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Literature summary extracted from

  • Soares, C.A.; Knuckley, B.
    Mechanistic studies of the agmatine deiminase from Listeria monocytogenes (2016), Biochem. J., 473, 1553-1561 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.3.12 expression in Escherichia coli BL21(DE3) Listeria monocytogenes serotype 1/2a

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.3.12 2-chloroacetamidine
-
 Listeria monocytogenes serotype 1/2a
3.5.3.12 iodoacetamide
-
 Listeria monocytogenes serotype 1/2a
3.5.3.12 N-(4-aminobutyl)-2-chloro-ethanimidamide
-
 Listeria monocytogenes serotype 1/2a
3.5.3.12 N-(4-aminobutyl)-2-fluoro-ethanimidamide
-
 Listeria monocytogenes serotype 1/2a

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.3.12 0.33
-
 agmatine pH 7.5, 25°C Listeria monocytogenes serotype 1/2a

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.3.12 agmatine + H2O Listeria monocytogenes serotype 1/2a the ability of Listeria monocytogenes to survive in extreme environments (i.e. low temperatures and pH) may arise, at least in part, from agmatine catabolism via the agmatine deiminase system. This catabolic pathway utilizes an agmatine deiminase to hydrolyse agmatine into N-carbamoylputrescine, with concomitant release of ammonia, which increases the pH, thus mitigating the ill effects of the acidic environment N-carbamoylputrescine + NH3
-
 ?
3.5.3.12 agmatine + H2O Listeria monocytogenes serotype 1/2a 10403S the ability of Listeria monocytogenes to survive in extreme environments (i.e. low temperatures and pH) may arise, at least in part, from agmatine catabolism via the agmatine deiminase system. This catabolic pathway utilizes an agmatine deiminase to hydrolyse agmatine into N-carbamoylputrescine, with concomitant release of ammonia, which increases the pH, thus mitigating the ill effects of the acidic environment N-carbamoylputrescine + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.12 Listeria monocytogenes serotype 1/2a A0A0H3G8U9
-
-
3.5.3.12 Listeria monocytogenes serotype 1/2a 10403S A0A0H3G8U9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.3.12
-
 Listeria monocytogenes serotype 1/2a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.12 agmatine + H2O
-
 Listeria monocytogenes serotype 1/2a N-carbamoylputrescine + NH3
-
 ?
3.5.3.12 agmatine + H2O the ability of Listeria monocytogenes to survive in extreme environments (i.e. low temperatures and pH) may arise, at least in part, from agmatine catabolism via the agmatine deiminase system. This catabolic pathway utilizes an agmatine deiminase to hydrolyse agmatine into N-carbamoylputrescine, with concomitant release of ammonia, which increases the pH, thus mitigating the ill effects of the acidic environment Listeria monocytogenes serotype 1/2a N-carbamoylputrescine + NH3
-
 ?
3.5.3.12 agmatine + H2O
-
 Listeria monocytogenes serotype 1/2a 10403S N-carbamoylputrescine + NH3
-
 ?
3.5.3.12 agmatine + H2O the ability of Listeria monocytogenes to survive in extreme environments (i.e. low temperatures and pH) may arise, at least in part, from agmatine catabolism via the agmatine deiminase system. This catabolic pathway utilizes an agmatine deiminase to hydrolyse agmatine into N-carbamoylputrescine, with concomitant release of ammonia, which increases the pH, thus mitigating the ill effects of the acidic environment Listeria monocytogenes serotype 1/2a 10403S N-carbamoylputrescine + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.3.12 ? x * 46800, SDS-PAGE Listeria monocytogenes serotype 1/2a

Synonyms

EC Number Synonyms Comment Organism
3.5.3.12 AgD
-
 Listeria monocytogenes serotype 1/2a

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.3.12 0.517
-
 agmatine pH 7.5, 25°C Listeria monocytogenes serotype 1/2a

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.3.12 7.5
-
-
 Listeria monocytogenes serotype 1/2a

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.3.12 5.5 9.5 pH 5.5: about 60% of maximal activity, pH 9.5: about 65% of maximal activity Listeria monocytogenes serotype 1/2a

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.5.3.12 0.0785
-
 pH 7.5, 25°C Listeria monocytogenes serotype 1/2a N-(4-aminobutyl)-2-chloro-ethanimidamide
3.5.3.12 0.146
-
 pH 7.5, 25°C Listeria monocytogenes serotype 1/2a N-(4-aminobutyl)-2-fluoro-ethanimidamide
3.5.3.12 221
-
 pH 7.5, 25°C Listeria monocytogenes serotype 1/2a 2-chloroacetamidine

General Information

EC Number General Information Comment Organism
3.5.3.12 metabolism the ability of Listeria monocytogenes to survive in extreme environments (i.e. low temperatures and pH) may arise, at least in part, from agmatine catabolism via the agmatine deiminase system. This catabolic pathway utilizes an agmatine deiminase to hydrolyse agmatine into N-carbamoylputrescine, with concomitant release of ammonia, which increases the pH, thus mitigating the ill effects of the acidic environment Listeria monocytogenes serotype 1/2a

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.3.12 1.57
-
 agmatine pH 7.5, 25°C Listeria monocytogenes serotype 1/2a