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Literature summary extracted from

  • Cao, X.; Zhu, L.; Hu, Z.; Cronan, J.
    Expression and activity of the BioH esterase of biotin synthesis is independent of genome context (2017), Sci. Rep., 7, 2141 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.85 expression in Escherichia coli Pseudomonas aeruginosa PAO1
3.1.1.85 expression in Escherichia coli Escherichia coli K-12

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.85 pimeloyl-[acyl-carrier protein] methyl ester + H2O Pseudomonas aeruginosa PAO1 involved in biotin biosynthesis pimeloyl-[acyl-carrier protein] + methanol
-
 ?
3.1.1.85 pimeloyl-[acyl-carrier protein] methyl ester + H2O Escherichia coli K-12 involved in biotin biosynthesis pimeloyl-[acyl-carrier protein] + methanol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.85 Escherichia coli K-12 P13001
-
-
3.1.1.85 Pseudomonas aeruginosa PAO1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.85 N-terminal hexahistidine-tagged enzyme Pseudomonas aeruginosa PAO1
3.1.1.85 N-terminal hexahistidine-tagged enzyme Escherichia coli K-12

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.85 adipyl-[acyl-carrier protein] methyl ester + H2O
-
 Pseudomonas aeruginosa PAO1 adipyl-[acyl-carrier protein] + methanol
-
 ?
3.1.1.85 adipyl-[acyl-carrier protein] methyl ester + H2O
-
 Escherichia coli K-12 adipyl-[acyl-carrier protein] + methanol
-
 ?
3.1.1.85 azelayl-[acyl-carrier protein] methyl ester + H2O
-
 Pseudomonas aeruginosa PAO1 azelayl-[acyl-carrier protein] + methanol
-
 ?
3.1.1.85 azelayl-[acyl-carrier protein] methyl ester + H2O
-
 Escherichia coli K-12 azelayl-[acyl-carrier protein] + methanol
-
 ?
3.1.1.85 additional information no activity with succinyl-[acyl-carrier protein] methyl ester Pseudomonas aeruginosa PAO1 ?
-
 ?
3.1.1.85 additional information no activity with succinyl-[acyl-carrier protein] methyl ester Escherichia coli K-12 ?
-
 ?
3.1.1.85 pimeloyl-[acyl-carrier protein] methyl ester + H2O
-
 Pseudomonas aeruginosa PAO1 pimeloyl-[acyl-carrier protein] + methanol
-
 ?
3.1.1.85 pimeloyl-[acyl-carrier protein] methyl ester + H2O
-
 Escherichia coli K-12 pimeloyl-[acyl-carrier protein] + methanol
-
 ?
3.1.1.85 pimeloyl-[acyl-carrier protein] methyl ester + H2O involved in biotin biosynthesis Pseudomonas aeruginosa PAO1 pimeloyl-[acyl-carrier protein] + methanol
-
 ?
3.1.1.85 pimeloyl-[acyl-carrier protein] methyl ester + H2O involved in biotin biosynthesis Escherichia coli K-12 pimeloyl-[acyl-carrier protein] + methanol
-
 ?
3.1.1.85 suberyl-[acyl-carrier protein] methyl ester + H2O
-
 Pseudomonas aeruginosa PAO1 suberyl-[acyl-carrier protein] + methanol
-
 ?
3.1.1.85 suberyl-[acyl-carrier protein] methyl ester + H2O
-
 Escherichia coli K-12 suberyl-[acyl-carrier protein] + methanol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.85 BioH
-
 Pseudomonas aeruginosa PAO1
3.1.1.85 BioH
-
 Escherichia coli K-12

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.85 37
-
 assay at Pseudomonas aeruginosa PAO1
3.1.1.85 37
-
 assay at Escherichia coli K-12

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.85 7
-
 assay at Pseudomonas aeruginosa PAO1
3.1.1.85 7
-
 assay at Escherichia coli K-12

General Information

EC Number General Information Comment Organism
3.1.1.85 metabolism involved in biotin biosynthesis, in Escherichia coli the bioh gene is not located within a biotin synthetic operon and its transcription is not coregulated with the other biotin synthesis genes. Operon-encoded bioH in Pseudomonas aeruginosa is transcribed at higher levels than the freestanding bioH in Escherichia coli Escherichia coli K-12
3.1.1.85 metabolism involved in biotin biosynthesis, in Pseudomonas aeruginosa the bioH gene is within a biotin synthesis operon and its transcription is coregulated with the other biotin operon genes. Operon-encoded bioH in Pseudomonas aeruginosa is transcribed at higher levels than the freestanding bioH in Escherichia coli Pseudomonas aeruginosa PAO1