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Literature summary extracted from

  • Karboune, S.; Appanah, N.; Khodaei, N.; Tian, F.
    Enzymatic synthesis of fructooligosaccharides from sucrose by endo-inulinase-catalyzed transfructosylation reaction in biphasic systems (2018), Process Biochem., 69, 82-91 .
No PubMed abstract available

Application

EC Number Application Comment Organism
3.2.1.7 synthesis enzymatic synthesis of fructooligosaccharides (FOS) from sucrose by endo-inulinase-catalyzed transfructosylation reaction in biphasic systems, production of FOS from sucrose by commercial inulinase from Aspergillus niger Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.7 Aspergillus niger O74641
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Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.7 the commercial preparation of endoinulase is further purified 8.2fold by anion exchange chromatography to exclude any exoinulase activity Aspergillus niger

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.7 commercial preparation
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 Aspergillus niger
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Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.7 additional information
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 specific activity of the crude commercial inulinase enzyme is estimated to be 1.2596 mmol/mg protein/min, while that of the purified endo-inulinase enzyme is found to be 10.372 mmol/mg protein/min Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.7 additional information enzymatic synthesis of fructooligosaccharides (FOS) from sucrose by endo-inulinase-catalyzed transfructosylation reaction of sucrose in biphasic systems, production of FOS from sucrose by commercial inulinase from Aspergillus niger, modeling, overview. With the use of maple syrup 66°Bx-based biphasic medium, kestose, nystose, and fructosyl-nystose are all produced during the first 16 h, with a relative proportion of 78.79%, 20.34%, and 0.86%, respectively Aspergillus niger ?
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 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.7 endo-inulinase
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 Aspergillus niger
3.2.1.7 inu1
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 Aspergillus niger

General Information

EC Number General Information Comment Organism
3.2.1.7 additional information reaction selectivity of transfructosylation vs. hydrolysis of endo-inulinase from Aspergillus niger in selected biphasic media, overview Aspergillus niger