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Literature summary extracted from

  • Wu, D.; Wang, L.; Li, Y.; Zhao, S.; Peng, N.; Liang, Y.
    Heterologous expression and characterization of a thermostable exo-beta-D-glucosaminidase from Aspergillus oryzae (2016), J. Microbiol. Biotechnol., 26, 347-355 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.165 expressed in Pichia pastoris GS115 cells and Escherichia coli Rosetta (DE3) pLysS cells Aspergillus oryzae

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.165 F769W the mutation significantly enhances the specific activity and hydrolysis velocity compared to the wild type enzyme Aspergillus oryzae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.165 Ag+ 47.9% residual activity at 5 mg/ml, complete inhibition at 10 mg/ml Aspergillus oryzae
3.2.1.165 Ca2+ 76.8% residual activity at 10 mg/ml Aspergillus oryzae
3.2.1.165 Co2+ 4.8% residual activity at 10 mg/ml Aspergillus oryzae
3.2.1.165 Cu2+ 19.3% residual activity at 10 mg/ml Aspergillus oryzae
3.2.1.165 EDTA 65% residual activity at 1 mg/ml, complete inhibition at 5 mg/ml Aspergillus oryzae
3.2.1.165 Fe2+ 16.1% residual activity at 10 mg/ml Aspergillus oryzae
3.2.1.165 K+ 84.6% residual activity at 10 mg/ml Aspergillus oryzae
3.2.1.165 Mg2+ 55.8% residual activity at 10 mg/ml Aspergillus oryzae
3.2.1.165 Mn2+ 2.5% residual activity at 5 mg/ml, complete inhibition at 10 mg/ml Aspergillus oryzae
3.2.1.165 Ni2+ 82.9% residual activity at 10 mg/ml Aspergillus oryzae
3.2.1.165 Zn2+ 55.8% residual activity at 10 mg/ml Aspergillus oryzae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.165 18.4
-
 colloidal chitosan wild type enzyme, at pH 5.5 and 50°C Aspergillus oryzae
3.2.1.165 32.9
-
 colloidal chitosan mutant enzyme F769W, at pH 5.5 and 50°C Aspergillus oryzae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.165 additional information not influenced by Ba2+ Aspergillus oryzae

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.165 Aspergillus oryzae W8E1X9
-
-
3.2.1.165 Aspergillus oryzae FL402 W8E1X9
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.165 glycoprotein
-
 Aspergillus oryzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.165 colloidal chitosan + H2O
-
 Aspergillus oryzae beta-D-glucosamine + ?
-
 ?
3.2.1.165 colloidal chitosan + H2O
-
 Aspergillus oryzae FL402 beta-D-glucosamine + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.165 ? x * 100000, SDS-PAGE Aspergillus oryzae
3.2.1.165 ? x * 99200, calculated from amino acid sequence Aspergillus oryzae

Synonyms

EC Number Synonyms Comment Organism
3.2.1.165 CsxA
-
 Aspergillus oryzae
3.2.1.165 exo-beta-D-glucosaminidase
-
 Aspergillus oryzae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.165 50
-
-
 Aspergillus oryzae

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.165 40 60 the recombinant enzyme expressed in Escherichia coli shows more than 45% activity between 40 and 60°C. The recombinant enzyme expressed in Pichia pastoris shows more than 70% activity between 40 and 60°C Aspergillus oryzae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.165 50
-
 the recombinant enzyme from Pichia pastoris remains about 80% activity after 25 h at 50°C, while the recombinant enzyme from Escherichia coli is completely inactive (0.48% residual activity) after 3 h at 50°C Aspergillus oryzae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.165 23.8
-
 colloidal chitosan wild type enzyme, at pH 5.5 and 50°C Aspergillus oryzae
3.2.1.165 102.6
-
 colloidal chitosan mutant enzyme F769W, at pH 5.5 and 50°C Aspergillus oryzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.165 5.5
-
-
 Aspergillus oryzae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.165 4 8 the recombinant enzyme expressed in Escherichia coli shows about 40% activity at pH 4.0, about 80% activity at pH 5.0, 100% activity at pH 5.5, about 90% activity at pH 6.0, and about 20% activity at pH 8.0. The recombinant enzyme expressed in Pichia pastoris shows about 70% activity at pH 4.0, about 90% activity at pH 5.0, 100% activity at pH 5.5, about 95% activity at pH 6.0, and about 20% activity at pH 8.0 Aspergillus oryzae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.165 1.29
-
 colloidal chitosan wild type enzyme, at pH 5.5 and 50°C Aspergillus oryzae
3.2.1.165 3.11
-
 colloidal chitosan mutant enzyme F769W, at pH 5.5 and 50°C Aspergillus oryzae