EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.3.8 | gene appA or phyS, cloning from library, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of extracellular His-tagged enzyme in Escherichia coli strain BL21(DE3) as nonglycosylated protein and of extracellular nontagged enzyme in Pichia pastoris strain GS115(his4) under control of the AOX1 promoter as glycosylated protein | Shigella sp. CD2 |
3.1.3.26 | gene appA or phyS, cloning from library, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of extracellular His-tagged enzyme in Escherichia coli strain BL21(DE3) as nonglycosylated protein and of extracellular nontagged enzyme in Pichia pastoris strain GS115(his4) under control of the AOX1 promoter as glycosylated protein | Shigella sp. CD2 |
3.1.3.72 | gene appA or phyS, cloning from library, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of extracellular His-tagged enzyme in Escherichia coli strain BL21(DE3) as non-glycosylated protein and of extracellular nontagged enzyme in Pichia pastoris strain GS115(his4) under control of the AOX1 promoter as glycosylated protein | Shigella sp. CD2 |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.8 | Cu2+ | - |
Shigella sp. CD2 | |
3.1.3.8 | EDTA | - |
Shigella sp. CD2 | |
3.1.3.8 | Fe2+ | - |
Shigella sp. CD2 | |
3.1.3.8 | additional information | the recombinant enzyme shows resistance to trypsin | Shigella sp. CD2 | |
3.1.3.8 | Zn2+ | - |
Shigella sp. CD2 | |
3.1.3.26 | Cu2+ | - |
Shigella sp. CD2 | |
3.1.3.26 | EDTA | - |
Shigella sp. CD2 | |
3.1.3.26 | Fe2+ | - |
Shigella sp. CD2 | |
3.1.3.26 | additional information | the recombinant enzyme shows resistance to trypsin | Shigella sp. CD2 | |
3.1.3.26 | Zn2+ | - |
Shigella sp. CD2 | |
3.1.3.72 | Cu2+ | - |
Shigella sp. CD2 | |
3.1.3.72 | EDTA | - |
Shigella sp. CD2 | |
3.1.3.72 | Fe2+ | - |
Shigella sp. CD2 | |
3.1.3.72 | additional information | the recombinant enzyme shows resistance to trypsin | Shigella sp. CD2 | |
3.1.3.72 | Zn2+ | - |
Shigella sp. CD2 |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.8 | 0.18 | - |
myo-inositol hexakisphosphate | pH 5.5, 37°C, recombinant nonglycosylated enzyme expressed from Escherichia coli | Shigella sp. CD2 | |
3.1.3.8 | 0.22 | - |
myo-inositol hexakisphosphate | pH 5.5, 37°C, recombinant glycosylated enzyme expressed from Pichia pastoris | Shigella sp. CD2 | |
3.1.3.26 | 0.18 | - |
myo-inositol hexakisphosphate | pH 5.5, 37°C, recombinant nonglycosylated enzyme expressed from Escherichia coli | Shigella sp. CD2 | |
3.1.3.26 | 0.22 | - |
myo-inositol hexakisphosphate | pH 5.5, 37°C, recombinant glycosylated enzyme expressed from Pichia pastoris | Shigella sp. CD2 | |
3.1.3.72 | 0.18 | - |
myo-inositol hexakisphosphate | pH 5.5, 37°C, recombinant non-glycosylated enzyme expressed from Escherichia coli | Shigella sp. CD2 | |
3.1.3.72 | 0.22 | - |
myo-inositol hexakisphosphate | pH 5.5, 37°C, recombinant glycosylated enzyme expressed from Pichia pastoris | Shigella sp. CD2 |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.3.8 | extracellular | - |
Shigella sp. CD2 | - |
- |
3.1.3.26 | extracellular | - |
Shigella sp. CD2 | - |
- |
3.1.3.72 | extracellular | - |
Shigella sp. CD2 | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.8 | Ca2+ | activates | Shigella sp. CD2 | |
3.1.3.8 | Mg2+ | activates | Shigella sp. CD2 | |
3.1.3.8 | Mn2+ | activates | Shigella sp. CD2 | |
3.1.3.26 | Ca2+ | activates | Shigella sp. CD2 | |
3.1.3.26 | Mg2+ | activates | Shigella sp. CD2 | |
