Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Onwueme, K.C.; Ferreras, J.A.; Buglino, J.; Lima, C.D.; Quadri, L.E.
    Mycobacterial polyketide-associated proteins are acyltransferases proof of principle with Mycobacterium tuberculosis PapA5 (2004), Proc. Natl. Acad. Sci. USA, 101, 4608-4613 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.282 D128A mutation in HHx3DGx13Y motif, loss of activity Mycobacterium tuberculosis
2.3.1.282 H123A about 1.7- to 2.3fold decrease in ester formation Mycobacterium tuberculosis
2.3.1.282 H124A mutation in HHx3DGx13Y motif, loss of activity Mycobacterium tuberculosis
2.3.1.282 Y143F about 1.9fold decrease in ester formation Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.282 octan-1-ol substrate inhibition Mycobacterium tuberculosis
2.3.1.282 palmitoyl-CoA substrate inhibition Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.282 0.004
-
 palmitoyl-CoA pH 6.5, 387°C Mycobacterium tuberculosis
2.3.1.282 0.5
-
 octan-1-ol pH 6.5, 387°C Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.282 Mycobacterium tuberculosis P9WIN5
-
-
2.3.1.282 Mycobacterium tuberculosis ATCC 25618 P9WIN5
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.282 (R)-octan-2-ol + palmitoyl-CoA
-
 Mycobacterium tuberculosis (R)-2-octanyl palmitate + CoA
-
 ?
2.3.1.282 (R)-octan-2-ol + palmitoyl-CoA
-
 Mycobacterium tuberculosis ATCC 25618 (R)-2-octanyl palmitate + CoA
-
 ?
2.3.1.282 (R,S)-octan-2-ol + palmitoyl-CoA
-
 Mycobacterium tuberculosis (R,S)-2-octanyl palmitate + CoA
-
 ?
2.3.1.282 (R,S)-octan-2-ol + palmitoyl-CoA
-
 Mycobacterium tuberculosis ATCC 25618 (R,S)-2-octanyl palmitate + CoA
-
 ?
2.3.1.282 decan-1-ol + palmitoyl-CoA
-
 Mycobacterium tuberculosis 1-decyl palmitate + CoA
-
 ?
2.3.1.282 decan-1-ol + palmitoyl-CoA
-
 Mycobacterium tuberculosis ATCC 25618 1-decyl palmitate + CoA
-
 ?
2.3.1.282 hexadecanol + palmitoyl-CoA
-
 Mycobacterium tuberculosis hexadecyl palmitate + CoA
-
 ?
2.3.1.282 hexadecanol + palmitoyl-CoA
-
 Mycobacterium tuberculosis ATCC 25618 hexadecyl palmitate + CoA
-
 ?
2.3.1.282 additional information enzyme shows a preference for saturated medium chain alcohols, with 1-octanol displaying the highest ester formation stoichiometry. 20- and 5fold more ester is formed with (R)-(-)2-octanol and (S)-(-)1,2-decanediol, respectively, than with their corresponding enantiomers Mycobacterium tuberculosis ?
-
 ?
2.3.1.282 additional information enzyme shows a preference for saturated medium chain alcohols, with 1-octanol displaying the highest ester formation stoichiometry. 20- and 5fold more ester is formed with (R)-(-)2-octanol and (S)-(-)1,2-decanediol, respectively, than with their corresponding enantiomers Mycobacterium tuberculosis ATCC 25618 ?
-
 ?
2.3.1.282 nonan-1-ol + palmitoyl-CoA
-
 Mycobacterium tuberculosis 1-nonyl palmitate + CoA
-
 ?
2.3.1.282 octan-1-ol + elaidoyl-CoA
-
 Mycobacterium tuberculosis 1-octyl elaidoate + CoA
-
 ?
2.3.1.282 octan-1-ol + heptadecanoyl-CoA
-
 Mycobacterium tuberculosis 1-octyl heptadecanoate + CoA
-
 ?
2.3.1.282 octan-1-ol + lauroyl-CoA
-
 Mycobacterium tuberculosis 1-octyl laurate + CoA
-
 ?
2.3.1.282 octan-1-ol + oleoyl-CoA
-
 Mycobacterium tuberculosis 1-octyl oleate + CoA
-
 ?
2.3.1.282 octan-1-ol + palmitoyl-CoA
-
 Mycobacterium tuberculosis 1-octyl palmitate + CoA
-
 ?
2.3.1.282 octan-1-ol + stearoyl-CoA
-
 Mycobacterium tuberculosis 1-octyl stearate + CoA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.282 papA5
-
 Mycobacterium tuberculosis
2.3.1.282 polyketide-associated protein A5
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.282 0.0004
-
 octan-1-ol pH 6.5, 387°C Mycobacterium tuberculosis
2.3.1.282 0.00045
-
 palmitoyl-CoA pH 6.5, 387°C Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.282 6.5
-
-
 Mycobacterium tuberculosis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.1.282 0.0096
-
 palmitoyl-CoA pH 6.5, 37°C Mycobacterium tuberculosis
2.3.1.282 3
-
 octan-1-ol pH 6.5, 37°C Mycobacterium tuberculosis

General Information

EC Number General Information Comment Organism
2.3.1.282 physiological function PapA5 catalyzes diesterification of phthiocerol and phthiodiolone with mycocerosate. Presence of PapA5 is required for synthesis of phthiocerol and phthiodiolone dimycocerosate esters Mycobacterium tuberculosis