EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.3.8 | gene appA_Gw, DNA and amino acid sequence determination and analysis, cloning from metagenome, recombinant overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | uncultured bacterium |
3.1.3.26 | gene appA_Gw, DNA and amino acid sequence determination and analysis, cloning from metagenome, recombinant overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | uncultured bacterium |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.8 | Al3+ | 93% inhibition at 1 mM, complete inhibition at 10 mM | uncultured bacterium | |
3.1.3.8 | Ca2+ | activates at 1-5 mM by up to 20%, inhibits at 10-50 mM | uncultured bacterium | |
3.1.3.8 | Co2+ | 16% inhibition at 5 mM, 49% at 10 mM | uncultured bacterium | |
3.1.3.8 | Cu2+ | 43% inhibition at 5 mM | uncultured bacterium | |
3.1.3.8 | Fe2+ | 64% inhibition at 5 mM | uncultured bacterium | |
3.1.3.8 | Mg2+ | 40% inhibition at 5 mM | uncultured bacterium | |
3.1.3.8 | Mn2+ | activates at 1 mM by 15%, inhibits 16% at 5 mM, 49% at 10 mM, and 85% at 50 mM | uncultured bacterium | |
3.1.3.8 | Ni2+ | 23% inhibition at 1 mM, 39% inhibition at 10 mM | uncultured bacterium | |
3.1.3.8 | Zn2+ | activates at 1 mM by 13%, inhibits 93% at 10 mM | uncultured bacterium | |
3.1.3.26 | Al3+ | 93% inhibition at 1 mM, complete inhibition at 10 mM | uncultured bacterium | |
3.1.3.26 | Ca2+ | activates at 1-5 mM by up to 20%, inhibits at 10-50 mM | uncultured bacterium | |
3.1.3.26 | Co2+ | 16% inhibition at 5 mM, 49% at 10 mM | uncultured bacterium | |
3.1.3.26 | Cu2+ | 43% inhibition at 5 mM | uncultured bacterium | |
3.1.3.26 | Fe2+ | 64% inhibition at 5 mM | uncultured bacterium | |
3.1.3.26 | Mg2+ | 40% inhibition at 5 mM | uncultured bacterium | |
3.1.3.26 | Mn2+ | activates at 1 mM by 15%, inhibits 16% at 5 mM, 49% at 10 mM, and 85% at 50 mM | uncultured bacterium | |
3.1.3.26 | Ni2+ | 23% inhibition at 1 mM, 39% inhibition at 10 mM | uncultured bacterium | |
3.1.3.26 | Zn2+ | activates at 1 mM by 13%, inhibits 93% at 10 mM | uncultured bacterium |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.8 | 0.51 | - |
myo-inositol hexakisphosphate | pH 2.0, 37°C, recombinant enzyme | uncultured bacterium | |
3.1.3.26 | 0.51 | - |
myo-inositol hexakisphosphate | pH 2.0, 37°C, recombinant enzyme | uncultured bacterium |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.3.8 | extracellular | the phytase might be a secreted enzyme located in the extracellular or periplasmic space of the host, residues 1-25 from the N-terminus are predicted to be a putative signal peptide of this phytase | uncultured bacterium | - |
- |
3.1.3.26 | extracellular | the phytase might be a secreted enzyme located in the extracellular or periplasmic space of the host, residues 1-25 from the N-terminus are predicted to be a putative signal peptide of this phytase | uncultured bacterium | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.3.8 | 42000 | - |
about, gel filtration, recombinant His-tagged enzyme | uncultured bacterium |
3.1.3.26 | 42000 | - |
about, gel filtration, recombinant His-tagged enzyme | uncultured bacterium |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.8 | myo-inositol hexakisphosphate + H2O | uncultured bacterium | phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 | 1D-myo-inositol pentakisphosphate + phosphate | - |
? | |
3.1.3.26 | myo-inositol hexakisphosphate + H2O | uncultured bacterium | phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 | 1D-myo-inositol pentakisphosphate + phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.3.8 | uncultured bacterium | - |
metagenome-derived phytase, metagenome derived from subsurface groundwater | - |
3.1.3.26 | uncultured bacterium | - |
metagenome-derived phytase, metagenome derived from subsurface groundwater | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.3.8 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | uncultured bacterium |
