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Literature summary extracted from

  • Bazaraa, W.; Alian, A.; El-Shimi, N.; Mohamed, R.
    Purification and characterization of extracellular glutaminase from Aspergillus oryzae NRRL 32567 (2016), Biocatal. Agricult. Biotechnol., 6, 76-81 .
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.2 NaCl 2.5%, 130% of initial activity, 10%, 109% of initial activity Aspergillus oryzae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.2 Fe2+ 0.1 mM, 55% of initial activity Aspergillus oryzae
3.5.1.2 Fe3+ 0.1 mM, 45% of initial activity Aspergillus oryzae
3.5.1.2 Mn2+ 0.1 mM, 75% of initial activity Aspergillus oryzae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.2 4.5
-
 L-glutamine pH 7.0, 37°C Aspergillus oryzae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.2 extracellular
-
 Aspergillus oryzae
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.2 Aspergillus oryzae
-
-
-
3.5.1.2 Aspergillus oryzae NRRL 32567
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.2
-
 Aspergillus oryzae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.2 51
-
 pH 7.0, 37°C Aspergillus oryzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.2 D-glutamine + H2O
-
 Aspergillus oryzae D-glutamate + NH3
-
 ?
3.5.1.2 D-glutamine + H2O
-
 Aspergillus oryzae NRRL 32567 D-glutamate + NH3
-
 ?
3.5.1.2 L-asparagine + H2O reaction of EC 3.5.1.1 Aspergillus oryzae L-aspartate + NH3
-
 ?
3.5.1.2 L-asparagine + H2O reaction of EC 3.5.1.1 Aspergillus oryzae NRRL 32567 L-aspartate + NH3
-
 ?
3.5.1.2 L-glutamine + H2O
-
 Aspergillus oryzae L-glutamate + NH3
-
 ?
3.5.1.2 L-glutamine + H2O
-
 Aspergillus oryzae NRRL 32567 L-glutamate + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.2 ? x * 68000, SDS-PAGE Aspergillus oryzae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.2 30 40
-
 Aspergillus oryzae

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.2 25
-
 40% of maximum activity Aspergillus oryzae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.2 30
-
 stable for at least 2 h Aspergillus oryzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.2 7
-
-
 Aspergillus oryzae