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Literature summary extracted from

  • Shek, R.; Dattmore, D.A.; Stives, D.P.; Jackson, A.L.; Chatfield, C.H.; Hicks, K.A.; French, J.B.
    Structural and functional basis for targeting Campylobacter jejuni agmatine deiminase to overcome antibiotic resistance (2017), Biochemistry, 56, 6734-6742 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.5.3.12 drug development Campylobacter jejuni agmatine deiminase is a potentially important target for combatting antibiotic resistance Campylobacter jejuni subsp. jejuni serotype O:2

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.3.12 expression of the wild-type enzyme and the C315S mutant enzyme in Escherichia coli BL21 (DE3) Campylobacter jejuni subsp. jejuni serotype O:2

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.3.12 crystal structures of both the unliganded wild-type enzyme and a catalytic mutant of agmatine deiminase with the substrate, agamatine, bound Campylobacter jejuni subsp. jejuni serotype O:2

Protein Variants

EC Number Protein Variants Comment Organism
3.5.3.12 C315S enzymatically inactive form Campylobacter jejuni subsp. jejuni serotype O:2

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.3.12 0.07
-
 agmatine pH 7.0, 37°C Campylobacter jejuni subsp. jejuni serotype O:2

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.3.12 agmatine + H2O Campylobacter jejuni subsp. jejuni serotype O:2 the enzyme is involved in the biosynthesis of polyamines N-carbamoylputrescine + NH3
-
 ?
3.5.3.12 agmatine + H2O Campylobacter jejuni subsp. jejuni serotype O:2 ATCC 700819 the enzyme is involved in the biosynthesis of polyamines N-carbamoylputrescine + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.12 Campylobacter jejuni subsp. jejuni serotype O:2 Q0P9V0
-
-
3.5.3.12 Campylobacter jejuni subsp. jejuni serotype O:2 ATCC 700819 Q0P9V0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.3.12
-
 Campylobacter jejuni subsp. jejuni serotype O:2

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.12 agmatine + H2O
-
 Campylobacter jejuni subsp. jejuni serotype O:2 N-carbamoylputrescine + NH3
-
 ?
3.5.3.12 agmatine + H2O the enzyme is involved in the biosynthesis of polyamines Campylobacter jejuni subsp. jejuni serotype O:2 N-carbamoylputrescine + NH3
-
 ?
3.5.3.12 agmatine + H2O
-
 Campylobacter jejuni subsp. jejuni serotype O:2 ATCC 700819 N-carbamoylputrescine + NH3
-
 ?
3.5.3.12 agmatine + H2O the enzyme is involved in the biosynthesis of polyamines Campylobacter jejuni subsp. jejuni serotype O:2 ATCC 700819 N-carbamoylputrescine + NH3
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.3.12 0.00533
-
 agmatine pH 7.0, 37°C Campylobacter jejuni subsp. jejuni serotype O:2

General Information

EC Number General Information Comment Organism
3.5.3.12 metabolism the enzyme is involved in the biosynthesis of polyamines Campylobacter jejuni subsp. jejuni serotype O:2