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Literature summary extracted from

  • Margenat, M.; Labandera, A.M.; Gil, M.; Carrion, F.; Purificacao, M.; Razzera, G.; Portela, M.M.; Obal, G.; Terenzi, H.; Pritsch, O.; Duran, R.; Ferreira, A.M.; Villarino, A.
    New potential eukaryotic substrates of the mycobacterial protein tyrosine phosphatase PtpA hints of a bacterial modulation of macrophage bioenergetics state (2015), Sci. Rep., 5, 8819 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.48 gene ptpA, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.48 D126A site-directed mutagenesis, mutant PtpA D126A behaves similarly to the PtpA wild-type through the whole expression and purification procedure, suggesting that this mutation does not significantly alter the enzyme structure, but there is a small rearrangement in the protein conformation of the mutant. PtpA wild-type and PtpA D126A mutant present similar values of Km , while the kcat for PtpA D126A is 40fold lower compared to wild-type PtpA, mutant D126A is barely active. PtpA D126A is covalently immobilized on a CM5 sensor chip to assess by surface plasmon resonance (SPR) its ability to bind specifically to macrophage components, SPR analysis of protein-protein interaction, overview Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.48 sodium ortho-vanadate
-
 Mycobacterium tuberculosis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.48 [a protein]-tyrosine phosphate + H2O Mycobacterium tuberculosis
-
 [a protein]-tyrosine + phosphate
-
 ?
3.1.3.48 [a protein]-tyrosine phosphate + H2O Mycobacterium tuberculosis H37Rv
-
 [a protein]-tyrosine + phosphate
-
 ?
3.1.3.48 [a protein]-tyrosine phosphate + H2O Mycobacterium tuberculosis ATCC 25618
-
 [a protein]-tyrosine + phosphate
-
 ?
3.1.3.48 [ATP synthase]-tyrosine phosphate + H2O Mycobacterium tuberculosis
-
 [ATP synthase]-tyrosine + phosphate
-
 ?
3.1.3.48 [ATP synthase]-tyrosine phosphate + H2O Mycobacterium tuberculosis H37Rv
-
 [ATP synthase]-tyrosine + phosphate
-
 ?
3.1.3.48 [ATP synthase]-tyrosine phosphate + H2O Mycobacterium tuberculosis ATCC 25618
-
 [ATP synthase]-tyrosine + phosphate
-
 ?
3.1.3.48 [cytosolic 6-phosphofructokinase]-tyrosine phosphate + H2O Mycobacterium tuberculosis
-
 [cytosolic 6-phosphofructokinase]-tyrosine + phosphate
-
 ?
3.1.3.48 [cytosolic 6-phosphofructokinase]-tyrosine phosphate + H2O Mycobacterium tuberculosis H37Rv
-
 [cytosolic 6-phosphofructokinase]-tyrosine + phosphate
-
 ?
3.1.3.48 [cytosolic 6-phosphofructokinase]-tyrosine phosphate + H2O Mycobacterium tuberculosis ATCC 25618
-
 [cytosolic 6-phosphofructokinase]-tyrosine + phosphate
-
 ?
3.1.3.48 [sulfide quinone oxidoreductase]-tyrosine phosphate + H2O Mycobacterium tuberculosis
-
 [sulfide quinone oxidoreductase]-tyrosine + phosphate
-
 ?
3.1.3.48 [sulfide quinone oxidoreductase]-tyrosine phosphate + H2O Mycobacterium tuberculosis H37Rv
-
 [sulfide quinone oxidoreductase]-tyrosine + phosphate
-
 ?
3.1.3.48 [sulfide quinone oxidoreductase]-tyrosine phosphate + H2O Mycobacterium tuberculosis ATCC 25618
-
 [sulfide quinone oxidoreductase]-tyrosine + phosphate
-
 ?
3.1.3.48 [trifunctional enzyme]-tyrosine phosphate + H2O Mycobacterium tuberculosis
-
 [trifunctional enzyme]-tyrosine + phosphate
-
 ?
3.1.3.48 [trifunctional enzyme]-tyrosine phosphate + H2O Mycobacterium tuberculosis H37Rv
-
 [trifunctional enzyme]-tyrosine + phosphate
-
 ?
3.1.3.48 [trifunctional enzyme]-tyrosine phosphate + H2O Mycobacterium tuberculosis ATCC 25618
-
 [trifunctional enzyme]-tyrosine + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.48 Mycobacterium tuberculosis P9WIA1
-
-
3.1.3.48 Mycobacterium tuberculosis ATCC 25618 P9WIA1
-
-
3.1.3.48 Mycobacterium tuberculosis H37Rv P9WIA1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.48 recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Mycobacterium tuberculosis