3.1.3.26 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | uncultured bacterium |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.3.8 | 298 | - |
purified recombinant enzyme, pH 2.0, 37°C | uncultured bacterium |
3.1.3.26 | 298 | - |
purified recombinant enzyme, pH 2.0, 37°C | uncultured bacterium |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.8 | additional information | lower activity with 4-nitrophenyl phosphate, no or poor activity with AMP, ADP, ATP, GTP, NADP+, glucose 1-phosphate, and glucose 6-phosphate | uncultured bacterium | ? | - |
? | |
3.1.3.8 | myo-inositol hexakisphosphate + H2O | phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 | uncultured bacterium | 1D-myo-inositol pentakisphosphate + phosphate | - |
? | |
3.1.3.26 | additional information | lower activity with 4-nitrophenyl phosphate, no or poor activity with AMP, ADP, ATP, GTP, NADP+, glucose 1-phosphate, and glucose 6-phosphate | uncultured bacterium | ? | - |
? | |
3.1.3.26 | myo-inositol hexakisphosphate + H2O | phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 | uncultured bacterium | 1D-myo-inositol pentakisphosphate + phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.3.8 | monomer | 1 * 42000, recombinant His-tagged enzyme, SDS-PAGE | uncultured bacterium |
3.1.3.26 | monomer | 1 * 42000, recombinant His-tagged enzyme, SDS-PAGE | uncultured bacterium |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.3.8 | appA_Gw | - |
uncultured bacterium |
3.1.3.8 | More | cf. EC 3.1.3.26 | uncultured bacterium |
3.1.3.26 | appA_Gw | - |
uncultured bacterium |
3.1.3.26 | More | cf. EC 3.1.3.8 | uncultured bacterium |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.8 | 45 | - |
- |
uncultured bacterium |
3.1.3.26 | 45 | - |
- |
uncultured bacterium |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.8 | 30 | 70 | activity range, profile overview | uncultured bacterium |
3.1.3.26 | 30 | 70 | activity range, profile overview | uncultured bacterium |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.8 | 60 | - |
purified recombinant enzyme, pH 2.0, after 30 min inactivation, loss of 50% activity after 15 min | uncultured bacterium |
3.1.3.8 | 80 | - |
purified recombinant enzyme, pH 2.0, after 25 min inactivation, loss of 50% activity after 7 min | uncultured bacterium |
3.1.3.8 | 100 | - |
purified recombinant enzyme, pH 2.0, after 20 min inactivation, loss of 50% activity after 4 min | uncultured bacterium |
3.1.3.26 | 60 | - |
purified recombinant enzyme, pH 2.0, after 30 min inactivation, loss of 50% activity after 15 min | uncultured bacterium |
3.1.3.26 | 80 | - |
purified recombinant enzyme, pH 2.0, after 25 min inactivation, loss of 50% activity after 7 min | uncultured bacterium |
3.1.3.26 | 100 | - |
purified recombinant enzyme, pH 2.0, after 20 min inactivation, loss of 50% activity after 4 min | uncultured bacterium |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.3.8 | 2 | - |
- |
uncultured bacterium |
3.1.3.26 | 2 | - |
- |
uncultured bacterium |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.1.3.8 | 1 | 4.5 | activity range, profile overview | uncultured bacterium |
3.1.3.26 | 1 | 4.5 | activity range, profile overview | uncultured bacterium |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.1.3.8 | evolution | the enzyme belongs to the histidine acid phosphatase (HAP) family | uncultured bacterium |
3.1.3.8 | additional information | the phytase seems to possess improved adaptability to the low pH condition caused by the gastric acid in livestock and poultry stomachs | uncultured bacterium |
3.1.3.26 | evolution | the enzyme belongs to the histidine acid phosphatase (HAP) family | uncultured bacterium |
3.1.3.26 | additional information | the phytase seems to possess improved adaptability to the low pH condition caused by the gastric acid in livestock and poultry stomachs | uncultured bacterium |