Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Bernardi, A.V.; de Gouvea, P.F.; Gerolamo, L.E.; Yonamine, D.K.; de Lourdes de Lima Balico, L.; Uyemura, S.A.; Dinamarco, T.M.
    Functional characterization of GH7 endo-1,4-beta-glucanase from Aspergillus fumigatus and its potential industrial application (2018), Protein Expr. Purif., 150, 1-11 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.73 2-mercaptoethanol activation to 132.1% at 5 mM Aspergillus fumigatus
3.2.1.73 DMSO activation to 111.5% at 5 mM Aspergillus fumigatus
3.2.1.73 DTT activation to 165.2% at 5 mM Aspergillus fumigatus
3.2.1.73 EDTA activation to 108.1% at 5 mM Aspergillus fumigatus
3.2.1.73 L-cysteine activation to 152.2% at 5 mM Aspergillus fumigatus
3.2.1.73 Tween 20 activation to 106.2% at 5 mM Aspergillus fumigatus

Application

EC Number Application Comment Organism
3.2.1.73 additional information upon addition to a commercial cellulase cocktail, Af-EGL7 significantly improves sugarcane exploded bagasse (SEB) saccharification, which suggests that enzyme Af-EGL7 has great potential to hydrolyze complex biomass. Af-EGL7 is a promising candidate to enhance enzyme cocktails used in biorefineries such as consolidated bioprocessing Aspergillus fumigatus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.73 gene AFUA_6G01800 or Af-egl7, DNA and amino acid sequnce determination and analysis, sequence comparisons, quantitative RT-PCR exoression analysis, recombinant expression in Escherichia coli strain Rosetta (DE3) pLysS in inclusion bodies, subcloning in Escherichia coli strain DH10beta Aspergillus fumigatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.73 Ag+ complete inhibition at 5 mM Aspergillus fumigatus
3.2.1.73 Cu2+ 32.4% inhibition at 5 mM Aspergillus fumigatus
3.2.1.73 Mn2+ activates by 233% at 5 mM, best at 10 mM, slightly inhibitory at 20 mM Aspergillus fumigatus
3.2.1.73 SDS complete inhibition Aspergillus fumigatus
3.2.1.73 Zn2+ 20.4% inhibition at 5 mM Aspergillus fumigatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.73 additional information
-
additional information Km for carboxymethyl cellulose is 209.7 mg/ml and for barley beta-glucan 113.9 mg/ml in presence of 5 mM Mn2+ Aspergillus fumigatus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.73 extracellular N-terminal signal peptide at residues 1-20 (MDSKRGVVAAVLALLPLVSA), enzyme Af-EGL7 is secreted Aspergillus fumigatus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.73 (NH4)2SO4 activation to 105.5% at 5 mM Aspergillus fumigatus
3.2.1.73 Ca2+ activation to 135.7% at 5 mM Aspergillus fumigatus
3.2.1.73 Co2+ activation to 161.1% at 5 mM Aspergillus fumigatus
3.2.1.73 Fe2+ activation to 128.1% at 5 mM Aspergillus fumigatus
3.2.1.73 KCl activation to 110.4% at 5 mM Aspergillus fumigatus
3.2.1.73 Mg2+ activation to 122.6% at 5 mM Aspergillus fumigatus
3.2.1.73 Mn2+ activates by 233%, best at 10 mM, slightly inhibitory at 20 mM Aspergillus fumigatus
3.2.1.73 NaCl activation to 113.2% at 5 mM Aspergillus fumigatus
3.2.1.73 Ni2+ activation to 114.2% at 5 mM Aspergillus fumigatus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.73 Aspergillus fumigatus Q4WCM9 i.e. Aspergillus fumigatus
-
3.2.1.73 Aspergillus fumigatus Af293 Q4WCM9 i.e. Aspergillus fumigatus
-
3.2.1.73 Aspergillus fumigatus ATCC MYA-4609 Q4WCM9 i.e. Aspergillus fumigatus
-
3.2.1.73 Aspergillus fumigatus CBS 101355 Q4WCM9 i.e. Aspergillus fumigatus
-
3.2.1.73 Aspergillus fumigatus FGSC A1100 Q4WCM9 i.e. Aspergillus fumigatus
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.73 glycoprotein the enzyme has four potential N-glycosylation sites (N96, N201, N205, and N379) that are highly conserved among GH7 endoglucanases from other Aspergillus species Aspergillus fumigatus