3.1.3.26 | Mn2+ | activates | Shigella sp. CD2 | |
3.1.3.72 | Ca2+ | activates | Shigella sp. CD2 | |
3.1.3.72 | Mg2+ | activates | Shigella sp. CD2 | |
3.1.3.72 | Mn2+ | activates | Shigella sp. CD2 |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.8 | myo-inositol hexakisphosphate + H2O | Shigella sp. CD2 | phosphate cleavage position is not determined, cf. EC 3.1.3.8, 3.1.3.26, and 3.1.3.72 | 1D-myo-inositol pentakisphosphate + phosphate | - |
? | |
3.1.3.26 | myo-inositol hexakisphosphate + H2O | Shigella sp. CD2 | phosphate cleavage position is not determined, cf. EC 3.1.3.8, 3.1.3.26, and 3.1.3.72 | 1D-myo-inositol pentakisphosphate + phosphate | - |
? | |
3.1.3.72 | myo-inositol hexakisphosphate + H2O | Shigella sp. CD2 | phosphate cleavage position is not determined, cf. EC 3.1.3.8, 3.1.3.26, and 3.1.3.72 | 1D-myo-inositol pentakisphosphate + phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.3.8 | Shigella sp. CD2 | F6IQ86 | isolated from wheat rhizosphere | - |
3.1.3.26 | Shigella sp. CD2 | F6IQ86 | isolated from wheat rhizosphere | - |
3.1.3.72 | Shigella sp. CD2 | F6IQ86 | isolated from wheat rhizosphere | - |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.1.3.8 | glycoprotein | enzyme AppAS contains three potential sites of N-glycosylation, recombinant nontagged enzyme from Pichia pastoris strain GS115(his4) is glycosylated, deglycosylation of rAppAP is carried out using Endo H deglycosylase | Shigella sp. CD2 |
3.1.3.26 | glycoprotein | enzyme AppAS contains three potential sites of N-glycosylation, recombinant nontagged enzyme from Pichia pastoris strain GS115(his4) is glycosylated, deglycosylation of rAppAP is carried out using Endo H deglycosylase | Shigella sp. CD2 |
3.1.3.72 | glycoprotein | enzyme AppAS contains three potential sites of N-glycosylation, recombinant nontagged enzyme from Pichia pastoris strain GS115(his4) is glycosylated, deglycosylation of rAppAP is carried out using Endo H deglycosylase | Shigella sp. CD2 |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.3.8 | recombinant nonglycosylated His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, recombinant glycosylated nontagged enzyme from Pichia pastoris strain GS115(his4) by cation exchange chromatography | Shigella sp. CD2 |
3.1.3.26 | recombinant nonglycosylated His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, recombinant glycosylated nontagged enzyme from Pichia pastoris strain GS115(his4) by cation exchange chromatography | Shigella sp. CD2 |
3.1.3.72 | recombinant non-glycosylated His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, recombinant glycosylated nontagged enzyme from Pichia pastoris strain GS115(his4) by cation exchange chromatography | Shigella sp. CD2 |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.3.8 | 967 | - |
purified recombinant glycosylated enzyme expressed from Pichia pastoris, pH 5.5, 37°C | Shigella sp. CD2 |
3.1.3.8 | 2982 | - |
purified recombinant nonglycosylated enzyme expressed from Escherichia coli, pH 5.5, 37°C | Shigella sp. CD2 |
3.1.3.26 | 967 | - |
purified recombinant glycosylated enzyme expressed from Pichia pastoris, pH 5.5, 37°C | Shigella sp. CD2 |
3.1.3.26 | 2982 | - |
purified recombinant nonglycosylated enzyme expressed from Escherichia coli, pH 5.5, 37°C | Shigella sp. CD2 |
3.1.3.72 | 967 | - |
purified recombinant glycosylated enzyme expressed from Pichia pastoris, pH 5.5, 37°C | Shigella sp. CD2 |
3.1.3.72 | 2982 | - |
