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Literature summary extracted from

  • Afshari, E.; Amini-Bayat, Z.; Hosseinkhani, S.; Bakhtiari, N.
    Cloning, expression and purification of pseudomonas putida ATCC12633 creatinase (2017), Avicenna J. Med. Biotechnol., 9, 169-175 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.5.3.3 diagnostics recombinant production of creatinase for using in clinical measurement of serum or urine creatinine Pseudomonas putida

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.3.3
-
 Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.3.3 45000
-
 SDS-PAGE Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.3 Pseudomonas putida
-
-
-
3.5.3.3 Pseudomonas putida ATCC 12633
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.3.3
-
 Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.3.3 26.54
-
 pH and temperature not specified in the publication Pseudomonas putida

Subunits

EC Number Subunits Comment Organism
3.5.3.3 ? x * 45000, SDS-PAGE Pseudomonas putida

General Information

EC Number General Information Comment Organism
3.5.3.3 metabolism the enzyme degrades creatinine in bacteria and provides a source of carbon and nitrogen Pseudomonas putida