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Literature summary extracted from

  • Gellings, P.S.; McGee, D.J.
    Arcanobacterium haemolyticum phospholipase D enzymatic activity promotes the hemolytic activity of the cholesterol-dependent cytolysin arcanolysin (2018), Toxins, 10, E213 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.41 expressed in Escherichia coli Top10F' cells Arcanobacterium haemolyticum

Protein Variants

EC Number Protein Variants Comment Organism
3.1.4.41 H49A the mutant shows reduced activity compared to the wild type enzyme Arcanobacterium haemolyticum
3.1.4.41 N68A the mutant shows reduced activity compared to the wild type enzyme Arcanobacterium haemolyticum
3.1.4.41 P43A the mutant shows reduced activity compared to the wild type enzyme Arcanobacterium haemolyticum
3.1.4.41 P43A/H49A the mutant shows strongly reduced activity compared to the wild type enzyme Arcanobacterium haemolyticum
3.1.4.41 P43A/Y45A the mutant shows strongly reduced activity compared to the wild type enzyme Arcanobacterium haemolyticum
3.1.4.41 T275A the mutant shows reduced activity compared to the wild type enzyme Arcanobacterium haemolyticum
3.1.4.41 Y45A the mutant shows reduced activity compared to the wild type enzyme Arcanobacterium haemolyticum
3.1.4.41 Y45A/H49A the mutant shows strongly reduced activity compared to the wild type enzyme Arcanobacterium haemolyticum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.41 sphingomyelin + H2O Arcanobacterium haemolyticum
-
 ceramide phosphate + choline
-
 ?
3.1.4.41 sphingomyelin + H2O Arcanobacterium haemolyticum ATCC 9345
-
 ceramide phosphate + choline
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.41 Arcanobacterium haemolyticum C7SZ47
-
-
3.1.4.41 Arcanobacterium haemolyticum ATCC 9345 C7SZ47
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.41 Ni-NTA resin column chromatography Arcanobacterium haemolyticum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.41 sphingomyelin + H2O
-
 Arcanobacterium haemolyticum ceramide phosphate + choline
-
 ?
3.1.4.41 sphingomyelin + H2O
-
 Arcanobacterium haemolyticum ATCC 9345 ceramide phosphate + choline
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.41 ? x * 30000, SDS-PAGE Arcanobacterium haemolyticum

Synonyms

EC Number Synonyms Comment Organism
3.1.4.41 PLD
-
 Arcanobacterium haemolyticum
3.1.4.41 sphingomyelin-specific phospholipase D
-
 Arcanobacterium haemolyticum

General Information

EC Number General Information Comment Organism
3.1.4.41 physiological function the enzyme activity is necessary to promote arcanolysin-mediated hemolysis of erythrocytes in both time- and concentration-dependent manners Arcanobacterium haemolyticum