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Literature summary extracted from

  • Larsen, N.; Lin, H.; Wei, R.; Fischbach, M.; Walsh, C.
    Structural characterization of enterobactin hydrolase IroE (2006), Biochemistry, 45, 10184-10190 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.107 expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.1.107 native enzyme and enzyme in complex with diisopropyl fluorophosphonate, sitting drop vapor diffusion method, using 6-10% (w/v) PEG 3350 and 100 mM HEPES (pH 7.5) Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.107 D250A the mutant shows 59% activity with enterobactin and 72% activity with diglucosyl-enterobactin compared to the wild type enzyme Escherichia coli
3.1.1.107 D250N the mutant shows 72% activity with enterobactin and 62% activity with diglucosyl-enterobactin compared to the wild type enzyme Escherichia coli
3.1.1.107 D90A the mutant shows 0.4% activity with enterobactin and 0.56% activity with diglucosyl-enterobactin compared to the wild type enzyme Escherichia coli
3.1.1.107 D90N the mutant shows 1.5% activity with enterobactin and 1.5% activity with diglucosyl-enterobactin compared to the wild type enzyme Escherichia coli
3.1.1.107 E253A the mutant shows 78% activity with enterobactin and 86% activity with diglucosyl-enterobactin compared to the wild type enzyme Escherichia coli
3.1.1.107 E253Q the mutant shows 92% activity with enterobactin and 97% activity with diglucosyl-enterobactin compared to the wild type enzyme Escherichia coli
3.1.1.107 H287A inactive Escherichia coli
3.1.1.107 R130K the mutant shows 0.036% activity with enterobactin and 0.41% activity with diglucosyl-enterobactin compared to the wild type enzyme Escherichia coli
3.1.1.107 R130Q inactive Escherichia coli
3.1.1.107 S189A inactive Escherichia coli
3.1.1.107 S245D the mutant shows 60% activity with enterobactin and 60% activity with diglucosyl-enterobactin compared to the wild type enzyme Escherichia coli
3.1.1.107 S245E the mutant shows 84% activity with enterobactin and 66% activity with diglucosyl-enterobactin compared to the wild type enzyme Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.107 diisopropyl fluorophosphonate
-
 Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.107 periplasm
-
 Escherichia coli
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.107 diglucosyl-enterobactin + H2O Escherichia coli
-
 diglucosyl-(2,3-dihydroxybenzoylserine)3
-
 ?
3.1.1.107 diglucosyl-enterobactin + H2O Escherichia coli CFT073
-
 diglucosyl-(2,3-dihydroxybenzoylserine)3
-
 ?
3.1.1.107 enterobactin + H2O Escherichia coli
-
 N-(2,3-dihydroxybenzoyl)-L-serine trimer
-
 ?
3.1.1.107 enterobactin + H2O Escherichia coli CFT073
-
 N-(2,3-dihydroxybenzoyl)-L-serine trimer
-
 ?
3.1.1.107 monoglucosyl-enterobactin + H2O Escherichia coli
-
 monoglucosyl-(2,3-dihydroxybenzoylserine)3
-
 ?
3.1.1.107 monoglucosyl-enterobactin + H2O Escherichia coli CFT073
-
 monoglucosyl-(2,3-dihydroxybenzoylserine)3
-
 ?
3.1.1.107 salmochelin S4 + H2O Escherichia coli
-
 salmochelin S2
-
 ?
3.1.1.107 salmochelin S4 + H2O Escherichia coli CFT073
-
 salmochelin S2
-
 ?
3.1.1.107 triglucosyl-enterobactin + H2O Escherichia coli
-
 triglucosyl-(2,3-dihydroxybenzoylserine)3
-
 ?
3.1.1.107 triglucosyl-enterobactin + H2O Escherichia coli CFT073
-
 triglucosyl-(2,3-dihydroxybenzoylserine)3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.107 Escherichia coli A0A0H2V871
-
-
3.1.1.107 Escherichia coli CFT073 A0A0H2V871
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.107 Ni-NTA column chromatography and Superdex S200 gel filtration Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.107 diglucosyl-enterobactin + H2O
-
 Escherichia coli diglucosyl-(2,3-dihydroxybenzoylserine)3
-
 ?
3.1.1.107 diglucosyl-enterobactin + H2O
-
 Escherichia coli CFT073 diglucosyl-(2,3-dihydroxybenzoylserine)3
-
 ?
3.1.1.107 enterobactin + H2O
-
 Escherichia coli N-(2,3-dihydroxybenzoyl)-L-serine trimer
-
 ?
3.1.1.107 enterobactin + H2O
-
 Escherichia coli CFT073 N-(2,3-dihydroxybenzoyl)-L-serine trimer
-
 ?
3.1.1.107 monoglucosyl-enterobactin + H2O
-
 Escherichia coli monoglucosyl-(2,3-dihydroxybenzoylserine)3
-
 ?
3.1.1.107 monoglucosyl-enterobactin + H2O
-
 Escherichia coli CFT073 monoglucosyl-(2,3-dihydroxybenzoylserine)3
-
 ?
3.1.1.107 salmochelin S4 + H2O
-
 Escherichia coli salmochelin S2
-
 ?
3.1.1.107 salmochelin S4 + H2O
-
 Escherichia coli CFT073 salmochelin S2
-
 ?
3.1.1.107 triglucosyl-enterobactin + H2O
-
 Escherichia coli triglucosyl-(2,3-dihydroxybenzoylserine)3
-
 ?
3.1.1.107 triglucosyl-enterobactin + H2O
-
 Escherichia coli CFT073 triglucosyl-(2,3-dihydroxybenzoylserine)3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.107 monomer 1 * 30800, calculated from amino acid sequence Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
3.1.1.107 enterobactin hydrolase
-
 Escherichia coli
3.1.1.107 iroE
-
 Escherichia coli