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Literature summary extracted from

  • Basnakian, A.G.; Ueda, N.; Hong, X.; Galitovsky, V.E.; Yin, X.; Shah, S.V.
    Ceramide synthase is essential for endonuclease-mediated death of renal tubular epithelial cells induced by hypoxia-reoxygenation (2005), Am. J. Physiol., 288, F308-F314 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.24 additional information exposure of cells to hypoxia-reoxygenation results in a significant increase in ceramide synthase activity Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.24 fumonisin B1 specific ceramide synthase inhibitor, suppresses hypoxia-reoxygenation-induced ceramide generation and provides protection against hypoxia-reoxygenation-induced EndoG release, DNA fragmentation, and cell death at 0.025-0.1 mM Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.24 Mg2+ activates Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.24 sphinganine + palmitoyl-CoA Rattus norvegicus
-
 N-palmitoyldihydrosphingosine + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.24 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.24 NRK-52E cell
-
 Rattus norvegicus
-
2.3.1.24 renal tubule epithelium
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.24 sphinganine + palmitoyl-CoA
-
 Rattus norvegicus N-palmitoyldihydrosphingosine + CoA
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.24 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.24 7.4
-
 assay at Rattus norvegicus

General Information

EC Number General Information Comment Organism
2.3.1.24 malfunction ceramide synthase-specific inhibitor fumonisin B1 suppresses hypoxia-reoxygenation-induced ceramide generation and provides protection against hypoxia-reoxygenation-induced EndoG release, DNA fragmentation, and cell death Rattus norvegicus
2.3.1.24 physiological function ceramide plays a role in the cell signaling pathway involved in apoptosis. Ceramide synthase is essential for endonuclease-mediated death of renal tubular epithelial cells induced by hypoxia reoxygenation. Mainly, enhanced ceramide generation is the result of hydrolysis of sphingomyelin by sphingomyelinases. Role of ceramide synthase in enhanced ceramide generation, overview. Exposure of cells to hypoxia-reoxygenation results in a significant increase in ceramide synthase activity without any significant change in acid or neutral sphingomyelinase. Ceramide synthase-dependent ceramide generation is a key modulator of EndoG-mediated cytotoxicity in hypoxia-reoxygenation injury to renal tubular epithelial cells Rattus norvegicus