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Literature summary extracted from

  • Niyonzima, F.N.; More, S.S.
    Purification and characterization of detergent-compatible protease from Aspergillus terreus gr (2015), 3 Biotech, 5, 61-70 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.21.63 additional information no effect by H2O2 and NaClO Aspergillus terreus
3.4.21.63 SDS
-
 Aspergillus terreus
3.4.21.63 Triton X-100
-
 Aspergillus terreus
3.4.21.63 Tween 80
-
 Aspergillus terreus
3.4.23.18 additional information the enzyme activity remains unaltered in the presence of H2O2 and NaClO Aspergillus terreus
3.4.23.18 SDS
-
 Aspergillus terreus
3.4.23.18 Triton-X100
-
 Aspergillus terreus
3.4.23.18 Tween-80
-
 Aspergillus terreus

Application

EC Number Application Comment Organism
3.4.21.63 detergent alkaline proteases are primarily used in the detergent industry. The addition of the protease to the detergent Super wheel improved its blood stain removal Aspergillus terreus

General Stability

EC Number General Stability Organism
3.4.23.18 the protease is 100 % stable and compatible for 2 h at 60°C with all the detergents tested except for Super wheel, and a retention of 83.98, 85.50, 85.57, 87.14 and 89.16 % is recorded after 24 h for Super wheel, More choice, Ariel, Henko and Surf excel, respectively Aspergillus terreus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.63 DTT 9.9% inhibition at 5 mM Aspergillus terreus
3.4.21.63 EDTA 12.7% inhibition at 5 mM Aspergillus terreus
3.4.21.63 Fe3+ 18.9% inhibition at 5 mM Aspergillus terreus
3.4.21.63 Hg2+ 25.2% inhibition at 5 mM Aspergillus terreus
3.4.21.63 iodoacetamide 5.2% inhibition at 5 mM Aspergillus terreus
3.4.21.63 Mn2+ 14.1% inhibition at 5 mM Aspergillus terreus
3.4.21.63 additional information no effect by H2O2 and NaClO Aspergillus terreus
3.4.21.63 N-bromosuccinimide 50.3% inhibition at 5 mM Aspergillus terreus
3.4.21.63 N-diazoacetylnorleucine methyl ester 8.3% inhibition at 5 mM Aspergillus terreus
3.4.21.63 N-ethylmaleimide 11.2% inhibition at 5 mM Aspergillus terreus
3.4.21.63 Na+ 7.5% inhibition at 5 mM Aspergillus terreus
3.4.21.63 PMSF 83.7% inhibition at 5 mM Aspergillus terreus
3.4.21.63 Sodium azide 22.0% inhibition at 5 mM Aspergillus terreus
3.4.21.63 tosyl-L-lysylchloromethyl ketone 6.7% inhibition at 5 mM Aspergillus terreus
3.4.21.63 Urea 4.5% inhibition at 5 mM Aspergillus terreus
3.4.21.63 Zn2+ 10.9% inhibition at 5 mM Aspergillus terreus
3.4.23.18 DAN 91.7% residual activity at 5 mM Aspergillus terreus
3.4.23.18 dithiothreitol 90.1% residual activity at 5 mM Aspergillus terreus
3.4.23.18 EDTA 87.3% residual activity at 5 mM Aspergillus terreus
3.4.23.18 Fe3+ 81.1% residual activity at 10 mM Aspergillus terreus
3.4.23.18 Hg2+ 74.8% residual activity at 10 mM Aspergillus terreus
3.4.23.18 IAA 94.8% residual activity at 5 mM Aspergillus terreus
3.4.23.18 K+ 97.4% residual activity at 10 mM Aspergillus terreus
3.4.23.18 Mn2+ 85.9% residual activity at 10 mM Aspergillus terreus
3.4.23.18 additional information not inhibited by Fe2+ and Cu2+ Aspergillus terreus
3.4.23.18 N-ethylmaleimide 88.8% residual activity at 5 mM Aspergillus terreus
3.4.23.18 Na+ 92.54% residual activity at 10 mM Aspergillus terreus
3.4.23.18 NaN3 78% residual activity at 5 mM Aspergillus terreus
3.4.23.18 NBS 49.7% residual activity at 5 mM Aspergillus terreus
3.4.23.18 phenylmethylsulfonyl fluoride 16.3% residual activity at 5 mM Aspergillus terreus
3.4.23.18 TLCK 93.3% residual activity at 5 mM Aspergillus terreus
3.4.23.18 Urea 95.5% residual activity at 5 mM Aspergillus terreus
3.4.23.18 Zn2+ 89.1% residual activity at 10 mM Aspergillus terreus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.63 additional information
-
 additional information Km for casein is 5.4 mg/ml Aspergillus terreus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.21.63 Ca2+ activates 82.6% at 10 mM Aspergillus terreus
3.4.21.63 Co2+ activates 21.1% at 10 mM Aspergillus terreus
3.4.21.63 Mg2+ activates 52.2% at 10 mM Aspergillus terreus
3.4.21.63 additional information no or poor effects by 10 mM of K+, Ba2+, Cu2+, and Fe2+ Aspergillus terreus
3.4.23.18 Ca2+ 182.6% activity at 10 mM Aspergillus terreus
3.4.23.18 Co2+ 121.1% activity at 10 mM Aspergillus terreus
3.4.23.18 Mg2+ 152.2% activity at 10 mM Aspergillus terreus
3.4.23.18 Na+ 102.88% activity at 10 mM Aspergillus terreus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.63 16000
-
 about, native PAGE Aspergillus terreus

