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Literature summary extracted from
- The outer membrane protease PgtE of Salmonella enterica interferes with the alternative complement pathway by cleaving factors B and H (2015), Front. Microbiol., 6, 63 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.23.49 | recombinant expression of wild-type pgtE in Escherichia coli strain 83972 DELTAompT, which is deleted for the endogenous omptin gene ompT and lacks ompP. Escherichia coli strain 83972 DELTAompT expressing PgtE cleaves B in a time-dependent fashion generating four immunoreactive fragments | Salmonella enterica subsp. enterica serovar Typhimurium |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.4.23.49 | D206A | site-directed mutagenesis, inacticve catalytic site mutant | Salmonella enterica subsp. enterica serovar Typhimurium |
| 3.4.23.49 | additional information | construction of Salmonella enterica mutant DELTApgtE strain 14028R-1 from the isogenic virulent wild-type strain 14028R. Mutant strain 14028R-1 is inactive with factor H and B. Complementation of 14028R-1 with pgtE-encoding pMRK3 recovers the activity toward B and H | Salmonella enterica subsp. enterica serovar Typhimurium |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.23.49 | factor B + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | - |
? | - |
? |  |
| 3.4.23.49 | factor B + H2O | Salmonella enterica subsp. enterica serovar Typhimurium 14028R | - |
? | - |
? | |
| 3.4.23.49 | factor H + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | factor H is cleaved at both N- and C-termini, while the central region resists proteolysis | ? | - |
? | |
| 3.4.23.49 | factor H + H2O | Salmonella enterica subsp. enterica serovar Typhimurium 14028R | factor H is cleaved at both N- and C-termini, while the central region resists proteolysis | ? | - |
? | |
| 3.4.23.49 | additional information | Salmonella enterica subsp. enterica serovar Typhimurium | PgtE cleaves both B and H, whereas its close homologue Pla of Yersinia pestis (EC 3.4.23.48) cleaves only H | ? | - |
? | |
| 3.4.23.49 | additional information | Salmonella enterica subsp. enterica serovar Typhimurium 14028R | PgtE cleaves both B and H, whereas its close homologue Pla of Yersinia pestis (EC 3.4.23.48) cleaves only H | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.23.49 | Salmonella enterica subsp. enterica serovar Typhimurium | - |
the rough variant 14028R is resistant to a smooth LPS-specific bacteriophage P22c2 | - |
| 3.4.23.49 | Salmonella enterica subsp. enterica serovar Typhimurium 14028R | - |
the rough variant 14028R is resistant to a smooth LPS-specific bacteriophage P22c2 | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.23.49 | factor B + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
| 3.4.23.49 | factor B + H2O | PgtE generates B fragments of approximately 57 kDa and 28 kDa under non-reducing conditions, while under reducing conditions, the cleavage pattern is different lacking the 28 kDa fragment. PgtE cleaves H near both termini. Mapping of cleavage sites in factor H, overview | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
| 3.4.23.49 | factor B + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium 14028R | ? | - |
? | |
| 3.4.23.49 | factor B + H2O | PgtE generates B fragments of approximately 57 kDa and 28 kDa under non-reducing conditions, while under reducing conditions, the cleavage pattern is different lacking the 28 kDa fragment. PgtE cleaves H near both termini. Mapping of cleavage sites in factor H, overview | Salmonella enterica subsp. enterica serovar Typhimurium 14028R | ? | - |
? | |
| 3.4.23.49 | factor H + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
| 3.4.23.49 | factor H + H2O | factor H is cleaved at both N- and C-termini, while the central region resists proteolysis | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
| 3.4.23.49 | factor H + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium 14028R | ? | - |
? | |
| 3.4.23.49 | factor H + H2O | factor H is cleaved at both N- and C-termini, while the central region resists proteolysis | Salmonella enterica subsp. enterica serovar Typhimurium 14028R | ? | - |
? | |
| 3.4.23.49 | additional information | PgtE cleaves both B and H, whereas its close homologue Pla of Yersinia pestis (EC 3.4.23.48) cleaves only H | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
| 3.4.23.49 | additional information | PgtE cleaves both B and H, whereas its close homologue Pla of Yersinia pestis (EC 3.4.23.48) cleaves only H | Salmonella enterica subsp. enterica serovar Typhimurium 14028R | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.23.49 | outer membrane protease | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
| 3.4.23.49 | PgtE | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.4.23.49 | malfunction | human neutrophils interact less with serum-opsonized FITC-stained Salmonella enterica strain 14028R than with the isogenic DELTApgtE strain 14028R-1 | Salmonella enterica subsp. enterica serovar Typhimurium |
| 3.4.23.49 | metabolism | the breakdown of factors B and H is critically dependent on the direct proteolytic activity exerted by omptins | Salmonella enterica subsp. enterica serovar Typhimurium |
| 3.4.23.49 | physiological function | the virulence factor PgtE is an outer membrane protease (omptin) of the zoonotic pathogen Salmonella enterica. PgtE of Salmonella enterica interferes with the alternative complement pathway by cleaving factors B and H. In human serum, C3 cleavage is dependent on proteolytically active PgtE. PgtE inhibits opsonization of Slamonella enterica strain 14028R. Cleavage of H abolishes its complement regulatory activity leading to increased formation of C3b, whereas cleavage of B leads to fewer active C3 convertases and decreased formation of C3b. PgtE competes with B and H for C3/C3b cleavage, because B fragment Bb in the C3-convertase cleaves C3, and H is a cofactor for C3b cleavage by factor I | Salmonella enterica subsp. enterica serovar Typhimurium |
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