purified recombinant non-glycosylated enzyme expressed from Escherichia coli, pH 5.5, 37°C | Shigella sp. CD2 |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.8 | additional information | the enzyme shows no or poor activity with ATP, ADP, 4-nitrophenyl phosphate, diphosphate, glucose 6-phosphate, and fructose 6-phosphate | Shigella sp. CD2 | ? | - |
? | |
3.1.3.8 | myo-inositol hexakisphosphate + H2O | phosphate cleavage position is not determined, cf. EC 3.1.3.8, 3.1.3.26, and 3.1.3.72 | Shigella sp. CD2 | 1D-myo-inositol pentakisphosphate + phosphate | - |
? | |
3.1.3.26 | additional information | the enzyme shows no or poor activity with ATP, ADP, 4-nitrophenyl phosphate, diphosphate, glucose 6-phosphate, and fructose 6-phosphate | Shigella sp. CD2 | ? | - |
? | |
3.1.3.26 | myo-inositol hexakisphosphate + H2O | phosphate cleavage position is not determined, cf. EC 3.1.3.8, 3.1.3.26, and 3.1.3.72 | Shigella sp. CD2 | 1D-myo-inositol pentakisphosphate + phosphate | - |
? | |
3.1.3.72 | additional information | the enzyme shows no or poor activity with ATP, ADP, 4-nitrophenyl phosphate, diphosphate, glucose 6-phosphate, and fructose 6-phosphate | Shigella sp. CD2 | ? | - |
? | |
3.1.3.72 | myo-inositol hexakisphosphate + H2O | phosphate cleavage position is not determined, cf. EC 3.1.3.8, 3.1.3.26, and 3.1.3.72 | Shigella sp. CD2 | 1D-myo-inositol pentakisphosphate + phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.3.8 | ? | x * 59000-65000, recombinant glycosylated enzyme from Pichia pastoris, SDS-PAGE, x * 45000, recombinant deglycosylated enzyme from Pichia pastoris, SDS-PAGE | Shigella sp. CD2 |
3.1.3.8 | More | AppAS contains three potential sites of N-glycosylation, a putative signal peptide of 22 amino acids at N-terminal end and 8 cysteine residues among which 99-130, 200-210, 404-413 are the most possible disulfide bond pairs. The calculated molecular mass of the protein with and without the signal sequence are about 47 and 45 kDa, respectively | Shigella sp. CD2 |
3.1.3.26 | ? | x * 59000-65000, recombinant glycosylated enzyme from Pichia pastoris, SDS-PAGE, x * 45000, recombinant deglycosylated enzyme from Pichia pastoris, SDS-PAGE | Shigella sp. CD2 |
3.1.3.26 | More | AppAS contains three potential sites of N-glycosylation, a putative signal peptide of 22 amino acids at N-terminal end and 8 cysteine residues among which 99-130, 200-210, 404-413 are the most possible disulfide bond pairs. The calculated molecular mass of the protein with and without the signal sequence are about 47 and 45 kDa, respectively | Shigella sp. CD2 |
3.1.3.72 | ? | x * 59000-65000, recombinant glycosylated enzyme from Pichia pastoris, SDS-PAGE, x * 45000, recombinant deglycosylated enzyme from Pichia pastoris, SDS-PAGE | Shigella sp. CD2 |
3.1.3.72 | More | AppAS contains three potential sites of N-glycosylation, a putative signal peptide of 22 amino acids at N-terminal end and 8 cysteine residues among which 99-130, 200-210, 404-413 are the most possible disulfide bond pairs. The calculated molecular mass of the protein with and without the signal sequence are about 47 and 45 kDa, respectively | Shigella sp. CD2 |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.3.8 | AppA | - |
Shigella sp. CD2 |
3.1.3.8 | AppAS | - |
Shigella sp. CD2 |
3.1.3.8 | More | cf. EC 3.1.3.26 and 3.1.3.72 | Shigella sp. CD2 |
3.1.3.8 | phyS | - |
Shigella sp. CD2 |
3.1.3.8 | phytase | - |
Shigella sp. CD2 |
3.1.3.26 | AppA | - |
Shigella sp. CD2 |
3.1.3.26 | AppAS | - |
Shigella sp. CD2 |
