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Literature summary extracted from

  • Tan, H.; Wu, X.; Xie, L.; Huang, Z.; Gan, B.; Peng, W.
    Cloning, overexpression, and characterization of a metagenome-derived phytase with optimal activity at low pH (2015), J. Microbiol. Biotechnol., 25, 930-935 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.8 gene appA_Gw, DNA and amino acid sequence determination and analysis, cloning from metagenome, recombinant overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) uncultured bacterium
3.1.3.26 gene appA_Gw, DNA and amino acid sequence determination and analysis, cloning from metagenome, recombinant overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) uncultured bacterium

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.8 Al3+ 93% inhibition at 1 mM, complete inhibition at 10 mM uncultured bacterium
3.1.3.8 Ca2+ activates at 1-5 mM by up to 20%, inhibits at 10-50 mM uncultured bacterium
3.1.3.8 Co2+ 16% inhibition at 5 mM, 49% at 10 mM uncultured bacterium
3.1.3.8 Cu2+ 43% inhibition at 5 mM uncultured bacterium
3.1.3.8 Fe2+ 64% inhibition at 5 mM uncultured bacterium
3.1.3.8 Mg2+ 40% inhibition at 5 mM uncultured bacterium
3.1.3.8 Mn2+ activates at 1 mM by 15%, inhibits 16% at 5 mM, 49% at 10 mM, and 85% at 50 mM uncultured bacterium
3.1.3.8 Ni2+ 23% inhibition at 1 mM, 39% inhibition at 10 mM uncultured bacterium
3.1.3.8 Zn2+ activates at 1 mM by 13%, inhibits 93% at 10 mM uncultured bacterium
3.1.3.26 Al3+ 93% inhibition at 1 mM, complete inhibition at 10 mM uncultured bacterium
3.1.3.26 Ca2+ activates at 1-5 mM by up to 20%, inhibits at 10-50 mM uncultured bacterium
3.1.3.26 Co2+ 16% inhibition at 5 mM, 49% at 10 mM uncultured bacterium
3.1.3.26 Cu2+ 43% inhibition at 5 mM uncultured bacterium
3.1.3.26 Fe2+ 64% inhibition at 5 mM uncultured bacterium
3.1.3.26 Mg2+ 40% inhibition at 5 mM uncultured bacterium
3.1.3.26 Mn2+ activates at 1 mM by 15%, inhibits 16% at 5 mM, 49% at 10 mM, and 85% at 50 mM uncultured bacterium
3.1.3.26 Ni2+ 23% inhibition at 1 mM, 39% inhibition at 10 mM uncultured bacterium
3.1.3.26 Zn2+ activates at 1 mM by 13%, inhibits 93% at 10 mM uncultured bacterium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.8 0.51
-
myo-inositol hexakisphosphate pH 2.0, 37°C, recombinant enzyme uncultured bacterium
3.1.3.26 0.51
-
myo-inositol hexakisphosphate pH 2.0, 37°C, recombinant enzyme uncultured bacterium

