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Literature summary extracted from

  • Li, T.; Wu, Y.; He, J.
    Heterologous expression, characterization and application of a new beta-xylosidase identified in solventogenic Clostridium sp. strain BOH3 (2018), Proc. Biochem., 67, 99-104 .
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.37 SDS 1 mM, enhances activity to 118% Clostridium sp. BOH3
3.2.1.37 Tween-80 1 mM, enhances activity to 120% Clostridium sp. BOH3

Application

EC Number Application Comment Organism
3.2.1.37 biofuel production application of this recombinant beta-xylosidase together with xylanase improves xylan hydrolysis efficiency, thus leading to increased biofuels productivity Clostridium sp. BOH3

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.37 the recombinant enzyme is expressed in Escherichia coli Clostridium sp. BOH3

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.37 4-Aminobenzoate 1 mM, 20% inhibition Clostridium sp. BOH3
3.2.1.37 Ag+ 0.1 mM, complete loss of activity Clostridium sp. BOH3
3.2.1.37 Cu2+ 1 mM, 10% loss of activity Clostridium sp. BOH3
3.2.1.37 EDTA 0.1 mM, 60% loss of activity. 1 mM, 10% loss of activity Clostridium sp. BOH3
3.2.1.37 Fe2+ 1 mM, 30% loss of activity Clostridium sp. BOH3
3.2.1.37 Hg2+ 0.1 mM, complete loss of activity Clostridium sp. BOH3
3.2.1.37 Zn2+ 0.1 mM, 1.6fold activation. 1 mM, 25% loss of activity Clostridium sp. BOH3

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.37 Ca2+ 0.1 mM, 1.2fold activation. 1 mM, 1.5fold activation Clostridium sp. BOH3
3.2.1.37 Co2+ 0.1 mM, 1.2fold activation. 1 mM, 1.4fold activation Clostridium sp. BOH3
3.2.1.37 Mn2+ 0.1 mM, 1.3fold activation Clostridium sp. BOH3
3.2.1.37 Zn2+ 0.1 mM, 1.6fold activation. 1 mM, 25% loss of activity Clostridium sp. BOH3

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.37 Clostridium sp. BOH3 A0A024A6V9
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.37 4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Clostridium sp. BOH3 4-nitrophenol + beta-D-xylopyranose
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.37 40
-
-
 Clostridium sp. BOH3

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.37 5 6
-
 Clostridium sp. BOH3