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.2.1.73 recombinant enzyme from inclusion bodies by denaturing and dialysis against Tris 10 mM, TCEP 0.1 mM, 0.05 mM EDTA, and 0.06% v/v N-lauroylsarcosine, pH 8.0, and protein refolding in 50 mM Tris, 12.5 mM cyclodextrin, and 1 mM EDTA, pH 7.5 by dialysis Aspergillus fumigatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.73 barley beta-glucan + H2O best substrate, 1% w/v substrate concentration, best substrate Aspergillus fumigatus ?
-
?
3.2.1.73 barley beta-glucan + H2O best substrate, 1% w/v substrate concentration, best substrate Aspergillus fumigatus CBS 101355 ?
-
?
3.2.1.73 barley beta-glucan + H2O best substrate, 1% w/v substrate concentration, best substrate Aspergillus fumigatus ATCC MYA-4609 ?
-
?
3.2.1.73 barley beta-glucan + H2O best substrate, 1% w/v substrate concentration, best substrate Aspergillus fumigatus FGSC A1100 ?
-
?
3.2.1.73 barley beta-glucan + H2O best substrate, 1% w/v substrate concentration, best substrate Aspergillus fumigatus Af293 ?
-
?
3.2.1.73 carboxymethyl cellulose + H2O 1% w/v substrate concentration, low activity Aspergillus fumigatus ?
-
?
3.2.1.73 carboxymethyl cellulose + H2O 1% w/v substrate concentration, low activity Aspergillus fumigatus CBS 101355 ?
-
?
3.2.1.73 carboxymethyl cellulose + H2O 1% w/v substrate concentration, low activity Aspergillus fumigatus ATCC MYA-4609 ?
-
?
3.2.1.73 carboxymethyl cellulose + H2O 1% w/v substrate concentration, low activity Aspergillus fumigatus FGSC A1100 ?
-
?
3.2.1.73 carboxymethyl cellulose + H2O 1% w/v substrate concentration, low activity Aspergillus fumigatus Af293 ?
-
?
3.2.1.73 additional information the enzyme exhibits higher activity toward beta-glucan than toward xyloglucan and carboxymethyl cellulose suggesting that the enzyme corresponds to a beta-1,3-1,4-glucanase. No activity with Avicel and beechwood xylan Aspergillus fumigatus ?
-
?
3.2.1.73 additional information the enzyme exhibits higher activity toward beta-glucan than toward xyloglucan and carboxymethyl cellulose suggesting that the enzyme corresponds to a beta-1,3-1,4-glucanase. No activity with Avicel and beechwood xylan Aspergillus fumigatus CBS 101355 ?
-
?
3.2.1.73 additional information the enzyme exhibits higher activity toward beta-glucan than toward xyloglucan and carboxymethyl cellulose suggesting that the enzyme corresponds to a beta-1,3-1,4-glucanase. No activity with Avicel and beechwood xylan Aspergillus fumigatus ATCC MYA-4609 ?
-
?
3.2.1.73 additional information the enzyme exhibits higher activity toward beta-glucan than toward xyloglucan and carboxymethyl cellulose suggesting that the enzyme corresponds to a beta-1,3-1,4-glucanase. No activity with Avicel and beechwood xylan Aspergillus fumigatus FGSC A1100 ?
-
?
3.2.1.73 additional information the enzyme exhibits higher activity toward beta-glucan than toward xyloglucan and carboxymethyl cellulose suggesting that the enzyme corresponds to a beta-1,3-1,4-glucanase. No activity with Avicel and beechwood xylan Aspergillus fumigatus Af293 ?
-
?
3.2.1.73 sugarcane exploded bagasse + H2O 1% w/v substrate concentration, sugarcane exploded bagasse biomass (SEB). SEB is a highly heterogeneous material that consists of 47.5% cellulose, 9.0% hemicellulose, and 34.3% lignin Aspergillus fumigatus ?
-
?
3.2.1.73 sugarcane exploded bagasse + H2O 1% w/v substrate concentration, sugarcane exploded bagasse biomass (SEB). SEB is a highly heterogeneous material that consists of 47.5% cellulose, 9.0% hemicellulose, and 34.3% lignin Aspergillus fumigatus CBS 101355 ?
-
?
