Literature summary extracted from

Aoyagi, H.; Katakura, Y.; Iwasaki, A.
Production of secretory cutinase by recombinant Saccharomyces cerevisiae protoplasts (2016), SpringerPlus, 5, 1-6 .

Application

EC Number	Application	Comment	Organism
3.1.1.74	synthesis	expression of enzyme in Saccharomyces cerevisiae. Approximately 28% of the cutinase localizes to the cell walls and/or between cell wall and cell membrane. Protoplasts entrapped in a membrane capsule with a low-viscous liquid-core of 1.92 % w/v calcium-alginate in a static culture secrete measurable amounts of cutinase into the broth. The entrapped protoplasts are cultivated in a shake flask at low osmotic pressure without disruption. During 60 h of cultivation, the extracellular cutinase activity of the free protoplasts at 29.3 atm and protoplasts entrapped in the capsule at 17.2 atm are 0.13 and 0.39 U/mL, respectively	Aspergillus oryzae

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.1.1.74	expression in Saccharomyces cerevisiae	Aspergillus oryzae

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.1.1.74	Aspergillus oryzae	P52956	-	-
3.1.1.74	Aspergillus oryzae ATCC 42149	P52956	-	-

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Release 2023.1 (January 2023)