Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Lin, H.; Fischbach, M.; Liu, D.; Walsh, C.
    In vitro characterization of salmochelin and enterobactin trilactone hydrolases IroD, IroE, and Fes (2005), J. Am. Chem. Soc., 127, 11075-11084 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.107 expressed in Escherichia coli BL21(DE3) cells Escherichia coli
3.1.1.108 expressed in Escherichia coli BL21(DE3) cells Escherichia coli
3.1.1.109 expressed in Escherichia coli BL21(DE3) cells Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.107 0.0034
-
 ferric enterobactin at pH 7.5 and 23°C Escherichia coli
3.1.1.107 0.0046
-
 ferric diglucosyl-enterobactin at pH 7.5 and 23°C Escherichia coli
3.1.1.107 0.0048
-
 ferric monoglucosyl-enterobactin at pH 7.5 and 23°C Escherichia coli
3.1.1.107 0.016
-
 enterobactin at pH 7.5 and 23°C Escherichia coli
3.1.1.107 0.016
-
 ferric triglucosyl-enterobactin Km above 0.016 mM, at pH 7.5 and 23°C Escherichia coli
3.1.1.107 0.029
-
 monoglucosyl-enterobactin at pH 7.5 and 23°C Escherichia coli
3.1.1.107 0.039
-
 diglucosyl-enterobactin at pH 7.5 and 23°C Escherichia coli
3.1.1.107 0.155
-
 triglucosyl-enterobactin at pH 7.5 and 23°C Escherichia coli
3.1.1.108 0.00029
-
 iron(III)-monoglucosyl-enterobactin at pH 7.5 and 23°C Escherichia coli
3.1.1.108 0.001
-
 iron(III)-enterobactin at pH 7.5 and 23°C Escherichia coli
3.1.1.108 0.1
-
 monoglucosyl-enterobactin at pH 7.5 and 23°C Escherichia coli
3.1.1.108 0.13
-
 enterobactin at pH 7.5 and 23°C Escherichia coli
3.1.1.108 0.13
-
 diglucosyl-enterobactin at pH 7.5 and 23°C Escherichia coli
3.1.1.109 0.00008
-
 iron(III)-[monoglucosyl-enterobactin] complex at pH 7.5 and 23°C Escherichia coli
3.1.1.109 0.00012
-
 iron(III)-enterobactin at pH 7.5 and 23°C Escherichia coli
3.1.1.109 0.00012
-
 iron(III)-[diglucosyl-enterobactin] complex at pH 7.5 and 23°C Escherichia coli
3.1.1.109 0.00043
-
 iron(III)-[triglucosyl-enterobactin] complex at pH 7.5 and 23°C Escherichia coli
3.1.1.109 0.04
-
 enterobactin at pH 7.5 and 23°C Escherichia coli
3.1.1.109 0.06
-
 [monoglucosyl-enterobactin] complex at pH 7.5 and 23°C Escherichia coli
3.1.1.109 0.12
-
 [diglucosyl-enterobactin] complex at pH 7.5 and 23°C Escherichia coli
3.1.1.109 0.16
-
 [triglucosyl-enterobactin] complex at pH 7.5 and 23°C Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.107 periplasm
-
 Escherichia coli
-
-
3.1.1.109 cytoplasm
-
 Escherichia coli 5737
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.107 diglucosyl-enterobactin + H2O Escherichia coli
-
 diglucosyl-(2,3-dihydroxybenzoylserine)3
-
 ?
3.1.1.107 diglucosyl-enterobactin + H2O Escherichia coli CFT073
-
 diglucosyl-(2,3-dihydroxybenzoylserine)3
-
 ?
3.1.1.107 enterobactin + H2O Escherichia coli
-
 N-(2,3-dihydroxybenzoyl)-L-serine trimer
-
 ?
3.1.1.107 enterobactin + H2O Escherichia coli CFT073
-
 N-(2,3-dihydroxybenzoyl)-L-serine trimer
-
 ?
3.1.1.107 monoglucosyl-enterobactin + H2O Escherichia coli
-
 monoglucosyl-(2,3-dihydroxybenzoylserine)3
-
 ?
3.1.1.107 monoglucosyl-enterobactin + H2O Escherichia coli CFT073
-
 monoglucosyl-(2,3-dihydroxybenzoylserine)3
-
 ?
