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Literature summary extracted from

  • Ruggiero, A.; Garcia-Ortega, L.; Ragucci, S.; Russo, R.; Landi, N.; Berisio, R.; Di Maro, A.
    Structural and enzymatic properties of Ageritin, a novel metal-dependent ribotoxin-like protein with antitumor activity (2018), Biochim. Biophys. Acta, 1862, 2888-2894 .
    View publication on PubMed

General Stability

EC Number General Stability Organism
4.6.1.23 in guanidinium hydrochloride, half-completion of denaturing transition occurs at 5.1 M Cyclocybe aegerita

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.6.1.23 EDTA 0.6 mM, complete loss of activity Cyclocybe aegerita

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.6.1.23 Mg2+ ageritin strongly binds magnesium ions with low micromolar affinity, KD value 308 nM Cyclocybe aegerita
4.6.1.23 additional information activity is strongly metal-dependent and completely suppressed in the presence of EDTA Cyclocybe aegerita

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.6.1.23 14400
-
 gel filtration Cyclocybe aegerita

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.23 Cyclocybe aegerita C0HLG3
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.6.1.23 additional information ageritin shows specific ribonucleolytic activity against the ribosomal sarcin-ricin loop in a rabbit reticulocytes assay Cyclocybe aegerita ?
-
 ?
4.6.1.23 rabbit reticulocyte 28S rRNA containing guanosine-adenosine pair + H2O
-
 Cyclocybe aegerita an [RNA fragment]-3'-adenosine-3'-phosphate + a 5'-a hydroxy-guanosine-3'-[RNA fragment]
-
 ?

Subunits

EC Number Subunits Comment Organism
4.6.1.23 monomer 1 * 16000, SDS-PAGE, 1 * 14803, calculated from sequence Cyclocybe aegerita

Synonyms

EC Number Synonyms Comment Organism
4.6.1.23 AAE3_01767
-
 Cyclocybe aegerita

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.6.1.23 78
-
 melting temperature Cyclocybe aegerita