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Literature summary extracted from

  • Nakagawa, T.; Shimada, Y.; Pavlova, N.V.; Li, S.C.; Li, Y.T.
    Cloning and expression of 3-deoxy-D-manno-oct-2-ulosonic acid alpha-ketoside hydrolase from oyster hepatopancreas (2015), Glycobiology, 25, 1431-1440 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.144 DNA and amino acid sequence determination and analysis, sequence comparisons, functional recombinant expression in Saccharomyces cerevisiae and CHO-S cells, expression in Escherichia coli strains is not successful Crassostrea virginica

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.144 extracellular
-
 Crassostrea virginica
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.144 additional information Crassostrea virginica the recombinant alpha-Kdo-ase effectively hydrolyzes the alpha-ketoside in the core-oligosaccharide of the structurally defined lipopolysaccharide (LPS), Re-LPS (Kdo2-lipid A) from Salmonella minnesota strain R595 and Escherichia coli strain D31m4, while Rd-LPS from Salmonella minnesota strain R7, that contains an extra outer core phosphorylated heptose, is only slowly hydrolyzed. The complex type LPS from Neisseria meningitides strains A1 and M992 that contain extra 5-6 sugar units at the outer core are refractory to recombinnat alpha-Kdo-ase ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.144 Crassostrea virginica A0A0M3N009
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.144 recombinant extracellular enzyme from Pichia pastoris and Saccharomyces cerevisiae in large scale by ultracentrifugation of culture supernatant, followed by two steps ultrafiltration and gel filtration, recombinnat His-tagged enzyme from Pichia pastoris by nickel affinity chromatography and gel filtration Crassostrea virginica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.144 hepatopancreas
-
 Crassostrea virginica
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.144 4-methylumbelliferyl 3-deoxy-alpha-D-manno-oct-2-ulosonic acid + H2O
-
 Crassostrea virginica 4-methylumbelliferone + 3-deoxy-D-manno-oct-2-ulosonic acid
-
 ?
3.2.1.144 additional information the recombinant alpha-Kdo-ase effectively hydrolyzes the alpha-ketoside in the core-oligosaccharide of the structurally defined lipopolysaccharide (LPS), Re-LPS (Kdo2-lipid A) from Salmonella minnesota strain R595 and Escherichia coli strain D31m4, while Rd-LPS from Salmonella minnesota strain R7, that contains an extra outer core phosphorylated heptose, is only slowly hydrolyzed. The complex type LPS from Neisseria meningitides strains A1 and M992 that contain extra 5-6 sugar units at the outer core are refractory to recombinnat alpha-Kdo-ase Crassostrea virginica ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.144 ? x * 44604, sequence calculation Crassostrea virginica

Synonyms

EC Number Synonyms Comment Organism
3.2.1.144 3-deoxy-D-manno-oct-2-ulosonic acid alpha-ketoside hydrolase
-
 Crassostrea virginica
3.2.1.144 3-deoxy-octulo-sonase
-
 Crassostrea virginica
3.2.1.144 alpha-Kdo-ase
-
 Crassostrea virginica

General Information

EC Number General Information Comment Organism
3.2.1.144 evolution the deduced amino acid sequence of the enzyme contains two Asp boxes (S277PDDGKTW and S328TDQGKTW) commonly found in sialidases Crassostrea virginica