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Literature summary extracted from

  • Abdel Wahab, W.A.; Ahmed, S.A.
    Response surface methodology for production, characterization and application of solvent, salt and alkali-tolerant alkaline protease from isolated fungal strain Aspergillus niger WA 2017 (2018), Int. J. Biol. Macromol., 115, 447-458 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.21.63 Triton X-100 activates 20% at 0.5% Aspergillus niger
3.4.21.63 Tween 80 slight activation at 0.5% Aspergillus niger

Application

EC Number Application Comment Organism
3.4.21.63 additional information the produced enzyme is able to recover silver from used X-ray film under optimized condition, overview Aspergillus niger

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.63 butanol 50% inhibition after 2 h Aspergillus niger
3.4.21.63 EDTA
-
 Aspergillus niger
3.4.21.63 H2O2
-
 Aspergillus niger
3.4.21.63 iodoacetic acid strong inhibition at 5 mM Aspergillus niger
3.4.21.63 additional information the enzyme is completely stable in presence of laundry detergents, overview Aspergillus niger
3.4.21.63 PMSF strong inhibition at 5 mM Aspergillus niger
3.4.21.63 SDS strong inhibition at 0.5% Aspergillus niger
3.4.21.63 Urea slight inhibition at 5 mM Aspergillus niger

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.21.63 NaCl activates at up to 12% w/v, the enzyme is stable up to 20% w/v, thermal stability of the enzyme is substantially improved by NaCl Aspergillus niger

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.4.21.63 Butanol 50% inhibition after 2 h Aspergillus niger
3.4.21.63 Ethanol the enzyme shows excellent stability and compatibility in presence of organic solvents and detergents retaining 115.3% of its activity in presence of ethanol at 40°C for 1 h Aspergillus niger
3.4.21.63 additional information the enzyme is highly stable in different organic solvents at 40°C for 1-2 h, e.g. in ethanol, methanol, butanol, acetone, benzene, ethyl acetate, and chloroform, all at the concentration of 25% v/v Aspergillus niger
3.4.21.63 additional information the enzyme shows excellent stability and compatibility in presence of commercial detergent Tide retaining 114.5% of its activity in presence of 1% (w/v) at 40°C for 1 h Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.63 Aspergillus niger
-
-
-
3.4.21.63 Aspergillus niger WA 2017
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.63 additional information the enzyme production is increased by 3.6fold with statistically optimized medium when compared to the basal medium, optimization, overview Aspergillus niger
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.63 casein + H2O
-
 Aspergillus niger ?
-
 ?
3.4.21.63 casein + H2O
-
 Aspergillus niger WA 2017 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.63 Alkaline protease
-
 Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.63 60
-
-
 Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.63 additional information
-
 the thermal stability of the enzyme is substantially improved by NaCl Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.63 10
-
-
 Aspergillus niger

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.21.63 7 11 stable at Aspergillus niger