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Literature summary extracted from

  • Salihi, A.; Asoodeh, A.; Aliabadian, M.
    Production and biochemical characterization of an alkaline protease from Aspergillus oryzae CH93 (2017), Int. J. Biol. Macromol., 94, 827-835 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.21.63 acetone activates 2fold at 30% v/v Aspergillus oryzae
3.4.21.63 hexane activates slightly at 15% v/v Aspergillus oryzae
3.4.21.63 isoamyl alcohol activates 1.5fold at 30% v/v Aspergillus oryzae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.63 2-mercaptoethanol
-
Aspergillus oryzae
3.4.21.63 2-propanol inhibits at 30% v/v Aspergillus oryzae
3.4.21.63 cetyltrimethyl ammonium bromide
-
Aspergillus oryzae
3.4.21.63 DMSO inhibits at 30% v/v Aspergillus oryzae
3.4.21.63 EDTA
-
Aspergillus oryzae
3.4.21.63 H2O2
-
Aspergillus oryzae
3.4.21.63 Isopropanol inhibits at 30% v/v Aspergillus oryzae
3.4.21.63 additional information Triton X-100 and PMSF have no inhibitory effect on the enzyme activity Aspergillus oryzae
3.4.21.63 SDS
-
Aspergillus oryzae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.63 additional information
-
additional information casein gives a Vmax of 141.1 ng/min and a Km of 2432 ng/ml Aspergillus oryzae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.21.63 extracellular
-
Aspergillus oryzae
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.21.63 Ca2+ 19% activation at 5 mM, no activation at 2 mM Aspergillus oryzae
3.4.21.63 Fe2+ 13% activation at 2 mM, 40% at 5 mM Aspergillus oryzae
3.4.21.63 Mg2+ 20% activation at 5 mM, no activation at 2 mM Aspergillus oryzae
3.4.21.63 Mn2+ 21% activation at 2 mM, 14% at 5 mM Aspergillus oryzae
3.4.21.63 additional information no significant effect with Mn2+ and Cu2+ at 2-5 mM Aspergillus oryzae
3.4.21.63 Na+ 14% activation at 2 mM, no activation at 5 mM Aspergillus oryzae

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.63 Aspergillus oryzae
-
isolated from soil sample from Charm-Shahr of Mashhad located in the countryside of Mashhad-Iran
-
3.4.21.63 Aspergillus oryzae CH93
-
isolated from soil sample from Charm-Shahr of Mashhad located in the countryside of Mashhad-Iran
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.63 native enzyme 1.32fold by ammonium sulfate fractionation, and anion exchange chromatography Aspergillus oryzae

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.63 mycelium cultivation on solid medium, optimization of culture conditions, overview Aspergillus oryzae
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.63 azocasein + H2O
-
Aspergillus oryzae ?
-
?
3.4.21.63 azocasein + H2O
-
Aspergillus oryzae CH93 ?
-
?
3.4.21.63 Bovine serum albumin + H2O
-
Aspergillus oryzae ?
-
?
3.4.21.63 Bovine serum albumin + H2O
-
Aspergillus oryzae CH93 ?
-
?
3.4.21.63 casein + H2O best substrate Aspergillus oryzae ?
-
?
3.4.21.63 casein + H2O best substrate Aspergillus oryzae CH93 ?
-
?
3.4.21.63 Gelatin + H2O low activity Aspergillus oryzae ?
-
?
3.4.21.63 Gelatin + H2O low activity Aspergillus oryzae CH93 ?
-
?
3.4.21.63 N-acetyl-L-tyrosine ethylester + H2O low activity Aspergillus oryzae ?
-
?
3.4.21.63 N-acetyl-L-tyrosine ethylester + H2O low activity Aspergillus oryzae CH93 ?
-
?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.63 Alkaline protease
-
Aspergillus oryzae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.63 50
-
-
Aspergillus oryzae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.63 50
-
purified enzyme, half-life is 100 min Aspergillus oryzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.63 8
-
-
Aspergillus oryzae