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Literature summary extracted from
- Functional characterization of a geraniol synthase-encoding gene from Camptotheca acuminata and its application in production of geraniol in Escherichia coli (2016), J. Ind. Microbiol. Biotechnol., 43, 1281-1292 .
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.1.7.11 | synthesis | significant increase in geraniol synthesis is achieved by coexpression of geraniol synthase, mutant S80F of farnesyl diphosphate synthase, and the encoding genes involved in the whole mevalonic acid biosynthetic pathway in Escherichia coli. The additional optimization of medium composition, fermentation time, and addition of metal ions leads to the geraniol production of 48.5 mg/l | Camptotheca acuminata |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.7.11 | expression in Escherichia coli | Camptotheca acuminata |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.7.11 | 0.0895 | - |
geranyl diphosphate | pH 7.5, 32°C | Camptotheca acuminata |  |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.7.11 | Mg2+ | both Mn2+ and Mg2+ are required, optimum concentrations are 1 mM Mg2+ and 0.1 mM Mn2+ | Camptotheca acuminata | |
| 3.1.7.11 | Mn2+ | both Mn2+ and Mg2+ are required, optimum concentrations are 1 mM Mg2+ and 0.1 mM Mn2+ | Camptotheca acuminata | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.7.11 | Camptotheca acuminata | A0A109P1X1 | - |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.7.11 | geranyl diphosphate + H2O | - |
Camptotheca acuminata | geraniol + diphosphate | - |
? | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.7.11 | 5 | 25 | stable for 30 min | Camptotheca acuminata |
| 3.1.7.11 | 45 | - |
30 min, 50% loss of activity | Camptotheca acuminata |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.7.11 | 7.5 | - |
- |
Camptotheca acuminata |
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Release 2023.1 (January 2023)
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