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Literature summary extracted from

  • Parsons, X.H.; Garcia, S.N.; Pillus, L.; Kadonaga, J.T.
    Histone deacetylation by Sir2 generates a transcriptionally repressed nucleoprotein complex (2003), Proc. Natl. Acad. Sci. USA, 100, 1609-1614 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.286 expressed in Sf9 insect cells Drosophila melanogaster

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.286 coumermycin A1 completely inhibited by 0.2 mM Drosophila melanogaster

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.286 hyperacetylated histone-DNA complex + NAD+ + H2O Drosophila melanogaster
-
deacetylated nuclease-resistant histone-DNA complex + ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.286 Drosophila melanogaster Q9VK34
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.286 Ni-affinity column chromatography and Superdex S200 gel filtration Drosophila melanogaster

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.286 hyperacetylated histone-DNA complex + NAD+ + H2O
-
Drosophila melanogaster deacetylated nuclease-resistant histone-DNA complex + ?
-
?
2.3.1.286 [histone H2B]-N6-acetyl-L-lysine20 + NAD+ + H2O
-
Drosophila melanogaster [histone H2B]-L-lysine20 + 2''-O-acetyl-ADP-D-ribose + nicotinamide
-
?
2.3.1.286 [histone H2B]-N6-acetyl-L-lysine5 + NAD+ + H2O
-
Drosophila melanogaster [histone H2B]-L-lysine5 + 2''-O-acetyl-ADP-D-ribose + nicotinamide
-
?
2.3.1.286 [histone H3]-N6-acetyl-L-lysine14 + NAD+ + H2O
-
Drosophila melanogaster [histone H3]-L-lysine14 + 2''-O-acetyl-ADP-D-ribose + nicotinamide
-
?
2.3.1.286 [histone H3]-N6-acetyl-L-lysine18 + NAD+ + H2O
-
Drosophila melanogaster [histone H3]-L-lysine18 + 2''-O-acetyl-ADP-D-ribose + nicotinamide
-
?
2.3.1.286 [histone H3]-N6-acetyl-L-lysine23 + NAD+ + H2O
-
Drosophila melanogaster [histone H3]-L-lysine23 + 2''-O-acetyl-ADP-D-ribose + nicotinamide
-
?
2.3.1.286 [histone H3]-N6-acetyl-L-lysine9 + NAD+ + H2O
-
Drosophila melanogaster [histone H3]-L-lysine9 + 2''-O-acetyl-ADP-D-ribose + nicotinamide
-
?
2.3.1.286 [histone H4]-N6-acetyl-L-lysine12 + NAD+ + H2O
-
Drosophila melanogaster [histone H4]-L-lysine12 + 2''-O-acetyl-ADP-D-ribose + nicotinamide
-
?
2.3.1.286 [histone H4]-N6-acetyl-L-lysine16 + NAD+ + H2O
-
Drosophila melanogaster [histone H4]-L-lysine16 + 2''-O-acetyl-ADP-D-ribose + nicotinamide
-
?
2.3.1.286 [histone H4]-N6-acetyl-L-lysine5 + NAD+ + H2O
-
Drosophila melanogaster [histone H4]-L-lysine5 + 2''-O-acetyl-ADP-D-ribose + nicotinamide
-
?
2.3.1.286 [histone H4]-N6-acetyl-L-lysine8 + NAD+ + H2O weak activity Drosophila melanogaster [histone H4]-L-lysine8 + 2''-O-acetyl-ADP-D-ribose + nicotinamide
-
?

Subunits

EC Number Subunits Comment Organism
2.3.1.286 ? x * 116000, SDS-PAGE Drosophila melanogaster

Synonyms

EC Number Synonyms Comment Organism
2.3.1.286 NAD-dependent histone deacetylase
-
Drosophila melanogaster
2.3.1.286 Sir2
-
Drosophila melanogaster
2.3.1.286 SIRT1
-
Drosophila melanogaster

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.286 NAD+
-
Drosophila melanogaster

General Information

EC Number General Information Comment Organism
2.3.1.286 physiological function the enzyme does not repress transcription with either naked DNA templates or chromatin assembled from native (and mostly unacetylated) histones Drosophila melanogaster