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Literature summary extracted from

  • Tan, H.; Miao, R.; Liu, T.; Cao, X.; Wu, X.; Xie, L.; Huang, Z.; Peng, W.; Gan, B.
    Enhancing the thermal resistance of a novel acidobacteria-derived phytase by engineering of disulfide bridges (2016), J. Microbiol. Biotechnol., 26, 1717-1722 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.8 DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) uncultured bacterium
3.1.3.26 DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) uncultured bacterium

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.8 T101C/A229C/S307C/V352C/M364C/F398C enhancing the thermal resistance of the acidobacteria-derived phytase by engineering of disulfide bridges, construction of engineered mutant rPhyA6DB by site-directed mutagenesis, overview uncultured bacterium
3.1.3.26 T101C/A229C/S307C/V352C/M364C/F398C enhancing the thermal resistance of the acidobacteria-derived phytase by engineering of disulfide bridges, construction of engineered mutant rPhyA6DB by site-directed mutagenesis, overview uncultured bacterium

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.8 Al3+
-
 uncultured bacterium
3.1.3.8 Ca2+ slight inhibition uncultured bacterium
3.1.3.8 Cu2+
-
 uncultured bacterium
3.1.3.8 Fe2+ slight inhibition uncultured bacterium
3.1.3.8 Fe3+
-
 uncultured bacterium
3.1.3.8 Hg2+ strong inhibition uncultured bacterium
3.1.3.8 Mn2+
-
 uncultured bacterium
3.1.3.8 Pb2+
-
 uncultured bacterium
3.1.3.8 Zn2+
-
 uncultured bacterium
3.1.3.26 Al3+
-
 uncultured bacterium
3.1.3.26 Ca2+ slight inhibition uncultured bacterium
3.1.3.26 Cu2+
-
 uncultured bacterium
3.1.3.26 Fe2+ slight inhibition uncultured bacterium
3.1.3.26 Fe3+
-
 uncultured bacterium
3.1.3.26 Hg2+ strong inhibition uncultured bacterium
3.1.3.26 Mn2+
-
 uncultured bacterium
3.1.3.26 Pb2+
-
 uncultured bacterium
3.1.3.26 Zn2+
-
 uncultured bacterium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.8 0.382
-
 myo-inositol hexakisphosphate recombinant wild-type enzyme, pH 2.5, 40°C uncultured bacterium
3.1.3.8 0.405
-
 myo-inositol hexakisphosphate recombinant mutant enzyme, pH 2.5, 40°C uncultured bacterium
3.1.3.26 0.382
-
 myo-inositol hexakisphosphate recombinant wild-type enzyme, pH 2.5, 40°C uncultured bacterium
3.1.3.26 0.405
-
 myo-inositol hexakisphosphate recombinant mutant enzyme, pH 2.5, 40°C uncultured bacterium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.8 K+ slight activation uncultured bacterium
3.1.3.8 Mg2+ slight activation uncultured bacterium
3.1.3.8 Na+ slight activation uncultured bacterium
3.1.3.26 K+ slight activation uncultured bacterium
3.1.3.26 Mg2+ slight activation uncultured bacterium
3.1.3.26 Na+ slight activation uncultured bacterium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.8 myo-inositol hexakisphosphate + H2O uncultured bacterium phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 1D-myo-inositol pentakisphosphate + phosphate
-
 ?
3.1.3.26 myo-inositol hexakisphosphate + H2O uncultured bacterium phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 1D-myo-inositol pentakisphosphate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.8 uncultured bacterium
-
 isolated from a soil metagenome
-
3.1.3.26 uncultured bacterium
-
 isolated from a soil metagenome
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.8 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography followed by two steps of gel filtration to 95% purity uncultured bacterium
3.1.3.26 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography followed by two steps of gel filtration to 95% purity uncultured bacterium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.8 1792
-
 purified recombinant enzyme, pH 2.5, 37°C uncultured bacterium
3.1.3.8 1844
-
 purified recombinant enzyme, pH 2.5, 40°C uncultured bacterium
3.1.3.26 1792
-
 purified recombinant enzyme, pH 2.5, 37°C uncultured bacterium
3.1.3.26 1844
-
 purified recombinant enzyme, pH 2.5, 40°C uncultured bacterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.8 myo-inositol hexakisphosphate + H2O phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 uncultured bacterium 1D-myo-inositol pentakisphosphate + phosphate
-
 ?
3.1.3.26 myo-inositol hexakisphosphate + H2O phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 uncultured bacterium 1D-myo-inositol pentakisphosphate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.8 ? x * 45741, mass spectrometry uncultured bacterium
3.1.3.8 More three-dimensional structural model, overview uncultured bacterium
3.1.3.26 ? x * 45741, mass spectrometry uncultured bacterium
3.1.3.26 More three-dimensional structural model, overview uncultured bacterium