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.8 extracellular the phytase might be a secreted enzyme located in the extracellular or periplasmic space of the host, residues 1-25 from the N-terminus are predicted to be a putative signal peptide of this phytase uncultured bacterium
-
-
3.1.3.26 extracellular the phytase might be a secreted enzyme located in the extracellular or periplasmic space of the host, residues 1-25 from the N-terminus are predicted to be a putative signal peptide of this phytase uncultured bacterium
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.8 42000
-
about, gel filtration, recombinant His-tagged enzyme uncultured bacterium
3.1.3.26 42000
-
about, gel filtration, recombinant His-tagged enzyme uncultured bacterium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.8 myo-inositol hexakisphosphate + H2O uncultured bacterium phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 1D-myo-inositol pentakisphosphate + phosphate
-
?
3.1.3.26 myo-inositol hexakisphosphate + H2O uncultured bacterium phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 1D-myo-inositol pentakisphosphate + phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.8 uncultured bacterium
-
metagenome-derived phytase, metagenome derived from subsurface groundwater
-
3.1.3.26 uncultured bacterium
-
metagenome-derived phytase, metagenome derived from subsurface groundwater
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.8 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography uncultured bacterium
3.1.3.26 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography uncultured bacterium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.8 298
-
purified recombinant enzyme, pH 2.0, 37°C uncultured bacterium
3.1.3.26 298
-
purified recombinant enzyme, pH 2.0, 37°C uncultured bacterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.8 additional information lower activity with 4-nitrophenyl phosphate, no or poor activity with AMP, ADP, ATP, GTP, NADP+, glucose 1-phosphate, and glucose 6-phosphate uncultured bacterium ?
-
?
3.1.3.8 myo-inositol hexakisphosphate + H2O phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 uncultured bacterium 1D-myo-inositol pentakisphosphate + phosphate
-
?
3.1.3.26 additional information lower activity with 4-nitrophenyl phosphate, no or poor activity with AMP, ADP, ATP, GTP, NADP+, glucose 1-phosphate, and glucose 6-phosphate uncultured bacterium ?
-
?
3.1.3.26 myo-inositol hexakisphosphate + H2O phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 uncultured bacterium 1D-myo-inositol pentakisphosphate + phosphate
-
?

Subunits

EC Number Subunits Comment Organism
3.1.3.8 monomer 1 * 42000, recombinant His-tagged enzyme, SDS-PAGE uncultured bacterium
3.1.3.26 monomer 1 * 42000, recombinant His-tagged enzyme, SDS-PAGE uncultured bacterium

Synonyms

EC Number Synonyms Comment Organism
3.1.3.8 appA_Gw
-
uncultured bacterium
3.1.3.8 More cf. EC 3.1.3.26 uncultured bacterium
3.1.3.26 appA_Gw
-
uncultured bacterium
3.1.3.26 More cf. EC 3.1.3.8 uncultured bacterium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.8 45
-
-
uncultured bacterium
3.1.3.26 45
-
-
uncultured bacterium

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.3.8 30 70 activity range, profile overview uncultured bacterium
3.1.3.26 30 70 activity range, profile overview uncultured bacterium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.8 60
-
purified recombinant enzyme, pH 2.0, after 30 min inactivation, loss of 50% activity after 15 min uncultured bacterium
3.1.3.8 80
-
purified recombinant enzyme, pH 2.0, after 25 min inactivation, loss of 50% activity after 7 min uncultured bacterium
3.1.3.8 100
-
purified recombinant enzyme, pH 2.0, after 20 min inactivation, loss of 50% activity after 4 min uncultured bacterium
3.1.3.26 60
-
purified recombinant enzyme, pH 2.0, after 30 min inactivation, loss of 50% activity after 15 min uncultured bacterium
3.1.3.26 80
-
purified recombinant enzyme, pH 2.0, after 25 min inactivation, loss of 50% activity after 7 min uncultured bacterium
3.1.3.26 100
-
purified recombinant enzyme, pH 2.0, after 20 min inactivation, loss of 50% activity after 4 min uncultured bacterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.8 2
-
-
uncultured bacterium
3.1.3.26 2
-
-
uncultured bacterium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.8 1 4.5 activity range, profile overview uncultured bacterium
3.1.3.26 1 4.5 activity range, profile overview uncultured bacterium

General Information

EC Number General Information Comment Organism
3.1.3.8 evolution the enzyme belongs to the histidine acid phosphatase (HAP) family uncultured bacterium
3.1.3.8 additional information the phytase seems to possess improved adaptability to the low pH condition caused by the gastric acid in livestock and poultry stomachs uncultured bacterium
3.1.3.26 evolution the enzyme belongs to the histidine acid phosphatase (HAP) family uncultured bacterium
3.1.3.26 additional information the phytase seems to possess improved adaptability to the low pH condition caused by the gastric acid in livestock and poultry stomachs uncultured bacterium