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Literature summary extracted from

  • Riddle, R.; Gibbs, P.; Willson, R.; Benedik, M.
    Purification and properties of 2-hydroxy-6-oxo-6(2'-aminophenyl)hexa-2,4-dienoic acid hydrolase involved in microbial degradation of carbazole (2003), Protein Expr. Purif., 28, 182-189 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.7.1.25 expressed in Escherichia coli Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.7.1.25 0.0046
-
 2-hydroxy-6-oxo-6-(2'-aminophenyl)hexa-2,4-dienoate at pH 7.0 and 60°C Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
3.7.1.25 Pseudomonas sp.
-
-
-
3.7.1.25 Pseudomonas sp. LD2
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.7.1.25 immobilized metal affinity column chromatography Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.7.1.25 2-hydroxy-6-oxo-6-(2'-aminophenyl)hexa-2,4-dienoate + H2O
-
 Pseudomonas sp. 2-hydroxypenta-2,4-dienoate + benzoate
-
 ?
3.7.1.25 2-hydroxy-6-oxo-6-(2'-aminophenyl)hexa-2,4-dienoate + H2O
-
 Pseudomonas sp. LD2 2-hydroxypenta-2,4-dienoate + benzoate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.7.1.25 homodimer
-
 Pseudomonas sp.

Synonyms

EC Number Synonyms Comment Organism
3.7.1.25 2-hydroxy-6-oxo-6(2'-aminophenyl)hexa-2,4-dienoic acid hydrolase
-
 Pseudomonas sp.
3.7.1.25 HOPDA hydrolase
-
 Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.7.1.25 60
-
-
 Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.7.1.25 7
-
-
 Pseudomonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.7.1.25 6.5 10.5 the enzyme exhibits activity between pH 6.5 and 10.5 Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
3.7.1.25 additional information the enzyme has no cofactor requirement Pseudomonas sp.