EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.4.21.63 | acetone | activates 2fold at 30% v/v | Aspergillus oryzae | |
3.4.21.63 | hexane | activates slightly at 15% v/v | Aspergillus oryzae | |
3.4.21.63 | isoamyl alcohol | activates 1.5fold at 30% v/v | Aspergillus oryzae |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.21.63 | 2-mercaptoethanol | - |
Aspergillus oryzae | |
3.4.21.63 | 2-propanol | inhibits at 30% v/v | Aspergillus oryzae | |
3.4.21.63 | cetyltrimethyl ammonium bromide | - |
Aspergillus oryzae | |
3.4.21.63 | DMSO | inhibits at 30% v/v | Aspergillus oryzae | |
3.4.21.63 | EDTA | - |
Aspergillus oryzae | |
3.4.21.63 | H2O2 | - |
Aspergillus oryzae | |
3.4.21.63 | Isopropanol | inhibits at 30% v/v | Aspergillus oryzae | |
3.4.21.63 | additional information | Triton X-100 and PMSF have no inhibitory effect on the enzyme activity | Aspergillus oryzae | |
3.4.21.63 | SDS | - |
Aspergillus oryzae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.21.63 | additional information | - |
additional information | casein gives a Vmax of 141.1 ng/min and a Km of 2432 ng/ml | Aspergillus oryzae |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.4.21.63 | extracellular | - |
Aspergillus oryzae | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.21.63 | Ca2+ | 19% activation at 5 mM, no activation at 2 mM | Aspergillus oryzae | |
3.4.21.63 | Fe2+ | 13% activation at 2 mM, 40% at 5 mM | Aspergillus oryzae | |
3.4.21.63 | Mg2+ | 20% activation at 5 mM, no activation at 2 mM | Aspergillus oryzae | |
3.4.21.63 | Mn2+ | 21% activation at 2 mM, 14% at 5 mM | Aspergillus oryzae | |
3.4.21.63 | additional information | no significant effect with Mn2+ and Cu2+ at 2-5 mM | Aspergillus oryzae | |
3.4.21.63 | Na+ | 14% activation at 2 mM, no activation at 5 mM | Aspergillus oryzae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.21.63 | Aspergillus oryzae | - |
isolated from soil sample from Charm-Shahr of Mashhad located in the countryside of Mashhad-Iran | - |
3.4.21.63 | Aspergillus oryzae CH93 | - |
isolated from soil sample from Charm-Shahr of Mashhad located in the countryside of Mashhad-Iran | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.21.63 | native enzyme 1.32fold by ammonium sulfate fractionation, and anion exchange chromatography | Aspergillus oryzae |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.4.21.63 | mycelium | cultivation on solid medium, optimization of culture conditions, overview | Aspergillus oryzae | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.21.63 | azocasein + H2O | - |
Aspergillus oryzae | ? | - |
? | |
3.4.21.63 | azocasein + H2O | - |
Aspergillus oryzae CH93 | ? | - |
? | |
3.4.21.63 | Bovine serum albumin + H2O | - |
Aspergillus oryzae | ? | - |
? | |
3.4.21.63 | Bovine serum albumin + H2O | - |
Aspergillus oryzae CH93 | ? | - |
? | |
3.4.21.63 | casein + H2O | best substrate | Aspergillus oryzae | ? | - |
? | |
3.4.21.63 | casein + H2O | best substrate | Aspergillus oryzae CH93 | ? | - |
? | |
3.4.21.63 | Gelatin + H2O | low activity | Aspergillus oryzae | ? | - |
? | |
3.4.21.63 | Gelatin + H2O | low activity | Aspergillus oryzae CH93 | ? | - |
? | |
3.4.21.63 | N-acetyl-L-tyrosine ethylester + H2O | low activity | Aspergillus oryzae | ? | - |
? | |
3.4.21.63 | N-acetyl-L-tyrosine ethylester + H2O | low activity | Aspergillus oryzae CH93 | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.21.63 | Alkaline protease | - |
Aspergillus oryzae |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.21.63 | 50 | - |
- |
Aspergillus oryzae |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.21.63 | 50 | - |
purified enzyme, half-life is 100 min | Aspergillus oryzae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.21.63 | 8 | - |
- |
Aspergillus oryzae |