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Literature summary extracted from

  • Jeong, H.Y.; Nguyen, H.P.; Eom, S.H.; Lee, C.
    Integrative analysis of pectin methylesterase (PME) and PME inhibitors in tomato (Solanum lycopersicum) identification, tissue-specific expression, and biochemical characterization (2018), Plant Physiol. Biochem., 132, 557-565 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.11 in the tomato genome, there exists 79 PMEs with temporally and spatially regulated expression, quantitative realtime PCR expression analysis Solanum lycopersicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.11 pectin methylesterase inhibitor PMEI, two tomato PMEIs, SolycPMEI13 and SolycPMEI14, exhibit PMEI activities and inhibit enzyme PME. In the tomato genome, there exist 48 PMEI genes with temporally and spatially regulated expression, realtime PCR expression analysis show tissue-specific expression. Recombinant expression of MBP-tagged PMEIs in Escherichia coli strain BL21 CodonPlus (DE3)-RIPL. The highest PME activity is detected in samples isolated from green fruits, whereas soluble proteins isolated from green and red fruit possess the lowest PMEI activity Solanum lycopersicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.11 pectin + n H2O Solanum lycopersicum
-
 n methanol + pectate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.11 Solanum lycopersicum
-
 cv. Moneymaker, in the tomato genome, there exists 79 PMEs with temporally and spatially regulated expression
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.11 fruit
-
 Solanum lycopersicum
-
3.1.1.11 leaf
-
 Solanum lycopersicum
-
3.1.1.11 additional information the highest PME activity is detected in samples isolated from green fruits, whereas soluble proteins isolated from green and red fruit possess the lowest PMEI inhibitor activity. Root and stems possess high PME activities, while low activities are detected in leaf tissues, suggesting that vegetative tissues also undergo dynamic pectin modification Solanum lycopersicum
-
3.1.1.11 root
-
 Solanum lycopersicum
-
3.1.1.11 stem
-
 Solanum lycopersicum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.11 pectin + n H2O
-
 Solanum lycopersicum n methanol + pectate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.11 PME
-
 Solanum lycopersicum

General Information

EC Number General Information Comment Organism
3.1.1.11 evolution the enzyme belongs to the family of class 8 carbohydrate esterases. PMEs are classified into either Type-1 (with a PMEI domain at the N-terminus) or Type-2 (no PMEI domain). The highest PME activity is detected in samples isolated from green fruits, whereas soluble proteins isolated from green and red fruit possess the lowest PMEI inhibitor activity. Root and stems possess high PME activities, while low activities are detected in leaf tissues, suggesting that vegetative tissues also undergo dynamic pectin modification Solanum lycopersicum