Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Mandon, E.C.; Ehses, I.; Rother, J.; van Echten, G.; Sandhoff, K.
    Subcellular localization and membrane topology of serine palmitoyltransferase, 3-dehydrosphinganine reductase, and sphinganine N-acyltransferase in mouse liver (1992), J. Biol. Chem., 267, 11144-11148 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.24 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid
-
 Mus musculus
2.3.1.24 additional information enzyme activitiy does not change by pretreating the endoplasmic reticulum-derived membrane vesicles with detergent Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.24 endoplasmic reticulum mainly, the enzyme faces the cytosolic side of the endoplasmic reticulum, so that substrates have free access to the active site Mus musculus 5783
-
2.3.1.24 Golgi membrane low enzyme level Mus musculus 139
-
2.3.1.24 vesicle membrane vesicles derived from endoplasmic reticlum and Golgi membranes, mouse liver smooth and rough ER vesicles Mus musculus 31982
-

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.24 Mus musculus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.24 liver
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.24 0.000049
-
 endoplasmic reticulum-derived membrane vesicles, pH 7.5, 37°C Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.24 3-dehydrosphinganine + palmitoyl-CoA
-
 Mus musculus N-palmitoyl-3-dehydrosphinganine
-
 ?
2.3.1.24 sphinganine + palmitoyl-CoA
-
 Mus musculus N-palmitoylsphinganine + CoA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.24 SpAT
-
 Mus musculus
2.3.1.24 sphinganine N-acyltransferase
-
 Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.24 37
-
 assay at Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.24 7.2
-
 assay at Mus musculus

General Information

EC Number General Information Comment Organism
2.3.1.24 metabolism serine palmitoyltransferase, 3-dehydrosphinganine reductase, and sphinganine N-acyltransferase are responsible for the first steps in sphingolipid biosynthesis forming 3-oxosphinganine, sphinganine, and dihydroceramide, respectively Mus musculus