3.1.1.107 triglucosyl-enterobactin + H2O Escherichia coli
-
 triglucosyl-(2,3-dihydroxybenzoylserine)3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.107 Escherichia coli A0A0H2V871
-
-
3.1.1.107 Escherichia coli CFT073 A0A0H2V871
-
-
3.1.1.108 Escherichia coli
-
-
-
3.1.1.108 Escherichia coli CFT073
-
-
-
3.1.1.109 Escherichia coli A0A0H2V660
-
-
3.1.1.109 Escherichia coli CFT073 A0A0H2V660
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.107 Ni-NTA column chromatography Escherichia coli
3.1.1.108 Ni-NTA column chromatography, Mono Q column chromatography, and Superdex S200 gel filtration Escherichia coli
3.1.1.109 Ni-NTA column chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.107 diglucosyl-enterobactin + H2O
-
 Escherichia coli diglucosyl-(2,3-dihydroxybenzoylserine)3
-
 ?
3.1.1.107 diglucosyl-enterobactin + H2O
-
 Escherichia coli CFT073 diglucosyl-(2,3-dihydroxybenzoylserine)3
-
 ?
3.1.1.107 enterobactin + H2O
-
 Escherichia coli N-(2,3-dihydroxybenzoyl)-L-serine trimer
-
 ?
3.1.1.107 enterobactin + H2O the enzyme prefers apo siderophores as substrates, and tends to hydrolyze the trilactone just once to produce linearized trimers Escherichia coli N-(2,3-dihydroxybenzoyl)-L-serine trimer
-
 ?
3.1.1.107 enterobactin + H2O
-
 Escherichia coli CFT073 N-(2,3-dihydroxybenzoyl)-L-serine trimer
-
 ?
3.1.1.107 enterobactin + H2O the enzyme prefers apo siderophores as substrates, and tends to hydrolyze the trilactone just once to produce linearized trimers Escherichia coli CFT073 N-(2,3-dihydroxybenzoyl)-L-serine trimer
-
 ?
3.1.1.107 ferric diglucosyl-enterobactin + H2O
-
 Escherichia coli ferric diglucosyl-(2,3-dihydroxybenzoylserine)3
-
 ?
3.1.1.107 ferric enterobactin + H2O
-
 Escherichia coli ferric N-(2,3-dihydroxybenzoyl)-L-serine trimer
-
 ?
3.1.1.107 ferric monoglucosyl-enterobactin + H2O
-
 Escherichia coli ferric monoglucosyl-(2,3-dihydroxybenzoylserine)3
-
 ?
3.1.1.107 ferric triglucosyl-enterobactin + H2O
-
 Escherichia coli triglucosyl-(2,3-dihydroxybenzoylserine)3
-
 ?
3.1.1.107 monoglucosyl-enterobactin + H2O
-
 Escherichia coli monoglucosyl-(2,3-dihydroxybenzoylserine)3
-
 ?
3.1.1.107 monoglucosyl-enterobactin + H2O
-
 Escherichia coli CFT073 monoglucosyl-(2,3-dihydroxybenzoylserine)3
-
 ?
3.1.1.107 additional information the enzyme hydrolyzes the Fe3+-bound siderophores very inefficiently. The enzyme hydrolyzes apo forms of siderophores more efficiently than Fe3+-bound ones Escherichia coli ?
-
 ?
3.1.1.107 additional information the enzyme hydrolyzes the Fe3+-bound siderophores very inefficiently. The enzyme hydrolyzes apo forms of siderophores more efficiently than Fe3+-bound ones Escherichia coli CFT073 ?
-
 ?
3.1.1.107 triglucosyl-enterobactin + H2O
-
 Escherichia coli triglucosyl-(2,3-dihydroxybenzoylserine)3
-
 ?
3.1.1.108 diglucosyl-enterobactin + H2O poor substrate Escherichia coli diglucosyl-(2,3-dihydroxybenzoylserine)3
-
 ?
3.1.1.108 diglucosyl-enterobactin + H2O poor substrate Escherichia coli CFT073 diglucosyl-(2,3-dihydroxybenzoylserine)3
-
 ?
3.1.1.108 enterobactin + H2O
-
 Escherichia coli N-(2,3-dihydroxybenzoyl)-L-serine trimer
-
 ?
3.1.1.108 enterobactin + H2O
-
 Escherichia coli CFT073 N-(2,3-dihydroxybenzoyl)-L-serine trimer
-
 ?
3.1.1.108 iron(III)-enterobactin + H2O highest catalytic efficiency Escherichia coli iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine trimer complex + N-(2,3-dihydroxybenzoyl)-L-serine
-
 ?
3.1.1.108 iron(III)-enterobactin + H2O highest catalytic efficiency Escherichia coli CFT073 iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine trimer complex + N-(2,3-dihydroxybenzoyl)-L-serine
-
 ?
