3.1.297 Mus musculus
2.3.1.297 ceramide synthase
-
 Mus musculus
2.3.1.297 More see also EC 2.3.1.24 and EC 2.3.1.299 Mus musculus
2.3.1.299 ceramide synthase
-
 Mus musculus
2.3.1.299 More see also EC 2.3.1.24 and EC 2.3.1.297 Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.299 25
-
 assay at Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.299 7.4
-
 assay at Mus musculus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.1.24 additional information
-
 additional information inhibition kinetics Mus musculus
2.3.1.297 additional information
-
 additional information inhibition kinetics Mus musculus
2.3.1.299 additional information
-
 additional information inhibition kinetics Mus musculus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.3.1.299 0.000075
-
 pH 7.4, 25°C Mus musculus fumonisin B1