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.48 [a protein]-tyrosine phosphate + H2O
-
 Mycobacterium tuberculosis [a protein]-tyrosine + phosphate
-
 ?
3.1.3.48 [a protein]-tyrosine phosphate + H2O
-
 Mycobacterium tuberculosis H37Rv [a protein]-tyrosine + phosphate
-
 ?
3.1.3.48 [a protein]-tyrosine phosphate + H2O
-
 Mycobacterium tuberculosis ATCC 25618 [a protein]-tyrosine + phosphate
-
 ?
3.1.3.48 [ATP synthase]-tyrosine phosphate + H2O
-
 Mycobacterium tuberculosis [ATP synthase]-tyrosine + phosphate
-
 ?
3.1.3.48 [ATP synthase]-tyrosine phosphate + H2O
-
 Mycobacterium tuberculosis H37Rv [ATP synthase]-tyrosine + phosphate
-
 ?
3.1.3.48 [ATP synthase]-tyrosine phosphate + H2O
-
 Mycobacterium tuberculosis ATCC 25618 [ATP synthase]-tyrosine + phosphate
-
 ?
3.1.3.48 [cytosolic 6-phosphofructokinase]-tyrosine phosphate + H2O
-
 Mycobacterium tuberculosis [cytosolic 6-phosphofructokinase]-tyrosine + phosphate
-
 ?
3.1.3.48 [cytosolic 6-phosphofructokinase]-tyrosine phosphate + H2O
-
 Mycobacterium tuberculosis H37Rv [cytosolic 6-phosphofructokinase]-tyrosine + phosphate
-
 ?
3.1.3.48 [cytosolic 6-phosphofructokinase]-tyrosine phosphate + H2O
-
 Mycobacterium tuberculosis ATCC 25618 [cytosolic 6-phosphofructokinase]-tyrosine + phosphate
-
 ?
3.1.3.48 [sulfide quinone oxidoreductase]-tyrosine phosphate + H2O
-
 Mycobacterium tuberculosis [sulfide quinone oxidoreductase]-tyrosine + phosphate
-
 ?
3.1.3.48 [sulfide quinone oxidoreductase]-tyrosine phosphate + H2O
-
 Mycobacterium tuberculosis H37Rv [sulfide quinone oxidoreductase]-tyrosine + phosphate
-
 ?
3.1.3.48 [sulfide quinone oxidoreductase]-tyrosine phosphate + H2O
-
 Mycobacterium tuberculosis ATCC 25618 [sulfide quinone oxidoreductase]-tyrosine + phosphate
-
 ?
3.1.3.48 [trifunctional enzyme]-tyrosine phosphate + H2O
-
 Mycobacterium tuberculosis [trifunctional enzyme]-tyrosine + phosphate
-
 ?
3.1.3.48 [trifunctional enzyme]-tyrosine phosphate + H2O also dephosphorylates the human trifunctional enzyme Mycobacterium tuberculosis [trifunctional enzyme]-tyrosine + phosphate
-
 ?
3.1.3.48 [trifunctional enzyme]-tyrosine phosphate + H2O
-
 Mycobacterium tuberculosis H37Rv [trifunctional enzyme]-tyrosine + phosphate
-
 ?
3.1.3.48 [trifunctional enzyme]-tyrosine phosphate + H2O
-
 Mycobacterium tuberculosis ATCC 25618 [trifunctional enzyme]-tyrosine + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.48 probable low molecular weight protein-tyrosine-phosphatase UniProt Mycobacterium tuberculosis
3.1.3.48 protein tyrosine phosphatase
-
 Mycobacterium tuberculosis
3.1.3.48 PtpA
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.48 30
-
 assay at Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.48 2.6
-
 assay at Mycobacterium tuberculosis

General Information

EC Number General Information Comment Organism
3.1.3.48 physiological function the enzyme is active on protein substrates that play a relevant role in cell energy metabolism. PtpA-mediated dephosphorylation may affect pathways involved in cell energy metabolism, particularly the beta oxidation of fatty acids through modulation of TFP activity and/or cell distribution Mycobacterium tuberculosis