3.1.1.108 iron(III)-monoglucosyl-enterobactin + H2O
-
 Escherichia coli ?
-
 ?
3.1.1.108 monoglucosyl-enterobactin + H2O
-
 Escherichia coli monoglucosyl-(2,3-dihydroxybenzoylserine)3
-
 ?
3.1.1.108 monoglucosyl-enterobactin + H2O
-
 Escherichia coli CFT073 monoglucosyl-(2,3-dihydroxybenzoylserine)3
-
 ?
3.1.1.108 additional information no activity with triglucosyl-enterobactin, iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine dimer complex and iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine trimer complex Escherichia coli ?
-
 ?
3.1.1.108 additional information no activity with triglucosyl-enterobactin, iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine dimer complex and iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine trimer complex Escherichia coli CFT073 ?
-
 ?
3.1.1.109 enterobactin + H2O
-
 Escherichia coli ?
-
 ?
3.1.1.109 enterobactin + H2O
-
 Escherichia coli CFT073 ?
-
 ?
3.1.1.109 iron(III)-enterobactin + H2O
-
 Escherichia coli ?
-
 ?
3.1.1.109 iron(III)-enterobactin + H2O
-
 Escherichia coli CFT073 ?
-
 ?
3.1.1.109 iron(III)-[diglucosyl-enterobactin] complex + H2O the enzyme shows a kcat/Km preference for the Fe3+-bound form over the apo form of the substrate Escherichia coli iron(III)-[salmochelin S2] complex
-
 ?
3.1.1.109 iron(III)-[diglucosyl-enterobactin] complex + H2O the enzyme shows a kcat/Km preference for the Fe3+-bound form over the apo form of the substrate Escherichia coli CFT073 iron(III)-[salmochelin S2] complex
-
 ?
3.1.1.109 iron(III)-[monoglucosyl-enterobactin] complex + H2O the enzyme shows a kcat/Km preference for the Fe3+-bound form over the apo form of the substrate Escherichia coli iron(III)-[monoglucosyl-(2,3-dihydroxybenzoylserine)3] complex
-
 ?
3.1.1.109 iron(III)-[monoglucosyl-enterobactin] complex + H2O the enzyme shows a kcat/Km preference for the Fe3+-bound form over the apo form of the substrate Escherichia coli CFT073 iron(III)-[monoglucosyl-(2,3-dihydroxybenzoylserine)3] complex
-
 ?
3.1.1.109 iron(III)-[triglucosyl-enterobactin] complex + H2O the enzyme shows a kcat/Km preference for the Fe3+-bound form over the apo form of the substrate Escherichia coli ?
-
 ?
3.1.1.109 additional information the enzyme shows a kcat/Km preference for the Fe3+-bound form of over the apo form of the substrate Escherichia coli ?
-
 ?
3.1.1.109 additional information the enzyme shows a kcat/Km preference for the Fe3+-bound form of over the apo form of the substrate Escherichia coli CFT073 ?
-
 ?
3.1.1.109 [diglucosyl-enterobactin] complex + H2O
-
 Escherichia coli [salmochelin S2] complex
-
 ?
3.1.1.109 [monoglucosyl-enterobactin] complex + H2O
-
 Escherichia coli [monoglucosyl-(2,3-dihydroxybenzoylserine)3] complex
-
 ?
