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Literature summary extracted from

  • Landi, N.; Pacifico, S.; Ragucci, S.; Iglesias, R.; Piccolella, S.; Amici, A.; Di Giuseppe, A.M.A.; Di Maro, A.
    Purification, characterization and cytotoxicity assessment of ageritin The first ribotoxin from the basidiomycete mushroom Agrocybe aegerita (2017), Biochim. Biophys. Acta, 1861, 1113-1121 .
    View publication on PubMed

Application

EC Number Application Comment Organism
4.6.1.23 medicine enzyme displays cytotoxity against tumour cells. The ribotoxin dose, able to inhibit by 50% the mitochondrial redox activity of C6 glial cells, is equal to 4.58 microg/ml (0.316 microM). A ribotoxin dose of 8.41 microg/ml inhibits by 50% human SK-N-BE(2)-C neuroblastoma cells and of 9.46 microg/ml U-251 glioma cells Cyclocybe aegerita

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.6.1.23 17000
-
 gel filtration Cyclocybe aegerita

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.23 Cyclocybe aegerita C0HLG3
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.6.1.23 Saccharomyces cerevisiae rRNA containing guanosine-adenosine pair + H2O
-
 Cyclocybe aegerita an [RNA fragment]-3'-adenosine-3'-phosphate + a 5'-a hydroxy-guanosine-3'-[RNA fragment]
-
 ?

Subunits

EC Number Subunits Comment Organism
4.6.1.23 monomer 1 * 17000, SDS-PAGE Cyclocybe aegerita

Synonyms

EC Number Synonyms Comment Organism
4.6.1.23 AAE3_01767
-
 Cyclocybe aegerita
4.6.1.23 ageritin
-
 Cyclocybe aegerita

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.6.1.23 Cyclocybe aegerita isoelectric focusing
-
 9.5

General Information

EC Number General Information Comment Organism
4.6.1.23 physiological function purified ageritin is able to inhibit protein synthesis in a rabbit reticulocyte lysate, with an IC50 value of 133 pM Cyclocybe aegerita