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Literature summary extracted from

  • Kong, J.; Li, Z.; Zhang, H.; Gao, X.D.; Nakanishi, H.
    Production of encapsulated creatinase using yeast spores (2017), Bioengineered, 8, 411-419 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.5.3.3 diagnostics yeast spore-based encapsulation technique is applied to the diagnostic enzyme creatinase. A secretory form of Pseudomonas putida creatinase can be entrapped in the spore wall, and such spores are used as creatinase capsules. The activity of the encapsulated creatinase is largely improved by mild spore wall defective mutations, such as DIT1 or OSW2 deletions. The encapsulated and freeze-dried creatinase is produced without preparing the purified enzyme, and it exhibits resistance to environmental stresses, such as high temperature and SDS treatments. Thus, yeast spores can be applied to establish quick and easy clinical diagnostic methods Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.3.3 7
-
 Creatine pH 7.7, 50°C, creatinase in yeast spores with DIT1 deletion Pseudomonas putida
3.5.3.3 7
-
 Creatine pH 7.7, 50°C, soluble enzyme Pseudomonas putida
3.5.3.3 7.8
-
 Creatine pH 7.7, 50°C, creatinase in yeast spores with OSW2 deletion Pseudomonas putida
3.5.3.3 10.9
-
 Creatine pH 7.7, 50°C, creatinase in wild-type yeast spores Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.3.3 creatine + H2O Pseudomonas putida
-
 sarcosine + urea
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.3 Pseudomonas putida P38488
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.3 creatine + H2O
-
 Pseudomonas putida sarcosine + urea
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.3.3 80 90 the activities of creatinase in wild-type and osw2D spores are more stable than those of free creatinase at higher temperatures. Such thermostability is not observed for creatinase in dit1D spores Pseudomonas putida