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.63 Aspergillus terreus
-
-
-
3.4.23.18 Aspergillus terreus
-
-
-
3.4.23.18 Aspergillus terreus gr.
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
3.4.23.18 the enzyme is 100 % stable in the presence of bleaching and oxidizing agents Aspergillus terreus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.63 native enzyme 28.6fold by acetone precipitation, freezing of the precipitate for 3 h at -20°C, dissolvation, and lyophilization, followed by concanavalin A lectin affinity chromatography Aspergillus terreus
3.4.23.18 concanavalin A-agarose column chromatography Aspergillus terreus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.63 148.9
-
 purified enzyme, pH 11.0, 40°C Aspergillus terreus

Storage Stability

EC Number Storage Stability Organism
3.4.21.63 the enzyme exhibits high storage stability at 4°C, 28°C, and -20°C Aspergillus terreus
3.4.23.18 4, 7 and 9% of the enzyme's initial activity are lost after 40 days at 4, 28 and -20°C, respectively Aspergillus terreus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.63 Bovine serum albumin + H2O
-
 Aspergillus terreus ?
-
 ?
3.4.21.63 Bovine serum albumin + H2O
-
 Aspergillus terreus gr. ?
-
 ?
3.4.21.63 casein + H2O best substrate Aspergillus terreus ?
-
 ?
3.4.21.63 casein + H2O best substrate Aspergillus terreus gr. ?
-
 ?
3.4.21.63 Gelatin + H2O the active enzyme degrades the copolymerized gelatin with high activity Aspergillus terreus ?
-
 ?
3.4.21.63 Gelatin + H2O the active enzyme degrades the copolymerized gelatin with high activity Aspergillus terreus gr. ?
-
 ?
3.4.21.63 hen egg albumin + H2O
-
 Aspergillus terreus ?
-
 ?
3.4.21.63 hen egg albumin + H2O
-
 Aspergillus terreus gr. ?
-
 ?
3.4.23.18 casein + H2O highest activity Aspergillus terreus ?
-
 ?
3.4.23.18 casein + H2O highest activity Aspergillus terreus gr. ?
-
 ?
3.4.23.18 Gelatin + H2O
-
 Aspergillus terreus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.63 monomer 1 * 16240, SDS-PAGE and LC-MS Aspergillus terreus
3.4.23.18 monomer 1 * 16000, SDS-PAGE Aspergillus terreus

Synonyms

EC Number Synonyms Comment Organism
3.4.21.63 Alkaline protease
-
 Aspergillus terreus
3.4.21.63 Aspergillus terreus protease
-
 Aspergillus terreus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.63 50
-
 assay at Aspergillus terreus
3.4.23.18 50
-
-
 Aspergillus terreus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.63 40 50 purified enzyme, 24 h, completely stable at Aspergillus terreus
3.4.21.63 60 90 purified secreted enzyme, 1 h, completely stable at Aspergillus terreus
3.4.23.18 50 80 the enzyme is almost 100 % stable at 50°C for 24 h. Retention of full activity is seen for over 4, 2, 1 h and 30 min at 50, 60, 70 and 80°C, respectively. However, a loss in relative activity in the range of 1.3 % (50°C) to 19.6 % (at 80°C) is noted after 24 h Aspergillus terreus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.63 11
-
-
 Aspergillus terreus
3.4.23.18 11
-
-
 Aspergillus terreus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.23.18 8 12 the enzyme is active over a broad alkaline pH range of 8.0-12.0 Aspergillus terreus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.21.63 11 12 purified enzyme, 28°C, 24 h, completely stable at Aspergillus terreus
3.4.23.18 8 12 the protease demonstrates excellent stability at pH range 8.0-12.0 for 30 min. The enzyme remains unaltered at pH 11.0 and 12.0 for 24 h at 28°C and a slight decrease in relative activity is seen at pH 10.0 after 24 h Aspergillus terreus