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Literature summary extracted from

  • Selvan, N.; Williamson, R.; Mariappa, D.; Campbell, D.G.; Gourlay, R.; Ferenbach, A.T.; Aristotelous, T.; Hopkins-Navratilova, I.; Trost, M.; van Aalten, D.M.F.
    A mutant O-GlcNAcase enriches Drosophila developmental regulators (2017), Nat. Chem. Biol., 13, 882-887 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.169 expressed in Escherichia coli BL21-Gold (DE3) pLyssS cells Drosophila melanogaster

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.169 D298N inactive mutant which retains the ability to bind to O-GlcNAcylated peptides Drosophila melanogaster
3.2.1.169 D401A the mutant displays 5fold increase in KM and 2400fold decrease in kcat compared to the wild type enzyme Drosophila melanogaster

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.169 [protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H2O Drosophila melanogaster
-
 [protein]-L-serine + N-acetyl-D-glucosamine
-
 ?
3.2.1.169 [protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonine + H2O Drosophila melanogaster
-
 [protein]-L-threonine + N-acetyl-D-glucosamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.169 Drosophila melanogaster
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.169 His Trap column chromatography Drosophila melanogaster

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.169 embryo
-
 Drosophila melanogaster
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.169 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide + H2O
-
 Drosophila melanogaster 4-methylumbelliferone + N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.169 VPST(GlcNAc)MSAN + H2O
-
 Drosophila melanogaster VPSTMSAN + N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.169 [host cell factor protein]-3-O-(N-acetyl-beta-D-hexosaminyl)-L-threonine + H2O the sequence of host cell factor protein is IVPSVtASHSLR, t indicates the HexNAc-modified residue Drosophila melanogaster [host cell factor protein]-L-threonine + N-acetyl-D-hexosamine
-
 ?
3.2.1.169 [nucleoporin 153]-3-O-(N-acetyl-beta-D-hexosaminyl)-L-serine + H2O the seque4nce of nucleoporin 153 is FDLIIKPTVPVVsKPSTTDPIQSSK. s indicates the HexNAc-modified residue Drosophila melanogaster [nucleoporin 153]-L-serine + N-acetyl-D-hexosamine
-
 ?
3.2.1.169 [protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H2O
-
 Drosophila melanogaster [protein]-L-serine + N-acetyl-D-glucosamine
-
 ?
3.2.1.169 [protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonine + H2O
-
 Drosophila melanogaster [protein]-L-threonine + N-acetyl-D-glucosamine
-
 ?
3.2.1.169 [TAB1 protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H2O
-
 Drosophila melanogaster [TAB1 protein]-L-serine + N-acetyl-D-glucosamine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.169 O-GlcNAc hydrolase
-
 Drosophila melanogaster
3.2.1.169 O-GlcNAcase
-
 Drosophila melanogaster
3.2.1.169 OGA
-
 Drosophila melanogaster