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Literature summary extracted from

  • Sato, V.S.; Galdiano Junior, R.F.; Rodrigues, G.R.; Lemos, E.G.; Pizauro Junior, J.M.
    Kinetic characterization of a novel acid ectophosphatase from Enterobacter asburiae (2016), J. Microbiol., 54, 106-113 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.41 arsenate competitive. 10 mM, 39% residual activity Enterobacter asburiae
3.1.3.41 additional information not inhibitory: EDTA, calcium, copper, cobalt, levamisole, and 4-hydroxymercuribenzoate Enterobacter asburiae
3.1.3.41 phosphate competitive. 10 mM, 58% residual activity Enterobacter asburiae
3.1.3.41 vanadate 10 mM, 63% residual activity Enterobacter asburiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.41 0.06
-
4-nitrophenyl phosphate K0.5 value, Hill coefficient 1.2, pH 3.5, 37°C Enterobacter asburiae
3.1.3.41 0.084
-
diphosphate K0.5 value, Hill coefficient 2.3, pH 3.5, 37°C Enterobacter asburiae
3.1.3.41 0.11
-
ATP K0.5 value, Hill coefficient 1.6, pH 3.5, 37°C Enterobacter asburiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.41 membrane
-
Enterobacter asburiae 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.41 Mg2+ 2 mM, 120% of initial activity Enterobacter asburiae

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.41 Enterobacter asburiae
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.41 4-nitrophenyl phosphate + H2O
-
Enterobacter asburiae 4-nitrophenol + phosphate
-
?
3.1.3.41 ATP + H2O
-
Enterobacter asburiae ADP + phosphate
-
?
3.1.3.41 diphosphate + H2O
-
Enterobacter asburiae 2 phosphate
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.41 3.5
-
-
Enterobacter asburiae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.3.41 0.6
-
arsenate pH 3.5, 37°C Enterobacter asburiae
3.1.3.41 1.8
-
phosphate pH 3.5, 37°C Enterobacter asburiae

Expression

EC Number Organism Comment Expression
3.1.3.41 Enterobacter asburiae expression is induced by presence of phosphate up