3.1.3.26 | More | cf. EC 3.1.3.8 and 3.1.3.72 | Shigella sp. CD2 |
3.1.3.26 | phyS | - |
Shigella sp. CD2 |
3.1.3.26 | phytase | - |
Shigella sp. CD2 |
3.1.3.72 | AppA | - |
Shigella sp. CD2 |
3.1.3.72 | AppAS | - |
Shigella sp. CD2 |
3.1.3.72 | More | cf. EC 3.1.3.8 and 3.1.3.26 | Shigella sp. CD2 |
3.1.3.72 | phyS | - |
Shigella sp. CD2 |
3.1.3.72 | phytase | - |
Shigella sp. CD2 |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.8 | 60 | - |
recombinant enzyme | Shigella sp. CD2 |
3.1.3.26 | 60 | - |
recombinant enzyme | Shigella sp. CD2 |
3.1.3.72 | 60 | - |
recombinant enzyme | Shigella sp. CD2 |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.8 | 10 | 80 | and below, activity profile overview, recombinant enzymes | Shigella sp. CD2 |
3.1.3.26 | 10 | 80 | and below, activity profile overview, recombinant enzymes | Shigella sp. CD2 |
3.1.3.72 | 10 | 80 | and below, activity profile overview, recombinant enzymes | Shigella sp. CD2 |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.8 | 720 | - |
myo-inositol hexakisphosphate | pH 5.5, 37°C, recombinant glycosylated enzyme expressed from Pichia pastoris | Shigella sp. CD2 | |
3.1.3.8 | 2230 | - |
myo-inositol hexakisphosphate | pH 5.5, 37°C, recombinant nonglycosylated enzyme expressed from Escherichia coli | Shigella sp. CD2 | |
3.1.3.26 | 720 | - |
myo-inositol hexakisphosphate | pH 5.5, 37°C, recombinant glycosylated enzyme expressed from Pichia pastoris | Shigella sp. CD2 | |
3.1.3.26 | 2230 | - |
myo-inositol hexakisphosphate | pH 5.5, 37°C, recombinant nonglycosylated enzyme expressed from Escherichia coli | Shigella sp. CD2 | |
3.1.3.72 | 720 | - |
myo-inositol hexakisphosphate | pH 5.5, 37°C, recombinant glycosylated enzyme expressed from Pichia pastoris | Shigella sp. CD2 | |
3.1.3.72 | 2230 | - |
myo-inositol hexakisphosphate | pH 5.5, 37°C, recombinant non-glycosylated enzyme expressed from Escherichia coli | Shigella sp. CD2 |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.3.8 | 5.5 | - |
recombinant enzyme | Shigella sp. CD2 |
3.1.3.26 | 5.5 | - |
recombinant enzyme | Shigella sp. CD2 |
3.1.3.72 | 5.5 | - |
recombinant enzyme | Shigella sp. CD2 |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.1.3.8 | 2.5 | 8.5 | activity range, profile overview, over 50% of activity at pH 3.5-6.5 | Shigella sp. CD2 |
3.1.3.26 | 2.5 | 8.5 | activity range, profile overview, over 50% of activity at pH 3.5-6.5 | Shigella sp. CD2 |
3.1.3.72 | 2.5 | 8.5 | activity range, profile overview, over 50% of activity at pH 3.5-6.5 | Shigella sp. CD2 |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.8 | 3272.7 | - |
myo-inositol hexakisphosphate | pH 5.5, 37°C, recombinant glycosylated enzyme expressed from Pichia pastoris | Shigella sp. CD2 | |
3.1.3.8 | 12389 | - |
myo-inositol hexakisphosphate | pH 5.5, 37°C, recombinant nonglycosylated enzyme expressed from Escherichia coli | Shigella sp. CD2 | |
3.1.3.26 | 3272.7 | - |
myo-inositol hexakisphosphate | pH 5.5, 37°C, recombinant glycosylated enzyme expressed from Pichia pastoris | Shigella sp. CD2 | |
3.1.3.26 | 12389 | - |
myo-inositol hexakisphosphate | pH 5.5, 37°C, recombinant nonglycosylated enzyme expressed from Escherichia coli | Shigella sp. CD2 | |
3.1.3.72 | 3272.7 | - |
myo-inositol hexakisphosphate | pH 5.5, 37°C, recombinant glycosylated enzyme expressed from Pichia pastoris | Shigella sp. CD2 | |
3.1.3.72 | 12389 | - |
myo-inositol hexakisphosphate | pH 5.5, 37°C, recombinant non-glycosylated enzyme expressed from Escherichia coli | Shigella sp. CD2 |