EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.363 | expressed in Escherichia coli BL21(DE3) cells | Panax ginseng |
2.4.1.366 | expressed in Escherichia coli BL21(DE3) cells | Panax ginseng |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.4.1.363 | A40S | the mutant of isoform UGTPg1 shows about wild type activity towards (20S)-protopanaxatriol | Panax ginseng |
2.4.1.363 | F85L | the mutant of isoform UGTPg1 shows about wild type activity towards (20S)-protopanaxatriol | Panax ginseng |
2.4.1.363 | H144Y | the mutant of isoform UGTPg1 is inactive towards (20S)-protopanaxatriol | Panax ginseng |
2.4.1.363 | L38F | the mutant of isoform UGTPg1 shows about wild type activity towards (20S)-protopanaxatriol | Panax ginseng |
2.4.1.363 | Q388H | the mutant of isoform UGTPg1 shows about wild type activity towards (20S)-protopanaxatriol | Panax ginseng |
2.4.1.363 | T134I | the mutant of isoform UGTPg1 shows about wild type activity towards (20S)-protopanaxatriol | Panax ginseng |
2.4.1.363 | T142A | the mutant of isoform UGTPg103 is inactive towards (20S)-protopanaxatriol | Panax ginseng |
2.4.1.363 | T142A/S186L | the mutant of isoform UGTPg103 is inactive towards (20S)-protopanaxatriol | Panax ginseng |
2.4.1.363 | T142A/S186L/R338G | the mutant of isoform UGTPg103 has activity to glycosylate the C6-OH of (20S)-protopanaxatriol | Panax ginseng |
2.4.1.367 | A142T | the mutant of isoform UGTPg100 is inactive towards (20S)-protopanaxatriol | Panax ginseng |
2.4.1.367 | G338R | the mutant of isoform UGTPg100 is inactive towards (20S)-protopanaxatriol | Panax ginseng |
2.4.1.367 | L186S | the mutant of isoform UGTPg100 is inactive towards (20S)-protopanaxatriol | Panax ginseng |
2.4.1.367 | W205R | the mutant of isoform UGTPg100 remains active with (20S)-protopanaxatriol | Panax ginseng |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.363 | 0.104 | - |
(20S)-protopanaxadiol | isoform UGTPg1, at pH 8.0 and 35°C | Panax ginseng | |
2.4.1.367 | 0.153 | - |
(20S)-protopanaxatriol | at pH 8.0 and 35°C | Panax ginseng |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.363 | UDP-alpha-D-glucose + (20S)-protopanaxadiol | Panax ginseng | - |
UDP + ginsenoside C-K | - |
? | |
2.4.1.366 | UDP-alpha-D-glucose + ginsenoside F1 | Panax ginseng | - |
UDP + (20S)-ginsenoside Rg1 | - |
? | |
2.4.1.367 | UDP-alpha-D-glucose + (20S)-protopanaxatriol | Panax ginseng | - |
UDP + ginsenoside Rh1 | - |
? | |
2.4.1.367 | UDP-alpha-D-glucose + ginsenoside F1 | Panax ginseng | - |
UDP + (20S)-ginsenoside Rg1 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.363 | Panax ginseng | - |
- |
- |
2.4.1.366 | Panax ginseng | - |
- |
- |
2.4.1.367 | Panax ginseng | A0A0K0PVW1 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.363 | additional information | isoforms UGTPg102 and UGTPg103 have no detectable activity on (20S)-protopanaxatriol | Panax ginseng | ? | - |
? | |
2.4.1.363 | UDP-alpha-D-glucose + (20S)-protopanaxadiol | - |
Panax ginseng | UDP + ginsenoside C-K | - |
? | |
2.4.1.363 | UDP-alpha-D-glucose + (20S)-protopanaxadiol | the reaction is catalyzed by the enzyme isoform UGTPg1 | Panax ginseng | UDP + ginsenoside C-K | - |
? | |
2.4.1.363 | UDP-alpha-D-glucose + (20S)-protopanaxatriol | the reaction is catalyzed by the enzyme isoform UGTPg1 | Panax ginseng | UDP + ginsenoside F1 | - |
? | |
2.4.1.363 | UDP-alpha-D-glucose + ginsenoside Rg3 | the reaction is catalyzed by the enzyme isoform UGTPg1 | Panax ginseng | UDP + ginsenoside Rd | - |
? | |
2.4.1.363 | UDP-alpha-D-glucose + ginsenoside Rh2 | the reaction is catalyzed by the enzyme isoform UGTPg1 | Panax ginseng | UDP + ginsenoside F2 | - |
? | |
2.4.1.366 | additional information | isoform UGTPg101 cannot glycosylate the C20-OH of ginsenosides Rh1 or Rg2 and the C6-OH of (20S)-protopanaxatriol | Panax ginseng | ? | - |
? | |
2.4.1.366 | UDP-alpha-D-glucose + (20S)-protopanaxadiol | - |
Panax ginseng | UDP + ginsenoside C-K | - |
? | |
2.4.1.366 | UDP-alpha-D-glucose + (20S)-protopanaxatriol | - |
Panax ginseng | UDP + ginsenoside F1 | - |
? | |
2.4.1.366 | UDP-alpha-D-glucose + ginsenoside F1 | - |
Panax ginseng | UDP + (20S)-ginsenoside Rg1 | - |
? | |
2.4.1.366 | UDP-alpha-D-glucose + ginsenoside F1 | - |
Panax ginseng | UDP + ginsenoside Rg1 | - |
? | |
2.4.1.366 | UDP-alpha-D-glucose + ginsenoside Rg3 | - |
Panax ginseng | UDP + ginsenoside Rd | - |
? | |
2.4.1.366 | UDP-alpha-D-glucose + ginsenoside Rh2 | - |
Panax ginseng | UDP + ginsenoside F2 | - |
? | |
2.4.1.367 | UDP-alpha-D-glucose + (20S)-protopanaxatriol | - |
Panax ginseng | UDP + ginsenoside Rh1 | - |
? | |
2.4.1.367 | UDP-alpha-D-glucose + ginsenoside F1 | - |
Panax ginseng | UDP + (20S)-ginsenoside Rg1 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.363 | UGT | - |
Panax ginseng |
2.4.1.363 | UGTPg1 | - |
Panax ginseng |
2.4.1.363 | UGTPg1 | isoform | Panax ginseng |
2.4.1.363 | UGTPg102 | isoform | Panax ginseng |
2.4.1.363 | UGTPg103 | isoform | Panax ginseng |
2.4.1.363 | uridine diphosphate glycosyltransferase | - |
Panax ginseng |
2.4.1.366 | UGT | - |
Panax ginseng |
2.4.1.366 | UGTPg101 | isoform | Panax ginseng |
2.4.1.366 | uridine diphosphate glycosyltransferase | - |
Panax ginseng |
2.4.1.367 | UGTPg100 | - |
Panax ginseng |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.363 | 17.9 | - |
(20S)-protopanaxadiol | isoform UGTPg1, at pH 8.0 and 35°C | Panax ginseng | |
2.4.1.367 | 9.1 | - |
(20S)-protopanaxatriol | at pH 8.0 and 35°C | Panax ginseng |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.363 | 170 | - |
(20S)-protopanaxadiol | isoform UGTPg1, at pH 8.0 and 35°C | Panax ginseng | |
2.4.1.367 | 60 | - |
(20S)-protopanaxatriol | at pH 8.0 and 35°C | Panax ginseng |