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Literature summary extracted from

  • Zhou, J.; Zheng, Y.; Roembke, B.; Robinson, S.; Opoku-Temeng, C.; Sayre, D.; Sintim, H.
    Fluorescent analogs of cyclic and linear dinucleotides as phosphodiesterase and oligoribonuclease activity probes (2017), RSC Adv., 7, 5421-5426 .
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.13.3 EDTA complete inhibition at 50 mM Escherichia coli
3.1.13.3 EDTA complete inhibition at 50 mM Mycolicibacterium smegmatis
3.1.13.3 EDTA complete inhibition at 50 mM Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.13.3 5'-phosphoguanylyl-(3',5')-guanosine + H2O Escherichia coli i.e. pGpG GMP + guanosine
-
 ?
3.1.13.3 5'-phosphoguanylyl-(3',5')-guanosine + H2O Pseudomonas aeruginosa i.e. pGpG GMP + guanosine
-
 ?
3.1.13.3 5'-phosphoguanylyl-(3',5')-guanosine + H2O Mycolicibacterium smegmatis i.e. pGpG GMP + guanosine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.13.3 Escherichia coli P0A784
-
-
3.1.13.3 Mycolicibacterium smegmatis A0A8B4R3D6
-
-
3.1.13.3 Pseudomonas aeruginosa A0A069Q255
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.13.3 1,N6-etheno-2'-dApG + H2O
-
 Escherichia coli 1,N6-etheno-2'-deoxyadenosine + GMP
-
 ?
3.1.13.3 1,N6-etheno-2'-dApG + H2O
-
 Pseudomonas aeruginosa 1,N6-etheno-2'-deoxyadenosine + GMP
-
 ?
3.1.13.3 1,N6-etheno-2'-dApG + H2O
-
 Mycolicibacterium smegmatis 1,N6-etheno-2'-deoxyadenosine + GMP
-
 ?
3.1.13.3 5'-phosphoguanylyl-(3',5')-guanosine + H2O i.e. pGpG Escherichia coli GMP + guanosine
-
 ?
3.1.13.3 5'-phosphoguanylyl-(3',5')-guanosine + H2O i.e. pGpG Pseudomonas aeruginosa GMP + guanosine
-
 ?
3.1.13.3 5'-phosphoguanylyl-(3',5')-guanosine + H2O i.e. pGpG Mycolicibacterium smegmatis GMP + guanosine
-
 ?
3.1.13.3 dApG + H2O
-
 Escherichia coli 2'-deoxyadenosine + GMP
-
 ?
3.1.13.3 dApG + H2O
-
 Pseudomonas aeruginosa 2'-deoxyadenosine + GMP
-
 ?
3.1.13.3 dApG + H2O
-
 Mycolicibacterium smegmatis 2'-deoxyadenosine + GMP
-
 ?
3.1.13.3 additional information poor activity with 4-nitrophenyl phosphate Escherichia coli ?
-
 ?
3.1.13.3 additional information poor activity with 4-nitrophenyl phosphate Pseudomonas aeruginosa ?
-
 ?
3.1.13.3 additional information poor activity with 4-nitrophenyl phosphate Mycolicibacterium smegmatis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.13.3 oligoribonuclease
-
 Escherichia coli
3.1.13.3 oligoribonuclease
-
 Pseudomonas aeruginosa
3.1.13.3 oligoribonuclease
-
 Mycolicibacterium smegmatis
3.1.13.3 ORN
-
 Escherichia coli
3.1.13.3 ORN
-
 Pseudomonas aeruginosa
3.1.13.3 ORN
-
 Mycolicibacterium smegmatis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.13.3 37
-
 assay at Escherichia coli
3.1.13.3 37
-
 assay at Pseudomonas aeruginosa
3.1.13.3 37
-
 assay at Mycolicibacterium smegmatis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.13.3 8
-
 assay at Escherichia coli
3.1.13.3 8
-
 assay at Pseudomonas aeruginosa
3.1.13.3 8
-
 assay at Mycolicibacterium smegmatis

General Information

EC Number General Information Comment Organism
3.1.13.3 physiological function oligoribonuclease (Orn), which had earlier been established as an essential enzyme in most bacteria involved in the recycling of RNA into ribonucleotides, acts as c-di-GMP PDB-B in Pseudomonas aeruginosa cleaving diguanylate into (pGpG), and pGpG then into GMP Pseudomonas aeruginosa