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Literature summary extracted from

  • Mootapally, C.; Nathani, N.; Patel, A.; Jakhesara, S.; Joshi, C.
    Mining of ruminant microbial phytase (RPHY1) from metagenomic data of Mehsani buffalo breed identification, gene cloning, and characterization (2016), J. Mol. Microbiol. Biotechnol., 26, 252-260 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.8 EDTA strong activation Prevotella ruminicola
3.1.3.26 EDTA strong activation Prevotella ruminicola

Application

EC Number Application Comment Organism
3.1.3.8 agriculture the RPHY1 gene mined from rumen proves its promising candidature as a feed supplement enzyme in animal farming Prevotella ruminicola
3.1.3.26 agriculture the RPHY1 gene mined from rumen proves its promising candidature as a feed supplement enzyme in animal farming Prevotella ruminicola

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.8 DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Prevotella ruminicola
3.1.3.26 DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Prevotella ruminicola

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.8 Ca2+ slight inhibition Prevotella ruminicola
3.1.3.8 K+ strong inhibition Prevotella ruminicola
3.1.3.8 Mg2+ strong inhibition Prevotella ruminicola
3.1.3.8 Mn2+ slight inhibition Prevotella ruminicola
3.1.3.8 Na+ slight inhibition Prevotella ruminicola
3.1.3.8 SDS
-
 Prevotella ruminicola
3.1.3.26 Ca2+ slight inhibition Prevotella ruminicola
3.1.3.26 K+ strong inhibition Prevotella ruminicola
3.1.3.26 Mg2+ strong inhibition Prevotella ruminicola
3.1.3.26 Mn2+ slight inhibition Prevotella ruminicola
3.1.3.26 Na+ slight inhibition Prevotella ruminicola
3.1.3.26 SDS
-
 Prevotella ruminicola

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.8 additional information
-
 additional information Michaelis-Menten kinetics Prevotella ruminicola
3.1.3.8 0.757
-
 myo-inositol hexakisphosphate pH 5.0, 55°C, recombinant His-tagged enzyme Prevotella ruminicola
3.1.3.26 additional information
-
 additional information Michaelis-Menten kinetics Prevotella ruminicola
3.1.3.26 0.757
-
 myo-inositol hexakisphosphate pH 5.0, 55°C Prevotella ruminicola

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.8 myo-inositol hexakisphosphate + H2O Prevotella ruminicola phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 1D-myo-inositol pentakisphosphate + phosphate
-
 ?
3.1.3.26 myo-inositol hexakisphosphate + H2O Prevotella ruminicola phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 1D-myo-inositol pentakisphosphate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.8 Prevotella ruminicola A0A125R6G8 Bacteroides ruminicola, isolated from Mehsani buffalo rumen
-
3.1.3.26 Prevotella ruminicola A0A125R6G8 Bacteroides ruminicola, isolated from Mehsani buffalo rumen
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.8 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Prevotella ruminicola
3.1.3.26 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Prevotella ruminicola

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.1.3.8 purified and acetone-precipitated protein is obtained using denaturing and refolding on a nickel affinity chromatography column Prevotella ruminicola
3.1.3.26 purified and acetone-precipitated protein is obtained using denaturing and refolding on a nickel affinity chromatography column Prevotella ruminicola

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.8 1952
-
 purified recombinant His-tagged enzyme, pH 5.0, 55°C Prevotella ruminicola
3.1.3.26 1952
-
 purified recombinant His-tagged enzyme, pH 5.0, 55°C Prevotella ruminicola

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.8 myo-inositol hexakisphosphate + H2O phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 Prevotella ruminicola 1D-myo-inositol pentakisphosphate + phosphate
-
 ?
3.1.3.26 myo-inositol hexakisphosphate + H2O phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 Prevotella ruminicola 1D-myo-inositol pentakisphosphate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.8 ? x * 63000, recombinant His-tagged enzyme, SDS-PAGE, x * 47750, sequence calculation Prevotella ruminicola
3.1.3.26 ? x * 63000, recombinant His-tagged enzyme, SDS-PAGE, x * 47750, sequence calculation Prevotella ruminicola

Synonyms

EC Number Synonyms Comment Organism
3.1.3.8 More cf. EC 3.1.3.26 Prevotella ruminicola
3.1.3.8 myo-inositol hexakisphosphate hydrolase
-
 Prevotella ruminicola
3.1.3.8 phytase
-
 Prevotella ruminicola
3.1.3.8 RPHY1
-
 Prevotella ruminicola
3.1.3.8 ruminant microbial phytase
-
 Prevotella ruminicola
3.1.3.26 More cf. EC 3.1.3.8 Prevotella ruminicola
3.1.3.26 myo-inositol hexakisphosphate hydrolase
-
 Prevotella ruminicola
3.1.3.26 phytase
-
 Prevotella ruminicola
3.1.3.26 RPHY1
-
 Prevotella ruminicola
3.1.3.26 ruminant microbial phytase
-
 Prevotella ruminicola

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.8 55
-
 recombinant His-tagged enzyme Prevotella ruminicola
3.1.3.26 55
-
 recombinant His-tagged enzyme Prevotella ruminicola

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.3.8 25 70 activity range, profile overview Prevotella ruminicola
3.1.3.26 25 70 activity range, profile overview Prevotella ruminicola

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.8
-
 15 purified recombinant His-tagged enzyme, over 50% activity remaining Prevotella ruminicola
3.1.3.26
-
 15 purified recombinant His-tagged enzyme, over 50% activity remaining Prevotella ruminicola

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.8 5
-
 recombinant His-tagged enzyme Prevotella ruminicola
3.1.3.26 5
-
 recombinant His-tagged enzyme Prevotella ruminicola

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.8 1 10 activity range, profile overview Prevotella ruminicola
3.1.3.26 1 10 activity range, profile overview Prevotella ruminicola

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.3.8 1 7 purified recombinant His-tagged enzyme, over 50% activity remaining Prevotella ruminicola
3.1.3.26 1 7 purified recombinant His-tagged enzyme, over 50% activity remaining Prevotella ruminicola

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.3.8 Prevotella ruminicola sequence calculation
-
 7.15
3.1.3.26 Prevotella ruminicola sequence calculation
-
 7.15

General Information

EC Number General Information Comment Organism
3.1.3.8 additional information the enzyme structure possesses a phosphate-binding pocket with residues R54, H55, R58, I61, R146, W272, H315, E316, and V317 involved in phosphate ligand binding Prevotella ruminicola
3.1.3.26 additional information the enzyme structure possesses a phosphate-binding pocket with residues R54, H55, R58, I61, R146, W272, H315, E316, and V317 involved in phosphate ligand binding Prevotella ruminicola