3.2.1.73 sugarcane exploded bagasse + H2O 1% w/v substrate concentration, sugarcane exploded bagasse biomass (SEB). SEB is a highly heterogeneous material that consists of 47.5% cellulose, 9.0% hemicellulose, and 34.3% lignin Aspergillus fumigatus ATCC MYA-4609 ?
-
?
3.2.1.73 sugarcane exploded bagasse + H2O 1% w/v substrate concentration, sugarcane exploded bagasse biomass (SEB). SEB is a highly heterogeneous material that consists of 47.5% cellulose, 9.0% hemicellulose, and 34.3% lignin Aspergillus fumigatus FGSC A1100 ?
-
?
3.2.1.73 sugarcane exploded bagasse + H2O 1% w/v substrate concentration, sugarcane exploded bagasse biomass (SEB). SEB is a highly heterogeneous material that consists of 47.5% cellulose, 9.0% hemicellulose, and 34.3% lignin Aspergillus fumigatus Af293 ?
-
?
3.2.1.73 xyloglucan + H2O high activity, xyloglucan from tamarind seed, 1% w/v substrate concentration Aspergillus fumigatus ?
-
?
3.2.1.73 xyloglucan + H2O high activity, xyloglucan from tamarind seed, 1% w/v substrate concentration Aspergillus fumigatus CBS 101355 ?
-
?
3.2.1.73 xyloglucan + H2O high activity, xyloglucan from tamarind seed, 1% w/v substrate concentration Aspergillus fumigatus ATCC MYA-4609 ?
-
?
3.2.1.73 xyloglucan + H2O high activity, xyloglucan from tamarind seed, 1% w/v substrate concentration Aspergillus fumigatus FGSC A1100 ?
-
?
3.2.1.73 xyloglucan + H2O high activity, xyloglucan from tamarind seed, 1% w/v substrate concentration Aspergillus fumigatus Af293 ?
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.73 ? x * 52000, recombinant enzyme, SDS-PAGE, x * 48000, about, sequence calculation Aspergillus fumigatus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.73 Af-EGL7
-
Aspergillus fumigatus
3.2.1.73 Afu6g01800
-
Aspergillus fumigatus
3.2.1.73 beta-1,3-1,4-glucanase
-
Aspergillus fumigatus
3.2.1.73 GH7 endo-1,4-beta-glucanase
-
Aspergillus fumigatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.73 55
-
-
Aspergillus fumigatus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.73 30 80 40% of maximal activity at 30°C, 80% at 45°C and 60°C, about 35% at 70°C, and 20% at 80°C, profile overview Aspergillus fumigatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.73 30 60 completely stable up to 50°C for 1 h and loss of 10% activity after 48 h at 50°C, 50% activity remaing at 55°C after 30 min and inactivation after 5 h at 55°C, rapid inactivation at 60°C Aspergillus fumigatus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.73 43.32
-
carboxymethyl cellulose pH 5.0, 55°C, recombinant enzyme Aspergillus fumigatus
3.2.1.73 159.9
-
Barley beta-glucan pH 5.0, 55°C, recombinant enzyme Aspergillus fumigatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.73 5
-
-
Aspergillus fumigatus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.73 3 6.5 activity range, over 50% of maximal activity at pH 4.0-5.5, profile overview Aspergillus fumigatus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.73 3 8 completely stable up to pH 6.0, 40% activity remaining at pH 7.0, inactivation at pH 8.0 Aspergillus fumigatus

Expression

EC Number Organism Comment Expression
3.2.1.73 Aspergillus fumigatus substrate sugarcane exploded bagasse (SEB), induces Af-egl7 by 2500fold up

General Information

EC Number General Information Comment Organism
3.2.1.73 evolution the enzyme belongs to the glycosyl hydrolase family 7, GH7 Aspergillus fumigatus
3.2.1.73 additional information residues 424-460 from the carbohydrate-binding domain, CBM1 domain Aspergillus fumigatus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.73 additional information
-
additional information Km for carboxymethyl cellulose is 0.207 mg/ml/s and for barley beta-glucan 1.404 mg/ml/s in presence of 5 mM Mn2+ Aspergillus fumigatus