Synonyms

EC Number Synonyms Comment Organism
3.1.3.8 HAP phytase
-
 uncultured bacterium
3.1.3.8 More cf. EC 3.1.3.26 uncultured bacterium
3.1.3.8 PhyA
-
 uncultured bacterium
3.1.3.8 phytase
-
 uncultured bacterium
3.1.3.26 HAP phytase
-
 uncultured bacterium
3.1.3.26 More cf. EC 3.1.3.8 uncultured bacterium
3.1.3.26 PhyA
-
 uncultured bacterium
3.1.3.26 phytase
-
 uncultured bacterium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.8 40
-
 recombinant enzyme uncultured bacterium
3.1.3.26 40
-
 recombinant enzyme uncultured bacterium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.8 37
-
 purified recombinant His-tagged wild-type enzyme, pH 2.5, half-life is 2 h, and for mutant rPhyA6DB 5.4 h uncultured bacterium
3.1.3.8 48
-
 melting temperature, recombinant His-tagged enzyme uncultured bacterium
3.1.3.8 60
-
 purified recombinant His-tagged wild-type enzyme, pH 7.0, half-life is 5.2 min, and for mutant rPhyA6DB 14.5 min uncultured bacterium
3.1.3.8 80
-
 purified recombinant His-tagged wild-type enzyme, pH 7.0, half-life is 2.3 min, and for mutant rPhyA6DB 7.0 min uncultured bacterium
3.1.3.26 37
-
 purified recombinant His-tagged wild-type enzyme, pH 2.5, half-life is 2 h, and for mutant rPhyA6DB 5.4 h uncultured bacterium
3.1.3.26 48
-
 melting temperature, recombinant His-tagged enzyme uncultured bacterium
3.1.3.26 60
-
 purified recombinant His-tagged wild-type enzyme, pH 7.0, half-life is 5.2 min, and for mutant rPhyA6DB 14.5 min uncultured bacterium
3.1.3.26 80
-
 purified recombinant His-tagged wild-type enzyme, pH 7.0, half-life is 2.3 min, and for mutant rPhyA6DB 7.0 min uncultured bacterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.8 2.5
-
 recombinant enzyme uncultured bacterium
3.1.3.26 2.5
-
 recombinant enzyme uncultured bacterium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.8 1 5 activity range of wild-type and mutant enzyme, profile overview uncultured bacterium
3.1.3.26 1 5 activity range of wild-type and mutant enzyme, profile overview uncultured bacterium

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.3.8 2.5
-
 purified recombinant His-tagged wild-type enzyme, 37°C, half-life is 2 h (for mutant rPhyA6DB 5.4 h) uncultured bacterium
3.1.3.8 7
-
 purified recombinant His-tagged wild-type enzyme, at 60°C the half-life is 5.2 min (for mutant rPhyA6DB 14.5 min), at 80°C the half-life is 2.3 min (for mutant rPhyA6DB 7.0 min) uncultured bacterium
3.1.3.26 2.5
-
 purified recombinant His-tagged wild-type enzyme, 37°C, half-life is 2 h (for mutant rPhyA6DB 5.4 h) uncultured bacterium
3.1.3.26 7
-
 purified recombinant His-tagged wild-type enzyme, at 60°C the half-life is 5.2 min (for mutant rPhyA6DB 14.5 min), at 80°C the half-life is 2.3 min (for mutant rPhyA6DB 7.0 min) uncultured bacterium

General Information

EC Number General Information Comment Organism
3.1.3.8 additional information threedimensional structural model, overview uncultured bacterium
3.1.3.26 additional information three-dimensional structural model, overview uncultured bacterium