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Literature summary extracted from

  • Chambon, R.; Pradeau, S.; Fort, S.; Cottaz, S.; Armand, S.
    High yield production of Rhizobium NodB chitin deacetylase and its use for in vitro synthesis of lipo-chitinoligosaccharide precursors (2017), Carbohydr. Res., 442, 25-30 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.41 gene nodB, recombinant overexpression of His-tagged enzyme NodB in Escherichia coli strain BL21(DE3), recombinant expression of Trx-NodB. The recombinant enzyme expressed in a soluble and catalytically active form is then efficiently used as biocatalyst Sinorhizobium meliloti

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.41 0.379
-
 GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcN-beta-(1-4)-GlcNAc pH 7.2, 30°C, recombinant His-tagged enzyme Sinorhizobium meliloti
3.5.1.41 1.53
-
 GlcNAc-beta-(1-4)-GlcNAc pH 7.2, 30°C, recombinant His-tagged enzyme Sinorhizobium meliloti

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.41 Sinorhizobium meliloti P02963 i.e. Ensifer meliloti or Sinorhizobium meliloti
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.41 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Sinorhizobium meliloti

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.41 acetylated chitosan polymer + H2O increasing activity with increasing chain length Sinorhizobium meliloti ?
-
 ?
3.5.1.41 GlcNAc-beta-(1-4)-GlcNAc + H2O
-
 Sinorhizobium meliloti GlcN-beta-(1-4)-GlcN + 2 acetate
-
 ?
3.5.1.41 GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcN-beta-(1-4)-GlcNAc + H2O
-
 Sinorhizobium meliloti ?
-
 ?
3.5.1.41 additional information the reaction mechanism governs the regioselectivity Sinorhizobium meliloti ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.41 ? x * 28500, recombinant His-tagged enzyme, SDS-PAGE Sinorhizobium meliloti

Synonyms

EC Number Synonyms Comment Organism
3.5.1.41 NodB
-
 Sinorhizobium meliloti

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.41 30
-
 recombinant enzyme Sinorhizobium meliloti

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.41 0.38
-
 GlcNAc-beta-(1-4)-GlcNAc pH 7.2, 30°C, recombinant His-tagged enzyme Sinorhizobium meliloti
3.5.1.41 0.45
-
 GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcN-beta-(1-4)-GlcNAc pH 7.2, 30°C, recombinant His-tagged enzyme Sinorhizobium meliloti

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.41 7 8 recombinant enzyme Sinorhizobium meliloti

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.1.41 0.248
-
 GlcNAc-beta-(1-4)-GlcNAc pH 7.2, 30°C, recombinant His-tagged enzyme Sinorhizobium meliloti
3.5.1.41 1.19
-
 GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcN-beta-(1-4)-GlcNAc pH 7.2, 30°C, recombinant His-tagged enzyme Sinorhizobium meliloti