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Literature summary extracted from

  • Zhao, L.; Xie, Y.; Chen, L.; Xu, X.; Zhao, C.; Cheng, F.
    Efficient biotransformation of isoeugenol to vanillin in recombinant strains of Escherichia coli by using engineered isoeugenol monooxygenase and sol-gel chitosan membrane (2018), Process Biochem., 71, 76-81 .
No PubMed abstract available

Application

EC Number Application Comment Organism
1.13.11.88 synthesis upon expression of mutant F281Q in Escherichia coli and employing sol-gel chitosan membrane for removing the produced vanillin from the biotransformation system, under the optimal conditions (pH 8.5, 30°C, 180 rpm, 0.5 g wet cells, 0.1 g chitosan membrane), vanillin concentrations reach 4.5 g/l after about 40 h Pseudomonas sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.88 expression in Escherichia coli Pseudomonas sp.

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.13.11.88 homology modeling of structure and docking of substrate isoeugenol to the active site Pseudomonas sp.

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.88 F281Q mutant displays the highest conversion of isoeugenol Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.88 0.145
-
 isoeugenol wild-type, 30°C, pH not specified in the publication Pseudomonas sp.
1.13.11.88 0.15
-
 isoeugenol mutant F281Q, 30°C, pH not specified in the publication Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.88 Pseudomonas sp. A0A384ZRB9
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.88 4.6
-
 wild-type, 30°C, pH not specified in the publication Pseudomonas sp.
1.13.11.88 7.5
-
 mutant F281Q, 30°C, pH not specified in the publication Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.88 isoeugenol + O2
-
 Pseudomonas sp. vanillin + acetaldehyde
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.88 ? x * 53000, calculated from sequence Pseudomonas sp.

Synonyms

EC Number Synonyms Comment Organism
1.13.11.88 Iem720
-
 Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.88 30
-
-
 Pseudomonas sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.88 4.21
-
 isoeugenol wild-type, 30°C, pH not specified in the publication Pseudomonas sp.
1.13.11.88 7.58
-
 isoeugenol mutant F281Q, 30°C, pH not specified in the publication Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.88 8.5
-
-
 Pseudomonas sp.