Literature summary extracted from

  • Wei, W.; Wang, P.; Wei, Y.; Liu, Q.; Yang, C.; Zhao, G.; Yue, J.; Yan, X.; Zhou, Z.
    Characterization of Panax ginseng UDP-glycosyltransferases catalyzing protopanaxatriol and biosyntheses of bioactive ginsenosides F1 and Rh1 in metabolically engineered yeasts (2015), Mol. Plant, 8, 1412-1424 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.363 expressed in Escherichia coli BL21(DE3) cells Panax ginseng
2.4.1.366 expressed in Escherichia coli BL21(DE3) cells Panax ginseng

Engineering

EC Number Protein Variants Comment Organism
2.4.1.367 G338R the mutant of isoform UGTPg100 is inactive towards (20S)-protopanaxatriol Panax ginseng
2.4.1.363 A40S the mutant of isoform UGTPg1 shows about wild type activity towards (20S)-protopanaxatriol Panax ginseng
2.4.1.363 T142A the mutant of isoform UGTPg103 is inactive towards (20S)-protopanaxatriol Panax ginseng
2.4.1.363 F85L the mutant of isoform UGTPg1 shows about wild type activity towards (20S)-protopanaxatriol Panax ginseng
2.4.1.363 T142A/S186L/R338G the mutant of isoform UGTPg103 has activity to glycosylate the C6-OH of (20S)-protopanaxatriol Panax ginseng
2.4.1.363 T142A/S186L the mutant of isoform UGTPg103 is inactive towards (20S)-protopanaxatriol Panax ginseng
2.4.1.363 L38F the mutant of isoform UGTPg1 shows about wild type activity towards (20S)-protopanaxatriol Panax ginseng
2.4.1.363 T134I the mutant of isoform UGTPg1 shows about wild type activity towards (20S)-protopanaxatriol Panax ginseng
2.4.1.363 Q388H the mutant of isoform UGTPg1 shows about wild type activity towards (20S)-protopanaxatriol Panax ginseng
2.4.1.363 H144Y the mutant of isoform UGTPg1 is inactive towards (20S)-protopanaxatriol Panax ginseng
2.4.1.367 A142T the mutant of isoform UGTPg100 is inactive towards (20S)-protopanaxatriol Panax ginseng
2.4.1.367 L186S the mutant of isoform UGTPg100 is inactive towards (20S)-protopanaxatriol Panax ginseng
2.4.1.367 W205R the mutant of isoform UGTPg100 remains active with (20S)-protopanaxatriol Panax ginseng

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.363 0.104
-
(20S)-protopanaxadiol isoform UGTPg1, at pH 8.0 and 35°C Panax ginseng
2.4.1.367 0.153
-
(20S)-protopanaxatriol at pH 8.0 and 35°C Panax ginseng

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.363 UDP-alpha-D-glucose + (20S)-protopanaxadiol Panax ginseng
-
UDP + ginsenoside C-K
-
?
2.4.1.366 UDP-alpha-D-glucose + ginsenoside F1 Panax ginseng
-
UDP + (20S)-ginsenoside Rg1
-
?
2.4.1.367 UDP-alpha-D-glucose + (20S)-protopanaxatriol Panax ginseng
-
UDP + ginsenoside Rh1
-
?
2.4.1.367 UDP-alpha-D-glucose + ginsenoside F1 Panax ginseng
-
UDP + (20S)-ginsenoside Rg1
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.363 Panax ginseng
-
-
-
2.4.1.366 Panax ginseng
-
-
-
2.4.1.367 Panax ginseng A0A0K0PVW1
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.363 additional information isoforms UGTPg102 and UGTPg103 have no detectable activity on (20S)-protopanaxatriol Panax ginseng ?
-
?
2.4.1.366 additional information isoform UGTPg101 cannot glycosylate the C20-OH of ginsenosides Rh1 or Rg2 and the C6-OH of (20S)-protopanaxatriol Panax ginseng ?
-
?
2.4.1.363 UDP-alpha-D-glucose + (20S)-protopanaxadiol
-
Panax ginseng UDP + ginsenoside C-K
-
?
2.4.1.363 UDP-alpha-D-glucose + (20S)-protopanaxadiol the reaction is catalyzed by the enzyme isoform UGTPg1 Panax ginseng UDP + ginsenoside C-K
-
?
2.4.1.366 UDP-alpha-D-glucose + (20S)-protopanaxadiol
-
Panax ginseng UDP + ginsenoside C-K
-
?
2.4.1.367 UDP-alpha-D-glucose + (20S)-protopanaxatriol
-
Panax ginseng UDP + ginsenoside Rh1
-
?
2.4.1.366 UDP-alpha-D-glucose + ginsenoside F1
-
Panax ginseng UDP + (20S)-ginsenoside Rg1
-
?
2.4.1.367 UDP-alpha-D-glucose + ginsenoside F1
-
Panax ginseng UDP + (20S)-ginsenoside Rg1
-
?
2.4.1.363 UDP-alpha-D-glucose + (20S)-protopanaxatriol the reaction is catalyzed by the enzyme isoform UGTPg1 Panax ginseng UDP + ginsenoside F1
-
?
2.4.1.366 UDP-alpha-D-glucose + (20S)-protopanaxatriol
-
Panax ginseng UDP + ginsenoside F1
-
?
2.4.1.366 UDP-alpha-D-glucose + ginsenoside F1
-
Panax ginseng UDP + ginsenoside Rg1
-
?
2.4.1.363 UDP-alpha-D-glucose + ginsenoside Rg3 the reaction is catalyzed by the enzyme isoform UGTPg1 Panax ginseng UDP + ginsenoside Rd
-
?
2.4.1.366 UDP-alpha-D-glucose + ginsenoside Rg3
-
Panax ginseng UDP + ginsenoside Rd
-
?
2.4.1.363 UDP-alpha-D-glucose + ginsenoside Rh2 the reaction is catalyzed by the enzyme isoform UGTPg1 Panax ginseng UDP + ginsenoside F2
-
?
2.4.1.366 UDP-alpha-D-glucose + ginsenoside Rh2
-
Panax ginseng UDP + ginsenoside F2
-
?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.363 UGT
-
Panax ginseng
2.4.1.366 UGT
-
Panax ginseng
2.4.1.363 uridine diphosphate glycosyltransferase
-
Panax ginseng
2.4.1.366 uridine diphosphate glycosyltransferase
-
Panax ginseng
2.4.1.366 UGTPg101 isoform Panax ginseng
2.4.1.367 UGTPg100
-
Panax ginseng
2.4.1.363 UGTPg1
-
Panax ginseng
2.4.1.363 UGTPg1 isoform Panax ginseng
2.4.1.363 UGTPg102 isoform Panax ginseng
2.4.1.363 UGTPg103 isoform Panax ginseng

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.363 17.9
-
(20S)-protopanaxadiol isoform UGTPg1, at pH 8.0 and 35°C Panax ginseng
2.4.1.367 9.1
-
(20S)-protopanaxatriol at pH 8.0 and 35°C Panax ginseng

KCat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.363 170
-
(20S)-protopanaxadiol isoform UGTPg1, at pH 8.0 and 35°C Panax ginseng
2.4.1.367 60
-
(20S)-protopanaxatriol at pH 8.0 and 35°C Panax ginseng