3.1.1.109 [triglucosyl-enterobactin] complex + H2O
-
 Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.107 ? x * 35000, SDS-PAGE Escherichia coli
3.1.1.108 ? x * 45000, SDS-PAGE Escherichia coli
3.1.1.108 ? x * 43000, calculated from amino acid sequence Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
3.1.1.107 iroE
-
 Escherichia coli
3.1.1.108 ferric Ent esterase
-
 Escherichia coli
3.1.1.108 Fes
-
 Escherichia coli
3.1.1.109 Fe-MGE/Fe-DGE esterase
-
 Escherichia coli
3.1.1.109 ferric MGE/DGE esterase
-
 Escherichia coli
3.1.1.109 IroD
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.107 0.04
-
 ferric diglucosyl-enterobactin at pH 7.5 and 23°C Escherichia coli
3.1.1.107 0.05
-
 ferric enterobactin at pH 7.5 and 23°C Escherichia coli
3.1.1.107 0.05
-
 ferric monoglucosyl-enterobactin at pH 7.5 and 23°C Escherichia coli
3.1.1.107 0.13
-
 ferric triglucosyl-enterobactin turnover number above 0.13 1/sec, at pH 7.5 and 23°C Escherichia coli
3.1.1.107 5.33
-
 diglucosyl-enterobactin at pH 7.5 and 23°C Escherichia coli
3.1.1.107 6.25
-
 enterobactin at pH 7.5 and 23°C Escherichia coli
3.1.1.107 7.17
-
 monoglucosyl-enterobactin at pH 7.5 and 23°C Escherichia coli
3.1.1.107 7.5
-
 triglucosyl-enterobactin at pH 7.5 and 23°C Escherichia coli
3.1.1.108 0.023
-
 iron(III)-monoglucosyl-enterobactin at pH 7.5 and 23°C Escherichia coli
3.1.1.108 0.15
-
 iron(III)-enterobactin at pH 7.5 and 23°C Escherichia coli
3.1.1.108 1.3
-
 diglucosyl-enterobactin at pH 7.5 and 23°C Escherichia coli
3.1.1.108 8.67
-
 enterobactin at pH 7.5 and 23°C Escherichia coli
3.1.1.108 14.17
-
 monoglucosyl-enterobactin at pH 7.5 and 23°C Escherichia coli
3.1.1.109 0.53
-
 iron(III)-[diglucosyl-enterobactin] complex at pH 7.5 and 23°C Escherichia coli
3.1.1.109 0.77
-
 iron(III)-[monoglucosyl-enterobactin] complex at pH 7.5 and 23°C Escherichia coli
3.1.1.109 1.23
-
 iron(III)-enterobactin at pH 7.5 and 23°C Escherichia coli
3.1.1.109 1.4
-
 iron(III)-[triglucosyl-enterobactin] complex at pH 7.5 and 23°C Escherichia coli
3.1.1.109 17.67
-
 enterobactin at pH 7.5 and 23°C Escherichia coli
3.1.1.109 62
-
 [monoglucosyl-enterobactin] complex at pH 7.5 and 23°C Escherichia coli
3.1.1.109 74.33
-
 [triglucosyl-enterobactin] complex at pH 7.5 and 23°C Escherichia coli
3.1.1.109 108.33
-
 [diglucosyl-enterobactin] complex at pH 7.5 and 23°C Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.107 8.3
-
 ferric diglucosyl-enterobactin at pH 7.5 and 23°C Escherichia coli
3.1.1.107 8.3
-
 ferric triglucosyl-enterobactin at pH 7.5 and 23°C Escherichia coli
3.1.1.107 11.7
-
 ferric monoglucosyl-enterobactin at pH 7.5 and 23°C Escherichia coli
3.1.1.107 15
-
 ferric enterobactin at pH 7.5 and 23°C Escherichia coli
3.1.1.107 50
-
 triglucosyl-enterobactin at pH 7.5 and 23°C Escherichia coli
3.1.1.107 133
-
 diglucosyl-enterobactin at pH 7.5 and 23°C Escherichia coli
3.1.1.107 233
-
 monoglucosyl-enterobactin at pH 7.5 and 23°C Escherichia coli
3.1.1.107 383
-
 enterobactin at pH 7.5 and 23°C Escherichia coli
3.1.1.108 10
-
 diglucosyl-enterobactin at pH 7.5 and 23°C Escherichia coli
3.1.1.108 66.7
-
 enterobactin at pH 7.5 and 23°C Escherichia coli
3.1.1.108 80
-
 iron(III)-monoglucosyl-enterobactin at pH 7.5 and 23°C Escherichia coli
3.1.1.108 141.7
-
 monoglucosyl-enterobactin at pH 7.5 and 23°C Escherichia coli
3.1.1.108 1500
-
 iron(III)-enterobactin at pH 7.5 and 23°C Escherichia coli
3.1.1.109 433
-
 enterobactin at pH 7.5 and 23°C Escherichia coli
3.1.1.109 467
-
 [triglucosyl-enterobactin] complex at pH 7.5 and 23°C Escherichia coli
3.1.1.109 900
-
 [diglucosyl-enterobactin] complex at pH 7.5 and 23°C Escherichia coli
3.1.1.109 1033
-
 [monoglucosyl-enterobactin] complex at pH 7.5 and 23°C Escherichia coli
3.1.1.109 3250
-
 iron(III)-[triglucosyl-enterobactin] complex at pH 7.5 and 23°C Escherichia coli
3.1.1.109 4450
-
 iron(III)-[diglucosyl-enterobactin] complex at pH 7.5 and 23°C Escherichia coli
3.1.1.109 9583
-
 iron(III)-[monoglucosyl-enterobactin] complex at pH 7.5 and 23°C Escherichia coli
3.1.1.109 10283
-
 iron(III)-enterobactin at pH 7.5 and 23°C Escherichia coli